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Immunohistochemical study of enzymes using monoclonal antibodies: Oncofetal enzymes.

使用单克隆抗体对酶进行免疫组织化学研究：癌胎儿酶。

基本信息

批准号：
61480093
负责人：
YASUDA Kenjiro
金额：
$ 4.48万
依托单位：
Department of Anatomy, School of Medicine, Keio University, Shinanomach-35, Shinjuku, Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

r-GTP and GST- are known as oncofetal antigens. Since monoclonal antibodies to these enzymes are useful in diagnosis of the cancer and in the elucidation of the cartinogenic process, we have prepared monoclonal antibodies to rat, rabbit and human r-GTP of kindney, and human placenta GST- and examined the localization of these enzymes using immunohistochemistry.In adult rat kidney, r-GTP was present along the brush borders of the proximal tubules, along the basolateral membranes of the S_2 and S_3 segments of the proximal tubules and the apical cell membranes of the descending thin limbs of henle's loop. In human and rabbit kidneies, the distribution patterns of r-GTP almost coincided with those of rat. r-GTP was recognized in proximal tubules at 18-days of gestation in rat kidney. The intensity of the immunostaining increased with the develpoment of animals. The localization pattern was almost the same as that of adult. In rat liver, strong reaction was recognized along the blie canaliculi and apical membrane of interlobular bile ductal cells in neonate(0-1 days of birbh) and in partial hepatectomized rat, however, very faint reaction was present in adult rat. In human liver, immunoreaction was seen along the bile canaliculi from 7-weeks of gestation to adult: the strongest reaction was recognized at the prenatal stages. These results suggested that r-GTP is a marker for proliferating liver cells, i.e. oncofetal enzyme in liver. However, it did not show such change of distribution pattern in kidney and pancreas.GST- was localized in the amorphous cytoplasm of syncytiotrophblasts and X-cells both of which are fetal origin, in full-term human placenta.The monoclonal antibodies in this study may provide a good tool for oncological research of the enzymes in maligant tissues.

r-GTP 和 GST- 被称为癌胎儿抗原。由于针对这些酶的单克隆抗体可用于诊断癌症和阐明致癌过程，因此我们制备了针对大鼠、兔和人肾 r-GTP 以及人胎盘 GST- 的单克隆抗体，并使用免疫组织化学检查了这些酶的定位。在成年大鼠肾脏中，r-GTP 沿肾刷状缘存在。近曲小管，沿近曲小管 S_2 和 S_3 段的基底外侧膜以及亨勒袢降细肢的顶端细胞膜。在人和兔肾脏中，r-GTP的分布模式与大鼠的分布模式几乎一致。 r-GTP 在妊娠 18 天的大鼠肾脏近端小管中被识别。免疫染色的强度随着动物的发育而增加。定位模式与成人几乎相同。在大鼠肝脏中，新生大鼠（出生后0-1天）和部分肝切除大鼠的小叶间胆管细胞和小叶间胆管细胞顶膜有强烈反应，但成年大鼠的反应非常微弱。在人类肝脏中，从妊娠 7 周到成年，沿着胆小管观察到免疫反应：在产前阶段发现了最强的反应。这些结果表明r-GTP是肝细胞增殖的标志物，即肝脏中的癌胎酶。然而，在肾脏和胰腺中并未显示出这种分布模式的变化。在足月人胎盘中，GST-定位于胎儿来源的合体滋养层和X细胞的无定形细胞质中。本研究中的单克隆抗体可能为恶性组织中酶的肿瘤学研究提供良好的工具。