Studies on Contact Sensitivity by Using Liposome.

通过使用脂质体作为接触敏感性的人工抗原，我们已经证明，半抗原修饰的分子和Ia抗原在脂质体表面上的排列对于接触敏感性中的T细胞活化是必需的。进一步研究抗原与T细胞的相互作用。试图建立接触敏感性的T细胞克隆。用半抗原结合的表皮细胞或半抗原结合的脾细胞作为抗原刺激接触致敏小鼠的淋巴结细胞进行培养。通过用半抗原缀合的脾细胞培养淋巴结细胞来建立T细胞克隆。它们通过用半抗原缀合的脾细胞刺激而增殖，但除了一个克隆3-3克隆之外，不能将接触敏感性转移到幼稚小鼠。3-3克隆在作为抗原呈递细胞的巨噬细胞存在下与脂质体一起培养。3-3该克隆对脂质体反应良好，但在没有巨噬细胞的情况下以及用脂质体和白细胞介素1或表皮胸腺细胞活化因子（ETAF）刺激时，该克隆对脂质体没有反应。在我们以前的研究中，从淋巴结细胞中纯化的T细胞对ETAF存在下的脂质体有反应。需要进一步的研究来阐明刺激T细胞克隆的必要因素。

By using liposomes as an artificial antigen of contact sensitivity we have demonstrated that arrangement of both hapten modified molecules and Ia antigens on the surface of the liposome is essential for T cell activation in contact sensitivity. To investigate further the interaction between the antigen and T cell. it is attempted to establish T cell clones of contact sensitivity. Lymphnode cells of contact sensitized mice were cultured with stimulation of either hapten conjugated epidermal cells or hapten conjugated spleen cels as antigens. T cell clones were establi- shed by culturing lymphnode cells with hapten conjugated spleen cells. They were proliferated by stimulation with hapten conjugated spleen cells, but could not transfer contact sensitivity to naive mice except one clone, 3-3 clone. 3-3 clone was cultured with the liposome in the presence of macrophage as the antigen presenting cell. 3-3 clone responded well to the liposome but it did not respond to the liposome in the absence of macrophage and the stimulation with the liposome and interleukin 1 or epidermal thymocyte activating factor (ETAF). T cels purified from the lymphnode cells responded to the liposome in the presence of ETAF in our previous study. Further study should be indicated to elucidate the essential factors to stimulate T cell clones.