Amino sugars and their related compounds having nucleic acid- scissoring activity.

具有核酸剪切活性的氨基糖及其相关化合物。

• 批准号: 60490024
• 负责人: WATANABE kenji
• 金额: $ 1.6万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60490024/
• 关键词: Aminosugars; D-glucosamine; DNA damage; DNA切断; 活性酸素; マキサム・ギルバート法; pBR322; プラスミド; DNA; DNA切断反応; Dーグルコサミン; 3ーアミノー3ーデオキシアロース

DNA strand scisson, change of ccc-DNA into oc- and linear-DNA and urther fragmented DNA, by various aminosugars was investigated through agarose gel electrophoresis.D-Glucosamine, D-mannosamine, D-galactosamine and D-isoglucosamine had the DNA strand-scissoring activity. Both the C-2 amino group and C-1 aldehyde one of the molecules were indespensable for their activities. D-Glucosamine, auto- claved at 120゜C in agueous solution, had a greater activity than D-glucosamine itself. The reaction was stimulated by Cu^<2+> and inhibited by catalase, SOD and some radical scavengers. Some free radicals, particularly hydroxyl radical, generated during autoxidation of aminosugrs in agueous solution, were thought to be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- lable damage preferentially at pyrimidine residues in the dinucleotide sequence of pyrimidine-purine (5'-> 3').

用琼脂糖凝胶电泳法研究了氨基糖对DNA链的剪切作用，即ccc-DNA向α-DNA和线状DNA的转化，以及进一步的DNA片段化。D-氨基葡萄糖、D-氨基甘露糖、D-氨基半乳糖和D-氨基异葡萄糖具有剪切DNA链的活性。分子中的C-2氨基和C-1醛都具有不可缺少的活性。D-氨基葡萄糖在120 ℃水溶液中高压灭菌后，其活性高于D-氨基葡萄糖本身。Cu^<2+>对该反应有促进作用，而过氧化氢酶、SOD和某些自由基清除剂则抑制该反应.氨基糖在水溶液中自氧化产生的自由基，特别是羟基自由基，被认为参与了DNA链的断裂。D-氨基葡萄糖诱导的碱性-参与DNA链的断裂。D-氨基葡萄糖优先在嘧啶-嘌呤二核苷酸序列中的嘧啶残基（5 '-> 3'）诱导碱性标记损伤。

项目成果

K.Watanabe, N.Kashige, Y.Nakashima, M.Hayashida and K.Sumoto: "DNA strand scission by D-glucosamine and its phosphates in plasmid pBR322" Agricultural and Biological Chemistry. 50. 1459-1465 (1986)

K.Watanabe、N.Kashige、Y.Nakashima、M.Hayashida 和 K.Sumoto：“质粒 pBR322 中 D-葡萄糖胺及其磷酸盐造成的 DNA 链断裂”农业和生物化学。

K.Watanabe;N.Kashige;Y.Nakashima;M.Hayashida;K.Sumoto: Agricultural & Biological Chemistry. 50. 1459-1465 (1986)

K.Watanabe;N.Kashige;Y.Nakashima;M.Hayashida;K.Sumoto：农业

K. Watanabe;T. Sasaki;N. Kashige;K. Ishibashi;Y. Nakashima;M. Hayashiba: Agricultural and Biological Chemistry. 49. 63-70 (1985)

K.渡边；T.

K. Watanabe;N. Kashige;Y. Nakashima;M. Hayashiba;K. Sumoto: Agricultural and Biological Chemistry. 50. 1459-1465 (1986)

K.渡边;N.

K.Watanabe, T.Sasaki, N.Kashige, K.Ishibashi, Y.Nakashima and M.Hayashida: "Mechanism of in vitro inactivation of Lactobacillus phage PL-1 by D-glucosamine" Agricultural and Biological Chemistry. 49. 63-70 (1985)

K.Watanabe、T.Sasaki、N.Kashige、K.Ishibashi、Y.Nakashima 和 M.Hayashida：“D-葡萄糖胺体外灭活乳杆菌噬菌体 PL-1 的机制”农业和生物化学。