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Gene expression and its control agents in embryogenesis of Bombyxmori

家蚕胚胎发生中的基因表达及其调控因子

基本信息

批准号：
62304018
负责人：
KOGA Katsumi
金额：
$ 3.84万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The method for extraction of translatable messenger RNA from the eggs of Bombyx mori was established. By using the latter method the mode of switchover of aldolase isozymes, chosen as a marker for gene expression study, could be clarified. Based on the effort to set up the human project, i.e. aiming at the sequencing of the whole genome of man, the scope of preparing the cDNA and genomic libraries of the silkworm was proposed. As to the major yolk protein named egg specific protein (ESP), the relevant transcription, translation and posttranslational processing were precisely analyzed. Moreover, the way of yolkprotein utilization has begun to be uncovered; a specific protease which can degrade ESP was found to be present. This enzyme exhibit a limited break of the ESP chain. This provides the first understanding of the initial steps of yolk protein utilization at the molecular level. The presence of ovarian ecdysteroids has been known. The major species were found to be conjugate types, … More which may be the storage forms and hydrolysis enzymes detected in the present study might have critical roles to provide free, thus active, ecdysteroids during embryogenesis. The initiation of diapause in B. mori is known to be controlled by a single gene pnd^+. The product of the latter gene was suggested to be a protein as separated by two-dimensional gel electrophoresis. The mode of action of the latter gene (and its product) was discussed in terms of protein synthesis and RNA synthesis at the period of diapause onset. As to the break of diapause a signal of the end of the so-called diapause development has been found to be the activation of esterase A. This enzyme was crystallized and revealed to be a time-measuring molecule which activates itself in vitro under the specified conditions minicking the cold activation of diapausing eggs of the silkworm. The nucleotide bases in particular 5-methylcytosine changed the hibernating program of the silkworm eggs. These agents might serve as convenient experimental drugs that disturb the diapasue system to be analyzed. Less

建立了从家蚕卵中提取可翻译信使RNA的方法。通过使用后一种方法的模式，醛缩酶同工酶，选择作为基因表达研究的标记，可以澄清。基于人类工程的建立，即以人类全基因组测序为目标，提出了家蚕cDNA文库和基因组文库制备的范围。对其中的主要卵黄蛋白--卵特异性蛋白（egg specific protein，ESP）的转录、翻译和翻译后加工进行了详细的分析。此外，卵黄蛋白的利用途径也开始被揭示，发现了一种能降解ESP的特异性蛋白酶。这种酶表现出ESP链的有限断裂。这提供了在分子水平上的卵黄蛋白利用的初始步骤的第一个理解。卵巢蜕皮激素的存在是已知的。主要种类为共轭型， ...更多信息这可能是本研究中检测到的储存形式和水解酶可能在胚胎发生过程中提供游离的、因此具有活性的蜕皮类固醇方面发挥关键作用。B滞育的开始。已知家蚕是由单个基因pnd^+控制的。后者基因的产物被认为是一种蛋白质，通过双向凝胶电泳分离。从蛋白质合成和RNA合成两个方面讨论了后一个基因（及其产物）在滞育开始时的作用方式。关于滞育的解除，已发现所谓滞育发育结束的信号是酯酶A的激活。该酶是一种计时分子，在体外特定条件下能自行激活，抑制家蚕滞育卵的冷激活。核苷酸碱基特别是5-甲基胞嘧啶改变了蚕卵的冬眠程序。这些试剂可以作为方便的实验药物，干扰滞育系统进行分析。少