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In vitro flower formation of Japanese pear

日本梨的离体成花

基本信息

批准号：
61480041
负责人：
ICHII Takao
金额：
$ 2.24万
依托单位：
Faculty of Agriculture, Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480041/
关键词：
tissue culture flower formaton GA growth retardant Juverility 日長及び温度ニホンナシ花芽分化

项目摘要

In vitro flower formation of Japanese pear was studied using some cultivars with different flowering capacities.Explants formed flowers on their apices, regardless of whether they were prepared from shoot apices of leaf buds, indicating the occurrence of flowering during culture. Flowering occurred predominantly on the explants with a particular range of node numbers, above and below which it diminished. Flowering behavior was closely related to the flowering capacity characteristic of the cultivar from which the explants were prepared. The presence of GA_<4+7> or GA_4 of tne medium tended to inhibit flowering, but GA_3 did not affect if. The growth retardant, S-3307 increased flowering, especially for the explants prepared from the cultivar with low flowering capacity. Day length had little effect on flowering and temperature gave a pronounced effect on flowering. The explants prepared from seedlings did not flower during culture, showing a longer internode length than those from cultivars. Increasing number of subcultures reduced the flowering capcity of cultivars, suggesting an induction of rejuvenaton.

采用不同开花能力的品种对日本梨的离体花形成进行了研究。无论外植体是否由叶芽的茎尖制备，其顶端都形成了花，这表明在培养过程中发生了开花。开花主要发生在具有一定节数范围的外植体上，节数以上和节数以下的外植体开花减少。开花行为与外植体培育品种的开花能力特性密切相关。GA_<4+7>或GA_4的存在有抑制开花的趋势，而GA_3对开花没有影响。生长抑制剂S-3307对开花能力较弱的品种的外植体有明显的促进作用。日照长度对花期影响不大，温度对花期影响显著。从幼苗制备的外植体在培养过程中不开花，节间长度比从栽培品种制备的长。继代培养数量的增加降低了品种的开花能力，表明有返老还老的诱导作用。