A study of simultaneous multi-organ transplantaion after hypothemic preservation using abdominal cavity cooling

采用冰浆在猪腹腔内应用32℃亚低温法进行多器官保存。在我们之前的研究中，该方法为所有移植器官提供了理想的低温。实验分为三个部分。第一个是脑死亡和腹腔冷却的研究，其中使用“氨脲酮”通过有利的右心功和低肺血管阻力提供了良好的血液动力学条件。第二个实验是观察这种保存方法引入动物期间和之后的多器官活力。在这个系列中，腹腔保存的目标器官是肝脏、胰腺、肠道和心脏。随后以心脏为代表器官进行了原位移植。结果令人满意，保存期间从缺氧状态恢复的时间较短。保存后各腹部多脏器数据无明显差异。第三个实验是对腹部多器官进行包体移植，然后测量每个保存和移植器官的组织血流并测量ATP水平。低温保存与不低温保存的结果无显著差异。实验结果表明，腹腔冷却是保存待移植多器官的有效手段。在实践中，它对保存过程中器官的紧急和意外缺血情况具有保护作用。

General mild hypothermia of 32ﾟC for multi-organ preservation in Tx was introduced in pigs by means of ice slush use in the abdominal cavity. In our previous study the method provides an ideal hypothermia for all organs to be transplanted.The experiment was divided into thtree parts.The first was the study of brain death and abdominal cavity cooling, in which the use of 'amurinone' provided an excellent hemodynamic condition by means of a favorable right heart work with low pulmonary vascular resistance. The second experiment was the observation of multi-organs viability during and after introduction of this preservation to the animals. In this series, the target organs in abdominal cavty preservation were the liver, the pancreas, and the gut and the heart. Succeeding orthotopic transplantaion was done on the heart as the representative organ. The result was satisfactory with a shorter period of recovery from anerobic condition during preservation. No particular differences were observed among the data of abdominal multi-organs after preservation. The third experiment was deal with an enbloc transplantation of the abdominal multiorgans followed by tissue blood flow measurement in each preserved and transplanted organ together with ATP level measurement. The results are not significantly differed between organs with or without hypothermic preservation. The experiment clarified that the abdominal cavity cooling is a useful tool in preservation of multi-organs to be transplanted. In practice, it has a protective effect in an urgent and unexpected ischemic conditions of organs during the course of preservation.