A study of simultaneous multi-organ transplantaion after hypothemic preservation using abdominal cavity cooling

采用32ﾟC全身亚低温保存猪TX多脏器，采用冰冻灌肠的方法。在我们以前的研究中，这种方法为所有器官的移植提供了理想的低温。实验分为三个部分。第一个是脑死亡和腹腔降温的研究，其中‘氨尿酮’的使用通过良好的右心工作和低肺血管阻力提供了良好的血流动力学条件。第二个实验是观察动物在引入这种保存过程中和保存后多器官的存活情况。在本系列中，保留腹腔的靶器官是肝脏、胰腺、肠道和心脏。随后以心脏为代表器官进行原位移植。结果令人满意，保存期从厌氧状态恢复的时间较短。腹部多脏器保存后的数据差异无统计学意义。第三个实验是腹部多器官整体移植，然后测量每个保存和移植器官的组织血流量，并测量ATP水平。结果在低温保存和不低温保存的器官之间没有显著差异。实验表明，腹腔降温是保存多器官移植的一种有效手段。在实践中，在器官保存过程中，对紧急和意外的器官缺血情况具有保护作用。

General mild hypothermia of 32ﾟC for multi-organ preservation in Tx was introduced in pigs by means of ice slush use in the abdominal cavity. In our previous study the method provides an ideal hypothermia for all organs to be transplanted.The experiment was divided into thtree parts.The first was the study of brain death and abdominal cavity cooling, in which the use of 'amurinone' provided an excellent hemodynamic condition by means of a favorable right heart work with low pulmonary vascular resistance. The second experiment was the observation of multi-organs viability during and after introduction of this preservation to the animals. In this series, the target organs in abdominal cavty preservation were the liver, the pancreas, and the gut and the heart. Succeeding orthotopic transplantaion was done on the heart as the representative organ. The result was satisfactory with a shorter period of recovery from anerobic condition during preservation. No particular differences were observed among the data of abdominal multi-organs after preservation. The third experiment was deal with an enbloc transplantation of the abdominal multiorgans followed by tissue blood flow measurement in each preserved and transplanted organ together with ATP level measurement. The results are not significantly differed between organs with or without hypothermic preservation. The experiment clarified that the abdominal cavity cooling is a useful tool in preservation of multi-organs to be transplanted. In practice, it has a protective effect in an urgent and unexpected ischemic conditions of organs during the course of preservation.