Isolation and Action Mechanism of Oxidation Enzyme of Unsaturated Fatty Acids produced in Rice Plants suffered from Stress.

胁迫下水稻产生的不饱和脂肪酸氧化酶的分离及其作用机制。

• 批准号: 61470030
• 负责人: KATO Tadahiro
• 金额: $ 3.33万
• 依托单位: SCIENCE UNIVERSITY OF TOKYO (1987)Tohoku University (1986)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61470030/
• 关键词: Lipoxygenase; Perhydro-unsaturated Fatty Acids; Anti-rice Blast Fungus Activity; ロイコトリエンB_4関連物質; リポキシダーゼ; 細胞毒性

1. RELATIONSHIP BETWEEN INFECTION AND LIPOXYGENASE ACTIVITY IN RICE PLANTSAlthough it has been recognized for a long time that the rice plants become susceptible and resistant depending on the race of the rice blast fungus, its physiological mechanism has remained unsolved until now. After six kinds of rice plants such as Sasanishiki, Homarenishiki and so on were infected with specific race of the fungus to make the corresponding susceptible and resistant lesions, the lipoxygenase activity were assayed. It was revealed that the lipoxygenase activity in all the susceptible lesions increased greatly at 7 days after infection. On the other hands, the activity maxima were observed at 3 days after infection in each case of resistant lesions. These findings demonstrate clearly that formation mechanism of small brown (R-type) and large (S-type) lesions of the infected rice are closely related with the activity of lipoxygenase by which the unsaturated fatty acid is transformed into cytotoxic hydroperoxy derivatives.2. OXIDATION OF SEVERAL UNSATURATED FATTY ACIDS WITH LIPOXYGENASE FROM RICE PLANTSThe following typical unsaturated fatty acids were submitted to the action of lipoxygenase; linolenic acid, <alpha>_- and <gamma>-linoleic acids, arachidonic acid, eicosapentenoic acid, and docosahexenoic acid, respectively. It was found that the liposygenase oxidation proceeded regio and stereospecifically, offording the corresponding perhydro unsaturated fatty acids. These acids are versatile compounds in studying the role of unsaturated fatty acids in the living bodies.3. STRUCTURE AND SYNTHESIS OF THE LTB_4 TYPE COMPOUNDBy the synthetic study, it was demonstrated that the structure of one of the highly oxygenated fatty acid isolated from the infected rice plant was 9,16-dihydroxy- octadeca-10,12,14-trienoic acid.

1.水稻感染与脂氧合酶活性之间的关系虽然人们早已认识到水稻的感病和抗病性取决于稻瘟病菌的小种，但其生理机制至今仍未得到解决。用该菌的特异小种侵染Sasanishiki、Homarenishiki等6种水稻，使其产生相应的感病和抗病病斑后，测定其脂氧合酶活性。结果表明，在感染后7天，所有易感病灶中的脂氧合酶活性均显著升高。另一方面，在每种耐药病变的情况下，在感染后3天观察到活性最大值。这些结果清楚地表明，受感染水稻的小褐（R型）和大褐（S型）病斑的形成机制与脂氧合酶的活性密切相关，脂氧合酶将不饱和脂肪酸转化为具有细胞毒性的过氧氢衍生物.脂肪氧合酶对水稻中几种不饱和脂肪酸的氧化作用以下典型的不饱和脂肪酸分别受到脂肪氧合酶的作用：亚麻酸、<alpha>α-亚油酸和α<gamma>-亚油酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸。结果表明，脂合酶氧化脂肪酸具有区域性和立体专一性，生成相应的全氢不饱和脂肪酸。这些酸是研究不饱和脂肪酸在生物体内作用的通用化合物。3. LTB_4型化合物的结构与合成通过合成研究，证明了从水稻病株中分离的一种高氧脂肪酸的结构为9，16-二羟基十八碳-10，12，14-三烯酸。

项目成果

加藤忠弘: Phytochemistry. 1988. (1988)

加藤忠宏：植物化学，1988。（1988）

加藤忠弘: J. Chem. Soc. Chem. Commun.1986. 743-744 (1986)

加藤忠宏：化学学会。1986 年。

Tetrahedron Letters

• DOI: 10.1038/1831433c0
• 发表时间: 1959
• 期刊: Nature
• 影响因子: 64.8

Tadahiro Kato: "Oxidation of Typical Unsaturated Fatty Acids with Infected Rice Plant Lipoxygenase" Chemistry Lett.1988. (1988)

Tadahiro Kato：“受感染的水稻植物脂氧合酶对典型不饱和脂肪酸的氧化”Chemistry Lett.1988。

加藤忠弘: Chemistry Lett.1988. (1988)

加藤忠宏：化学快报.1988 (1988)。