Developmental analysis of the role of call adhesion molecules in animal morphogenesis

细胞粘附分子在动物形态发生中作用的发育分析

• 批准号: 61480021
• 负责人: TAKEICHI Masatoshi
• 金额: $ 3.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480021/
• 关键词: morphogenesis; cell adhesion molecules; cadherins; 細胞選別; アクチン; 中間結合;cDNA

I have isolated cDNAs encoding E-, N- and P-cadherin from cDNA libraries of mRNA derived from mouse and chick embryos. The analysis of the primary structure of these cadherin cDNAs showed that there are homologies in amino acid sequences among the three cadherins, suggesting that these molecules have a common ancester. I then attempted to introduce the cadherin cDNAs into L cells which have little cadherin activity. L cells transfected with the cDNAs synthesized cadherin polypeptides and acquired the Ca^<2+>-dependent aggregating activity. These cells were morphologically transformed from the fibroblast type to the epithelial type.In order to examine the specificity of each cadherin molecule, I mixed L cells expressing E-cadherin with those expressing P-cadherin and allowed them to aggregate. The results showed that these cells aggregate independentely. Therefore, I concluded that each cadherin molecule has a specificity in cell-cell binding.

我已经从小鼠和鸡胚胎mRNA的cDNA文库中分离出编码E-、N-和P-钙粘蛋白的cDNA。一级结构分析表明，三种钙粘蛋白的氨基酸序列具有同源性，提示它们具有共同的祖先。然后，我尝试将钙粘蛋白cDNA导入几乎没有钙粘蛋白活性的L细胞中。转染cDNA的L细胞合成钙粘蛋白多肽，并获得Ca^2+依赖的聚集活性。为了检测各钙粘蛋白分子的特异性，将表达E-钙粘蛋白的L细胞与表达P-钙粘蛋白的L细胞混合，使它们聚集。结果表明，这些细胞是独立聚集的。因此，我得出结论，每个钙粘蛋白分子在细胞-细胞结合中具有特异性。

项目成果

A.Nagafuchi: "Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA" Nature. 329. 341-343 (1987)

A.Nagafuchi：“通过外源引入 E-钙粘蛋白 cDNA 来改变细胞粘附特性”《自然》。

Hatta,K.: Developmental Biology. 120. 215-227 (1987)

Hatta，K.：发育生物学。

Johnson,M.H.: Journal Embryology and Expeimental Morphology. 93. 239-255 (1987)

约翰逊，M.H.：胚胎学和实验形态学杂志。

Duband,J-L: Journal of Cell Biology.

Duband，J-L：细胞生物学杂志。

Hatta,K.: Nature. 320. 447-449 (1986)

Hatta，K.：自然。