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The Study of Cardiac Lymph; Special Reference to Cardiac Surgery

心脏淋巴的研究；

基本信息

批准号：
61480297
负责人：
TAIRA Akira
金额：
$ 4.16万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The experiment has been attempted to clarify the relationship between dynamical aspect of cardiac lymph and the extent of myocardial ischemic damage. For this purpose, the left anterior descending coronary artery of the mongrel dog was occluded and cardiac lymph under such a condition was collected at the cardiac lymph node. Flow volume and component of the cardiac lymph were studied at fixed intervals of time. The result clarified that the cardiac lymph with an occluded coronary artery revealed augmentation of the flow. Enzymes concentration (GOT, GPT, LDH, etc.) in the cardiac lymph originated from ischemic area of the myocardium were strikingly high in comparison with those in the serum. Both phenomena of increased lymph flow and augmented enzymes level were further aggravated in a phase of reperfusion with reactive hyperemia. An extent of the infarct area was well correlated to the increased volume of the cardiac lymph.The next experiment with administration of hyaluronidase intending an active remove of cardiac lymph from ischemic area revealed a prominent effect in reduction of the infarct area. The risk area (R) for infarction was defined as the area accorded with tributary of the ligated coronary artery. The infarct area (I) was determined as an extent where TTC dyeing appeared in negative. Then the ratio of I/R decreased significantly (p<0.01) after use of hyaluronidase. The values are changed from 44.1 1.1 (SE) to 9.4 4.9. Myocardial water content measured by dry/wet weight method was proportionally correlated to this result that the lesser weight to the smaller I/R value. Further study in this experimental series is directing to the relationship between myocardial lymphstasis and the extent of myocardial infarction. The area of infarction apparently increased under myocardial lymph stasis.

本实验旨在阐明心肌淋巴液动力学变化与心肌缺血损伤程度的关系。为此，闭塞杂种狗的冠状动脉左前降支，并在心脏淋巴结处收集这种条件下的心脏淋巴液。以固定的时间间隔研究心脏淋巴液的流量和成分。结果表明，冠状动脉闭塞的心脏淋巴液显示流量增加。酶浓度（GOT、GPT、LDH等）与血清相比，心肌缺血区心肌淋巴液中的TNF-α明显升高。淋巴流量增加和酶水平升高的现象在反应性充血的再灌注阶段进一步加重。梗死面积的范围与心脏淋巴液体积的增加密切相关。接下来的实验中，给予透明质酸酶，旨在从缺血区域主动清除心脏淋巴液，显示出显著的缩小梗死面积的效果。梗死危险区域（R）定义为与结扎的冠状动脉分支相一致的区域。梗塞面积（I）被确定为TTC染色呈阴性的程度。应用透明质酸酶后，I/R比值明显降低（P<0.01）。数值从44.1 1.1（SE）变更为9.4 4.9。干/湿重法测定的心肌含水量与I/R值越小的心肌重量成正比。本实验系列的进一步研究旨在探讨心肌淋巴结郁积与心肌梗死范围的关系。心肌淋巴结瘀滞时梗死面积明显增大。