Developmental Study of New Agricultural Drugs Based on Lipoxygenase Activity

基于脂氧合酶活性的农用新药开发研究

• 批准号: 63840018
• 负责人: KATO Tadahiro
• 金额: $ 2.56万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63840018/
• 关键词: Lipoxygenase; Alpha-Linolenic Acid; Perhydro Fatty Acid; Infected Plants; Pathogens; Anti-pathogen substances; 感染植物; トリヒドロキシ脂肪酸; 酸化型脂肪酸; 抗菌活性; ごま葉枯病; いもち病; 青枯病; リポキシゲナーゼ; カイヨウ病

Concerning the resistancy of rice plants against rice blast fungus, we have already disclosed that oxygenated unsaturated fatty acids are metabolized in the infected rice plant by the action of lipoxygenase, the activity of which increases largely when the rice plants are infected by the pathogen. The oxygenated fatty acids show the strong activity toward the rice blast fungus.In order to find the generality of increments of lipoxygenase activity in the infected higher plants, we examined the activity of lipoxygenase in seven kinds of infected higher plants and observed the strong increase of lipoxygenase activity in several plants, particularly in tomato leaves infected with TMV and other pathogens. -Linolenic acid is oxidized to the corresponding allylic hydroperoxide (I) by the action of lipoxygenase prepared from infected tomato leaves. I was reduced to 9-hydroxy derivative (II) by treatment with NaBH_4.9S absolute configuration of II was elaborated by observation of positive Cotton effect in the CD spectrum of the corresponding benzoate. Several kinds of highly oxygenated fatty acids (III) were also isolated from the -linolenic acid/lipoxygenase oxidation. The hydroperoxide (I) shows the activity to all the pothogens examined while II and III are active to the specific pathogens.

关于水稻植物对稻瘟病菌的抗性，我们已经公开了受感染水稻植物中的含氧不饱和脂肪酸通过脂氧合酶的作用进行代谢，当水稻植物被病原体感染时，脂氧合酶的活性大大增加。含氧脂肪酸对稻瘟病菌表现出较强的活性。为了发现受感染高等植物脂氧合酶活性增加的普遍性，我们检测了七种受感染高等植物的脂氧合酶活性，观察到几种植物中脂氧合酶活性的强烈增加，特别是在感染TMV和其他病原体的番茄叶片中。在从受感染的番茄叶子中制备的脂氧合酶的作用下，亚麻酸被氧化成相应的烯丙型氢过氧化物（I）。通过用NaBH_49S处理将I还原为9-羟基衍生物(II)。通过观察相应苯甲酸酯的CD光谱中的正Cotton效应来阐述II的绝对构型。还从α-亚麻酸/脂氧合酶氧化中分离出几种高度氧化的脂肪酸(III)。氢过氧化物 (I) 对所有检测的病原体均具有活性，而 II 和 III 对特定病原体具有活性。

项目成果

T. Namai, T. Kato, Y. Yamaguchi, and J. Togashi: "Time-course Alteration of Lipoxygenase Activity in Blast-infected Rice Leaves." Ann. Phytopath. Soc. Jpn.56. 26-32 (1990)

T. Namai、T. Kato、Y. Yamaguchi 和 J. Togashi：“受稻瘟病感染的水稻叶片中脂氧合酶活性的时程变化”。

加藤忠弘: "いもち菌に罹病したイネの代謝する高酸化型不飽和脂肪酸の構造(仮題)" “Lipid"を予定している.

加藤忠宏：“受稻瘟病菌影响的大米代谢的高度氧化不饱和脂肪酸的结构（暂定标题）”计划“脂质”。

T. Namai, T. Kato, Y. Yamaguchi, and J. Togashi: "Cytotoxicity and Anti-blast Activity of Hydro-peroxide of Linolenic Acid Produced by in vitro Enzyme Reaction using the Blast-infected Rice Leaves" Ann. Phytopath. Soc. Jpn.(1991)

T. Namai、T. Kato、Y. Yamaguchi 和 J. Togashi：“使用受稻瘟病感染的稻叶进行体外酶反应产生的亚麻酸氢过氧化物的细胞毒性和抗稻瘟病活性”Ann。

加藤忠弘,蛭川俊史,山口仁宏,生井恒雄: 化学と生物. 26. 850-854 (1988)

加藤忠宏、昼川俊文、山口仁、生井恒夫：化学与生物学。26. 850-854 (1988)

Tuneo Namai: "Cytotoxicity and Antiーblast Activity of Hydroperoxide of Linolenic Acid Produced by in vitro Enzyme Reaction Using the Blastーinfected Rice Leaves" Ann.Phytopath.Soc.Jpn.,. 57. (1991)

Tuneo Namai：“使用受稻瘟病感染的稻叶进行体外酶反应产生的亚麻酸氢过氧化物的细胞毒性和抗稻瘟病活性”Ann.Phytopath.Soc.Jpn.，57。（1991）