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Kinetics of particle uptake and clearance in fish

鱼类颗粒摄取和清除的动力学

基本信息

批准号：
09460084
负责人：
WAKABAYASHI Hisatsugu
金额：
$ 7.94万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460084/
关键词：
particle uptake clearance skin microinjury macrophage sporozoa fluorescent labeling グルゲア胞子損傷レクチン

项目摘要

1) Fluorescent latex beads were administered to 3-10 g rainbow trout by bath immersion, followed by time series sampling and histological examination. When the live fish exposed to the beads was dipped in 0.05% trypan blue before sampling, the patches of beads were observed at the skin area stained with trypan blue. In the skin examined histologically, there were the beads adhering to the superficial epithelial cells at the edge of wounds. During healing from wounds, the damaged cells and the beads sloughed together from the surface. However, the migrating epithelial cells which would repair the wounds displayed intensive phagocytosis of the beads at the edge of the wounds. The beads were also found in the intracellular gap in the dermis scaled with the migrating epithelium. Thus the microinjury in the skin are implicated as sites of particle uptake during bath immersion.2) Sevcral fluorescent labeling techniques were developed to track infectious organisms on fish hosts. Standardized … More staining methods of Uvitex2B and CFSE were applied for microsporidian and actinospore, respectively. In transmission experiments with labeled spores suggested that micro-sporidian (G.plecoglossi) invaded fish passively through microinjury on the body surface, and that actinospores (T.hovorkai, M.arcticus) invaded actively through gills and/or skin.3) G.plecoglossi spores had lectin-reactive components. Phagocytosis of the spores by ayu head kidney macrophages was significantly inhibited after ConA treatment. Percentage phagocytosis was significantly reduced when the spores were treated with ConA.Hydrogen peroxide production was suppressed by ConA treatment of spores. In the experimental infection of ayu, the number of cysts was smaller in ConA-treated spore administrated group than in untreated control group. It was suggested that ConA could block the binding of G.plecoglossi s spore with ayu macrophages and that their phagocytosis of the spores played an important role in migration of the spore in the infected fish. Less

1)荧光乳胶珠通过浴浸法施用到3-10 g虹鳟鱼，随后进行时间序列取样和组织学检查。当暴露于珠的活鱼在取样前浸入0.05%台盼蓝时，在台盼蓝染色的皮肤区域观察到珠斑。皮肤组织学检查，在伤口边缘的浅表上皮细胞上有小珠粘附。在伤口愈合过程中，受损细胞和珠子一起从表面脱落。然而，将修复伤口的迁移上皮细胞在伤口边缘显示出对珠粒的强烈吞噬作用。珠粒也被发现在真皮细胞内的间隙与迁移上皮缩放。因此，在皮肤中的微损伤牵连的网站，在浴浸泡过程中的颗粒摄取。2）Sevcral荧光标记技术的发展，以跟踪感染性生物体的鱼类宿主。标准化 ...更多信息微孢子虫和放线孢子分别采用Uvitex 2B和CFSE染色。在标记孢子的传播实验中发现，小孢子虫（G.plecoglossi）通过体表的微损伤被动入侵鱼类，而放射孢子（T.hovorkai，M.arcticus）则通过鳃和/或皮肤主动入侵鱼类。刀豆蛋白A处理后香鱼头肾巨噬细胞对孢子的吞噬作用明显受到抑制。当孢子用ConA处理时，吞噬作用的百分比显着降低。过氧化氢的产生被ConA处理的孢子抑制。在香鱼实验感染中，接种ConA孢子组的孢囊数量明显少于未接种孢子组。提示刀豆蛋白A能阻断香鱼巨噬细胞与多舌格孢囊蚴孢子的结合，巨噬细胞对多舌格孢囊蚴孢子的吞噬作用在多舌格孢囊蚴在香鱼体内的迁移过程中起重要作用。少