权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on non-specific defence mechanism to bacterial infections in fishes.

鱼类细菌感染非特异性防御机制的研究。

基本信息

批准号：
61480067
负责人：
WAKABAYASHI Hisatsugu
金额：
$ 2.3万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Biological activities of eel complement activated through the alternative pathway were investigated. The normal eel sera were mixed with zymozan, which is known as an activator for the pathway, or formarin-killed cells of selected bacteria and incubated at 25 C for 60 min. The sera were separated from zymosan or the bacterial cells by centrifugation. First, chemotactic activity of the treated sera were determined by a modified Boyden chamber method. The distance of eel neutrophil migration toward the treated sera were significantly different from that toward the heat-inactivated sera. Secondly, leukocytosis-inducing activity was exzamined by intraperitoneally injecting eel with the treated sera. A rapid increase in the number of peripheral neutrophils was observed in the eel and reached a peak 6 - 9 hour after injection. In addition to bactericidal activity demonstrated in our earlier work these chemotactic and leukocytosis-inducing activities of eel complement appeared important in th … More e non-specific defence against bacterial infections.Neutrophils were seperated from the peripheral blood of eel. One ml of undiluted blood were carefully loaded onto a discontinuous density Ficoll-metrizoate ( 1.073, 1.083 and 1.089 in density) solution and the tube was centrifuged for 30 min at 1500 rpm. A fraction containing heavier neutrophils was obtained from the blood of eel injected with bacterial dead cells one day befor blood collection. The 'heavy' neutrophils were not obtained from eel injected with the treated sere, casein or saline (control). They were more active in phagacytosis than the ordinal 'light'ones.Phagocytosis of eel neutrophils isolated from peripheral blood was measured by chemiluminescence (CL) assay. When neutrophils were exposed opsonized bacteria, CL respones increased rapidly. CL response to the non-opsonized bacteria was much lower than that to the opsonized ones. The amount of CL respones became larger in proportion to the concentration of normal serum as an opsonin. CL response in phagocytosis of carp neutrophils was similar to that of eel in pattern, but much lower in intensity. It was found that the CL assay could be applied not only to investigate the magnitude and kinetics of phagaocyte but also to assess the serum opsonic activity. Less

研究了鳗鱼补体经旁路途径激活后的生物学活性。将正常鳗鱼血清与已知为该途径的激活剂的酵母菌或福尔马林杀死的选定细菌的细胞混合，并在25 ℃下孵育60分钟。通过离心将血清与酵母聚糖或细菌细胞分离。首先，通过改良的Boyden室法测定经处理的血清的趋化活性。鳗鱼中性粒细胞向处理血清迁移的距离与向热灭活血清迁移的距离有显著差异。第二，用处理过的血清腹腔注射鳗鱼，检测白细胞生成活性。在注射后6 - 9小时，在鳗鱼中观察到外周中性粒细胞的数量迅速增加，并达到峰值。除了我们早期工作中证实的杀菌活性外，鳗鱼补体的趋化性和白细胞生成诱导活性在免疫抑制中也很重要。 ...更多信息从鳗鲡外周血中分离出中性粒细胞，并进行了体外培养。将1 ml未稀释的血液小心地加载到不连续密度的Ficoll-甲泛影葡胺（密度为1.073、1.083和1.089）溶液中，并将管以1500 rpm离心30分钟。从采血前一天注射细菌死细胞的鳗鱼血液中获得含有较重中性粒细胞的组分。从注射处理过的sere、酪蛋白或盐水（对照）的鳗鱼中未获得“重”中性粒细胞。用化学发光法（CL）测定了鳗鱼外周血中性粒细胞的吞噬功能。当中性粒细胞暴露于调理细菌时，CL反应迅速增加。未调理细菌的CL反应远低于调理细菌。CL反应量作为调理素与正常血清浓度成比例增加。鲤鱼中性粒细胞吞噬的CL反应与鳗鱼相似，但强度低得多。结果表明，CL法不仅可用于研究吞噬细胞的数量和动力学，而且可用于评价血清调理素活性。少