Fine structural aspects of the inner ear in relation to the incremental growth of otoliths in fishes

鱼类内耳精细结构与耳石增量生长的关系

• 批准号: 61480065
• 负责人: YAMADA Juro
• 金额: $ 4.16万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480065/
• 关键词: fish otolith; early development; saccular cells; apocrine secretion; microvillous secretion; matrix proteins, calcium binding capacity; Ca結合能; アミノ酸組成; 内耳; 感覚斑; 小嚢上皮; 有機基質

This research project aimed to clarify cellular contributions of the membraneous rabyrinth to the incremental growth of otoliths in fishes from fine structural and biochemical viewpoints. Major subjects investigated and the results obtained are summarized as follows:1. Otolith development in medaka (Oryzias latipes) embryos: The otolith rudiment first appears in the otic vesicle of 2-day embryos as an assemblage of many minute spherules (primordia) measured about 1 um in diameter, close to the presumptive macula. The spherules are observed to originate from apocrine type secretory protrusions of macular cells which are not yet differentiated to sensory cells. The spherules are agglutinated to form the otolith core in 3-day embryos. Accretional growth of the otolith follows around the otolith core by depositing fine filaments secreted through microvilli of the perimacular epithelial cells.2. Ultrastructure of the saccular wall in juvenile tilapia (Orechromis niloticus): Two types of sec … More retion were observed in the cells of the saccular wall; apocrine type secretion of macular cells and liberation of vesicles at the distal end of microvilli of perimacular cells, the former seemed to contribute to the gelatinous layer of the otolithic membrane and the latter to the subcupular meshwork, filaments of which were incorporated into the otolith as the organic constituent. Perimacular cells of the transitional epithelium of the saccular wall, characterized by abundant microvilli, were observed to be actively involved in the otolith growth.3. Biochemical properties of matrix proterns of otoliths in tilapia (Orechromis niloticus): Proteins were extracted with 10 % EDTA from the otoliths. The EDTA- extracted proteins were fractionated into FI-FV by ion-exchange chromatography. PAGE and SDS-PAGE patterns showed that each fraction contained several proteins of 22-185 KDa. Proteins of FIII,IV and V, particularly of FV, were rich in acidic amino acids such as Glu and Asp, and showed the calcium binding capacity. Proteoglycans were found to be contained in FV. Histochemical tests applied to the saccular wall suggested that proteoglycans are actively produced in the perimacular cells at midnight. Less

本研究旨在从精细结构和生物化学角度阐明膜性耳石对鱼类耳石生长的细胞贡献。主要研究内容和结果如下：1.青鳉胚胎的耳石发育：耳石原基首先出现在2天胚胎的耳泡中，作为许多直径约1 μ m的微小小球（原基）的集合体，接近假定的黄斑。观察到小球起源于尚未分化为感觉细胞的黄斑细胞的顶浆分泌型分泌突起。在3日龄胚胎中，小球体凝集形成耳石核心。耳石的生长是围绕着耳石核心，通过黄斑周围上皮细胞微绒毛分泌的细丝沉积而进行的.尼罗罗非鱼幼鱼囊壁的超微结构：两种类型的囊壁 ...更多信息 在囊壁细胞中观察到分泌物，黄斑细胞的顶泌型分泌物和斑周细胞微绒毛远端的小泡释放，前者似乎对耳石膜的凝胶层有贡献，后者似乎对杯下网有贡献，其细丝作为有机成分并入耳石。囊壁移行上皮的斑周细胞具有丰富的微绒毛，参与耳石的生长.罗非鱼耳石基质蛋白的生化特性：用10%EDTA提取耳石中的蛋白质.通过离子交换色谱将EDTA提取的蛋白分级分离成FI-FV。PAGE和SDS-PAGE电泳图谱显示，每个组分都含有22-185 KDa的蛋白质。FIII、IV和V蛋白，特别是FV蛋白，富含Glu和Asp等酸性氨基酸，并显示出钙结合能力。FV中含有蛋白多糖。应用于囊壁的组织化学试验表明，蛋白聚糖在午夜活跃地产生于黄斑周围细胞。少

项目成果

Yamada,Juro: Tokai Univ. Press.Growth and calcification of otoliths and scales in teleosts (in Japanese). In "Phylogenetic Evolution adn Calcification in Marine Organisms" (Eds: Omori, M., Suga, S. and Goto, M.)., (1988)

山田十郎：东海大学

Saitoh, Setsuo: Trans. Amer. Micr. Soc.

齐藤节男：译。

Yamada,Juro: "Electron microscope observations on the early development of otoliths in embryos of medaka (Oryzias latipes )." Trans. Amer. Micr. Soc.

山田十郎：“电子显微镜观察青鳉（Oryzias latipes）胚胎中耳石的早期发育。”

大森・須賀・後藤編山田寿郎他: "海洋生物の石灰化と系統進化" 東海大学出版会, (1988)

大森、菅、后藤、山田敏郎等：《海洋生物的钙化与系统发育进化》东海大学出版社，（1988）

Mugiya,Yasuo: "Phase difference between calcification and organic matrix formation in the diurnal growth of otoliths in the rainbow trout, Salmo gairdneri." Fish. Bull. U. S.85. 395-401 (1987)

Mugiya，Yasuo：“虹鳟鱼耳石昼夜生长中钙化和有机基质形成之间的相位差。”