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A study of simultaneous multi-organ transplantaion after hypothemic preservation using abdominal cavity cooling

腹腔低温保存后同步多器官移植的研究

基本信息

批准号：
05454357
负责人：
TAIRA Akira
金额：
$ 4.29万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

General mild hypothermia of 32ﾟC for multi-organ preservation in Tx was introduced in pigs by means of ice slush use in the abdominal cavity. In our previous study the method provides an ideal hypothermia for all organs to be transplanted.The experiment was divided into thtree parts.The first was the study of brain death and abdominal cavity cooling, in which the use of 'amurinone' provided an excellent hemodynamic condition by means of a favorable right heart work with low pulmonary vascular resistance. The second experiment was the observation of multi-organs viability during and after introduction of this preservation to the animals. In this series, the target organs in abdominal cavty preservation were the liver, the pancreas, and the gut and the heart. Succeeding orthotopic transplantaion was done on the heart as the representative organ. The result was satisfactory with a shorter period of recovery from anerobic condition during preservation. No particular differences were observed among the data of abdominal multi-organs after preservation. The third experiment was deal with an enbloc transplantation of the abdominal multiorgans followed by tissue blood flow measurement in each preserved and transplanted organ together with ATP level measurement. The results are not significantly differed between organs with or without hypothermic preservation. The experiment clarified that the abdominal cavity cooling is a useful tool in preservation of multi-organs to be transplanted. In practice, it has a protective effect in an urgent and unexpected ischemic conditions of organs during the course of preservation.

通过在猪腹腔内使用冰沙，在Tx中引入用于多器官保存的32 ° C的一般亚低温。在我们以前的研究中，该方法为所有待移植器官提供了理想的低温。实验分为三个部分：第一部分是脑死亡和腹腔冷却的研究，其中使用“amurinone”通过有利的右心工作和低肺血管阻力提供了良好的血液动力学条件。第二个实验是在将这种保存方法引入动物期间和之后观察多器官活力。在本系列中，腹腔保留的靶器官是肝脏、胰腺、肠道和心脏。以心脏为代表器官进行原位移植。结果是令人满意的，从厌氧条件下保存期间的恢复时间较短。保存后的腹部多器官数据之间没有观察到特别的差异。实验三为腹部多器官整体移植，测定各器官保存和移植后组织血流量及ATP水平。低温保存与非低温保存的结果无显著差异。实验表明，腹腔冷却是一种有效的保存多器官移植的工具。在实践中，它在保存过程中器官的紧急和意外缺血条件下具有保护作用。