Studies on Contact Sensitivity by Using Liposome.

使用脂质体研究接触敏感性。

• 批准号: 61480228
• 负责人: NISHIKO Kiyoshi
• 金额: $ 2.82万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480228/
• 关键词: Contact Sensitivity; Liposome; T cell clone; インターロイキン1; ETAF; リポソーム; Ia抗原; 抗原認識機構; γインターフェロン

By using liposomes as an artificial antigen of contact sensitivity we have demonstrated that arrangement of both hapten modified molecules and Ia antigens on the surface of the liposome is essential for T cell activation in contact sensitivity. To investigate further the interaction between the antigen and T cell. it is attempted to establish T cell clones of contact sensitivity. Lymphnode cells of contact sensitized mice were cultured with stimulation of either hapten conjugated epidermal cells or hapten conjugated spleen cels as antigens. T cell clones were establi- shed by culturing lymphnode cells with hapten conjugated spleen cells. They were proliferated by stimulation with hapten conjugated spleen cells, but could not transfer contact sensitivity to naive mice except one clone, 3-3 clone. 3-3 clone was cultured with the liposome in the presence of macrophage as the antigen presenting cell. 3-3 clone responded well to the liposome but it did not respond to the liposome in the absence of macrophage and the stimulation with the liposome and interleukin 1 or epidermal thymocyte activating factor (ETAF). T cels purified from the lymphnode cells responded to the liposome in the presence of ETAF in our previous study. Further study should be indicated to elucidate the essential factors to stimulate T cell clones.

通过使用脂质体作为接触敏感性的人工抗原，我们证明了半抗原修饰分子和 Ia 抗原在脂质体表面的排列对于接触敏感性的 T 细胞激活至关重要。进一步研究抗原与T细胞之间的相互作用。试图建立接触敏感性的T细胞克隆。通过半抗原缀合的表皮细胞或半抗原缀合的脾细胞作为抗原的刺激来培养接触致敏小鼠的淋巴结细胞。通过将淋巴结细胞与半抗原结合的脾细胞一起培养来建立T细胞克隆。它们通过半抗原缀合的脾细胞的刺激而增殖，但除了1个克隆、3-3个克隆外，不能将接触敏感性转移至初始小鼠。在巨噬细胞作为抗原呈递细胞存在的情况下，用脂质体培养3-3克隆。 3-3克隆对脂质体反应良好，但在没有巨噬细胞以及用脂质体和白细胞介素1或表皮胸腺细胞激活因子(ETAF)刺激的情况下，它对脂质体没有反应。在我们之前的研究中，在 ETAF 存在的情况下，从淋巴结细胞纯化的 T 细胞对脂质体有反应。需要进一步研究来阐明刺激 T 细胞克隆的基本因素。

项目成果

西岡清,片山一朗,小林興市,滝尻玲重: J.Devmatology. 13. 226-227 (1986)

Kiyoshi Nishioka、Ichiro Katayama、Koichi Kobayashi、Rei Takijiri：J.Devmatology。 13. 226-227 (1986)

西岡清: Medial Immunology. 11. 99-103 (1986)

西冈清：医学免疫学 11. 99-103 (1986)

Kiyoshi.: "Contact dermatitis." Shonikashinryou. 50. 2045-2047 (1987)

清志：“接触性皮炎。”

西岡清: 日本皮膚科学会雑誌. 97. 1337-1338 (1987)

Kiyoshi Nishioka：日本皮肤病学会杂志 97. 1337-1338 (1987)。

Katayama, Ichiro and Nishioka, Kiyoshi.: "Systemic contact dermatitis medicamentosa induced by topical eye lotion in a patient with corneal allograft." Archives of Dermatology. 123. 436-437 (1987)

Katayama, Ichiro 和 Nishioka, Kiyoshi.：“角膜同种异体移植患者局部眼药水诱发的系统性接触性药物性皮炎。”