ANALYSIS OF B CELL ACTIVATION AND DIFFERENTIATION IN PRIMARY HYPOGAMMAGLOBULINEMIA.

原发性低丙种球蛋白血症中 B 细胞活化和分化的分析。

• 批准号: 61480213
• 负责人: MATSUMOTO Shuzo
• 金额: $ 2.62万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480213/
• 关键词: Primary hypogammaglobulinemia; SAC; IL-2; EBV; pre B cell; μ鎖mRNA; B細胞; CVI; Ig産生能; recombinant IL-2

The activation and differentation of B cells in 13 patients with primary hypogammaglobulinemia were studied by using EBV induced lymphoblastoic cell lines (LCL) and stimulation with SAC and SAC and SAC+recombinant IL-2 (rIL-2). DNA rearrangement and mRNA were also studied in a case with the LCL of pre B cell phenotype.I. LCL were established from peripheral blood lymphocytes with EBV in 7 cases, and the LCL from 6 cases produced predominantly IgM.II. B cells from 7 patients were tested for their ability to proliferate and differentiate under stimulation of SAC and SAC+rIL-2. They showed three different types as follows: (1)no proliferation under stimulation of SAC or SAC+rIL-2, (2) normal proliferation to SAC but not to SAC+rIL-2, (3)normal proliferation to SAC and SAC+rLL-2, but no differentation to Ig secretory cells.III. In the LCL of pre B cell phenotype, mRNA of <mu> chain showed to be short in either membranous of secretory types.

使用EBV诱导的淋巴母细胞系（LCL）研究了13例原发性低磁性血症患者B细胞的激活和分化，并用SAC和SAC和SAC+重组IL-2（RIL-2）刺激。在使用B细胞表型的LCL的情况下，还研究了DNA重排和mRNA。 LCL是在7例中用EBV的外周血淋巴细胞建立的，从6例产生的LCL主要是IGM.II。在SAC和SAC+RIL-2刺激下，对7例患者的B细胞进行了增殖和分化的能力。他们显示了三种不同类型的类型：（1）在SAC或SAC+RIL-2刺激下没有增生，（2）对SAC的正常增殖，但对SAC+RIL-2，（3）对SAC和SAC+RLL-2的正常增殖，但与IG分泌细胞没有区别。在B细胞表型的LCL中，<mu>链的mRNA在分泌类型的任何一种膜中都很短。

项目成果

松本脩三: 小児科診療. 50. 745-752 (1987)

Shuzo Matsumoto：儿科实践。50。745-752（1987）

Yukio Fukuyama: Brain & Development. 9. 270-282 (1987)

福山由纪夫：大脑

Yukio Sakiyama: "Primary Immunodeficiency Diseases. Evaluation of immunoregulatory activity in common variable immunodeficiency by use of staphylococcus aureus cowan 1." Elsevier Science Publishers B. V., 4 (1986)

Yukio Sakiyama：“原发性免疫缺陷疾病。利用金黄色葡萄球菌 cowan 1 评估常见变异型免疫缺陷的免疫调节活性。”

Hiroshi Hayakawa: "The Nature, Cellular a Biochemical Basis and Management of Immunodeficiencies. Genetically Determined Immunodeficiency" Shattauer Verlag Stuttgart, 17 (1987)

Hiroshi Hayakawa：“自然、细胞、生化基础和免疫缺陷的管理。遗传决定的免疫缺陷”Shattauer Verlag Stuttgart，17 (1987)

Yukio Sakiyama: "Abnormalities of lymphokines in immunodeficiency diseases." Journal of Clinical Science.23. 127-133 (1987)

Yukio Sakiyama：“免疫缺陷疾病中淋巴因子的异常。”