Gene expression in relation to oogenesis, embryogenesis and development in Bombyx mori

家蚕卵子发生、胚胎发生和发育相关的基因表达

• 批准号: 61480053
• 负责人: KOGA Katsumi
• 金额: $ 4.54万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480053/
• 关键词: Bombyx mori; Egg architecture; Embryogenesis; Chorion proteins; Carotenoid binding protein; Follicular epithelial cells; 卵黄蛋白質; 小形卵突然変異; 卵殻コリオン蛋白質; アルドラーゼアイソザイム; 幼虫型雌蛋白質; 翻訳活性; 組織透過性; 卵形成蛋白質

Gene expression processes were analyzed by using mutants of the domestic silkworm, Bombyx mori, as well as using its supposed ancestor Bombyx mandarina. 1. The eggshell chorion was of interest because of the morphogenesis of its complex structure coded for by the multigene families. Scanning electron microscopy of the mandarina eggshell was very specific at the outermost layer, which seemed to match the outdoor habitation of this species. The mandarina chorion had protein components which were scarcely detected in mori. Southern blot hybridization analysis of the genomic DNA of mandarina differed from those of mori. Thus, mandarina may have a distinct gene set at the chorion locus in comparison with the domestic silkworm. Nevertheless, with respect to the DNA related to the outermost layer proteins, there was a surprising homology between the two species. It may be reasonable to suppose that the outer layer is functionally important because of its direct contact with the environment an … More d the relevant sequences are stable during evolution. Further analysis of the mandarina genome is needed to uncover how the specific outer layer structure is determined. 2. The silkworm larvase were found to have a carotenoid binding protein in the haemolymph. Previously a mutant which lacks the hemolymph carotenoid has been known, interestingly, it was confirmed that the mutant also possesses the protein that was identical to the normal carotenoid binding protein in terms of many biochemical characteristics. The results suggested that there may be, probably in the midgut, a genetically controlled mechanism under which carotenoid molecules are harbored into their specific binding protein. 3. The silkworm has many mutants in which the size and pattern of the egg are modified. Morphological and biochemical analyses of some of such mutants revealed that the follicular epithelial cells have key roles in determining the egg characters. The results provide an approach to understanding the complex process of oogenesis. Less

通过使用家蚕 Bombyx mori 的突变体及其假定的祖先 Bombyx mandarina 来分析基因表达过程。 1. 蛋壳绒毛膜之所以引起人们的兴趣，是因为其复杂结构的形态发生是由多基因家族编码的。扫描电子显微镜观察到的柑橘蛋壳的最外层非常具体，这似乎与该物种的户外栖息地相匹配。柑橘绒毛膜含有在桑蚕中几乎检测不到的蛋白质成分。柑橘基因组 DNA 的 Southern 印迹杂交分析与桑蚕基因组 DNA 不同。因此，与家蚕相比，柑橘的绒毛膜基因座可能具有独特的基因组。然而，就与最外层蛋白质相关的DNA而言，两个物种之间存在令人惊讶的同源性。假设外层在功能上很重要可能是合理的，因为它与环境直接接触，并且相关序列在进化过程中是稳定的。需要对柑橘基因组进行进一步分析，以揭示如何确定特定的外层结构。 2、在家蚕幼虫的血淋巴中发现有类胡萝卜素结合蛋白。此前已知一种缺乏血淋巴类胡萝卜素的突变体，有趣的是，已证实该突变体还具有与正常类胡萝卜素结合蛋白在许多生化特性方面相同的蛋白。结果表明，可能在中肠中存在一种遗传控制机制，在该机制下，类胡萝卜素分子被隐藏在其特异性结合蛋白中。 3. 蚕有许多突变体，其中卵的大小和图案都发生了改变。对其中一些突变体的形态学和生化分析表明，滤泡上皮细胞在决定卵子特性方面发挥着关键作用。这些结果为理解卵子发生的复杂过程提供了一种方法。较少的

项目成果

Yue,Xiong; Bungo,Sakaguchi; Thomas,H.Eickbush: "Gene conversions can generate sequence variations in the late chorion multigene families of Bombyx mori." Genetics.

岳，熊；

河口豊,土井良宏,藤井博: 日蚕雑. 57. 289-297 (1988)

Yutaka Kawaguchi、Yoshihiro Doi、Hiroshi Fujii：日本蚕杂志 57. 289-297 (1988)。

M.,Fitz-Earl;B.,Sakaguchi;M.,Horio;M.,Tsukamoto: J.Med.Entomol.24. 448-457 (1987)

M.，菲茨-厄尔；B.，坂口；M.，堀尾；M.，冢本：J.Med.Entomol.24。

Y.,Mihara;A.,Saito;K.,Koga;B.,Sakaguch: J.Sericult.Sci.Jpn.57. 62-67 (1988)

Y.，Mihara；A.，Saito；K.，Koga；B.，Sakaguch：J.Sericult.Sci.Jpn.57。

Y.Mihara;A.Saito;K.Koga;B.Sakaguchi: J.Sericult.Sci.Jpn.57. 62-67 (1988)

Y.Mihara；A.Saito；K.Koga；B.Sakaguchi：J.Sericult.Sci.Jpn.57。