Development of safer and higher activity herbicides which inhibit acetolactate synthase

（1）醋酸合成酶植物材料的选择：在春季和早期夏天， barley， komatsuna， santo-sai和spinach被选定为良好材料。在夏天，中国小屋和大米种植，在秋天的脊柱和谷仓被推荐。（2）提取和部分纯化方法： In a mixture consisting of 0. 1 M phosphate buffer（pH 7. 0）、1 mM pyruvate、0. 5 mM MgCl_2、0. 5 mM TPP、and 10 M FAD、the Plant material均原化.从20 - 40%饱和的氨基硫酸盐中提取出的同源酸盐溶液溶液溶解了磷酸盐缓冲液，并使用Sephdex G-25栏去盐。（3）酶活性的测定：酶的混合物、20 mM Na-pyruvate、0.5 mM NgCl_2和0.5 mM TPP在30 ° C下孵化，持续30分钟。孵化后，6NH_2SO_4被添加到反应混合物中，在15分钟内保持60 ° C。在生产过程中产生的乙酸酯会去烷氧基化成乙酸的情况下，将生产出添加0.5%的肌酸和5%的萘酚的颜色，并在60 ° C时再次加热。颜色的光学密度在525纳米测量。（4）氯硫醚对植物和动物组织文化的生长也有影响，植物乙酸合成酶的性质，对ALS的抑制模式，并使用36种试剂和3种半合成化学品评估作为ALS抑制剂进行了测试。

(1) Selection of plant materials for acetolactate synthase: In spring and early summer, barley, komatsuna, santo-sai and spinach were selected as good materials. In summer, Chinese cabbage and rice plant and in fall spinach and barley were recommended.(2) Extraction and partial purification methods: In a mixture consisting of 0.1 M phosphate buffer(pH 7.0), 1mM pyruvate, 0.5 mM MgCl_2, 0.5 mM TPP, and 10 M FAD, the plant material is homogenized. The precipitate of the homogenate from 20 - 40 % saturated ammonium sulfate-salting out is solved the phosphate buffer, and de-salted using sephdex G-25 column.(3) Assay of the enzyme activity: The mixture of the enzyme, 20mM Na-pyruvate, 0.5 mM NgCl_2 and 0.5 mM TPP is incubated at 30゜C for 30 min.. After the incubation, 6N H_2SO_4 is added to the reaction mixture,which is kept at 60゜C for 15 min..During the process produced acetolactate is decarboxylated into acetoin, which will produce color with the addition of 0.5 % creatin and 5% naphthol and heating again at 60゜C. The optical density of the color is measured at 525 nm.(4) Also effect of chlorsulfuron on the growth of plant and animal tissue cultures, properties of plant acetolactate synthase, the mode of inhibition of chlorsulfuron on ALS, and using 36 reagents and 3 semi-synthesized chemicals evaluation as ALS inhibitors are examined.