Molecular biologocal studies on the regulation of neuropeptide synthesis by dopamine

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Regulation of the neuropeptide synthesis by dopamine is thought to be initiated by the stimulation of the receptor,and as a result of various intracellular biochemical events,the change in the translation rate of the neuropeptide gene is observed. Althogh details of these mechanisms are unknown, second messenger systems such as cyclic nucleotides and phosphatidylinositol turnover have been reported to be involved in the regulation. In the present study, following results were obtained on the molecular structures and tissue distributions of several protein kinases which seem to play important roles in the these second messenger systems.1. We obtained the cDNA clone which contains the entire coding regoin of the type I regulatory subnit of syclic AMP-dependent protein kinase from a rat brain cDNA library by a homology proding method based on the amino acid sequence was deduced by the nucleotide sequence of the cDNA clone. Molecular weight of the subunit was determined by the homology with other cyclic nucleotide binding proteins.2. The presence of various subtypes of protein kinase C has been shown by molecular cloning techniques. Gamma<gamma>subtype of the protein kinase C mainly localizans in the central nervous system, and Purkinje cells in the cerebellum and pyramidal cells in the hippocampus showed strong immunoreactivities. Furthermore, <beta>_1 and <beta>_2 subtypes which are produced by alternative splicing mechanism also showed specific distinct localization in the neurons and nerve terminals. These results indicate that different subtypes of protein kinase C may be crrelated with different neuronal functions.