Studies on pathophysiology and treatment of cerebral hypoxia with special reference to blood brain barrier

特别针对血脑屏障的脑缺氧的病理生理学和治疗研究

• 批准号: 61480330
• 负责人: ITO Yusuke
• 金额: $ 2.24万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480330/
• 关键词: cerebral hypoxia; blood brain harrer; methylpredonisolone; urinastatin; neuronal nucleus; striatum; catecholamine; Ca channel blocker; 脳虚血; 神経細胞核; 形状計測; 塩酸フルナリジン; 脳蘇生

1. The beneficial effect of methylpredonisolone, urinastatin, vitamin E and coenzyne Q on membrane stability was investigated in rat brains induced by an infusion of hypertonic mannitol solution via the carotid artery.It was found the pretreatment of methylpredonisolone and urinastatin significantly inhibited Evans blue strainly. But no significant difference was observed by vitamin E and coenzyne Q.2. The concentrations of catecolamins and their metabolites (dopamine, HVA, DOPAC, norepinephrine) in striatum was measured during 5% oxygen inhalation and subsequent recovery period in rats, Flunarizine, a calcium channel blocker, was administered and its effect on catecholamine fluctuations was studied. Dopamine increased with 5% oxygen inhalation. DOPAC and norepinephrine decreased and no changes were seen in HVA. After 20 min of hypoxia, catecholamine contents returned to the control levels in another 2 hours of air breathing.When flunarizine was administered before hand. Changes in con … More tent appeared to be lower during hypoxia and subsequent recovery period.3. The morphological changes and calcium accumulation were studied in rat pyramidal nerve cell which had undergone 5% oxygen inhalation and bilateral carotid artery clampings for 15 minutes, using electron and X-ray microanalytical microscopes. To study the protective effects of nicardipine on brain hypoxia, the same techniques were used after hypoxic event. In the 5% oxygen inhalated group, the chromatinclamping in the nucleus was observed, but calcium ions could not be detected in the neuron. In the carotid clamping group under 5% oxygen inhalation, severe nuclear change and mitochondrial swelling were observed and calcium ions were detected in the mitochondrion. After administration of nicardipine under hypoxia and under carotid clamping with hypoxia, similar morphological changes were recognized. It is, therefore, concluded that notable protective effects of nicardipine on ischemic hypoxia were not obtained in this morphological experiment. Less

1. 研究了甲基强的松龙、尿司他丁、维生素E和辅酶Q对经颈动脉输注高渗甘露醇溶液诱导的大鼠脑膜稳定性的影响。发现甲基强的松龙和尿司他丁预处理对Evans蓝菌有明显抑制作用。维生素E和辅酶q2含量差异不显著。测定大鼠纹状体中儿茶酚胺及其代谢物（多巴胺、HVA、DOPAC、去甲肾上腺素）在5%吸氧及恢复期的浓度，并观察钙通道阻滞剂氟氟利嗪对儿茶酚胺波动的影响。多巴胺随吸氧5%增加。多巴胺和去甲肾上腺素降低，HVA无明显变化。缺氧20分钟后，儿茶酚胺含量在另外2小时的空气呼吸中恢复到控制水平。当氟桂利嗪在手术前使用时。在缺氧和随后的恢复期，更多的帐篷出现较低的变化。采用电子显微镜和x射线显微分析技术研究了5%氧吸入和双侧颈动脉夹持15 min后大鼠锥体神经细胞的形态变化和钙积累情况。为了研究尼卡地平对脑缺氧的保护作用，在缺氧事件后采用相同的技术。5%氧吸入组细胞核内可见染色质嵌套，但神经元内未见钙离子。颈动脉钳夹组在5%吸氧条件下，观察到严重的核改变和线粒体肿胀，线粒体中检测到钙离子。在缺氧和颈动脉缺氧夹持下给予尼卡地平后，发现相似的形态学改变。因此，本形态学实验未获得尼卡地平对缺血性缺氧的显著保护作用。少

项目成果

伊藤祐輔: "酸欠症の病態" 日本医事新報. No.3322. 127-128 (1987)

伊藤佑介：“氧缺乏的病理学”日本医学报纸第3322. 127-128（1987）。

伊藤祐輔: "現代麻酔科学(救急蘇生法)" 朝倉書店, 12 (1987)

伊藤佑介：《现代麻醉学（紧急复苏）》朝仓书店，12（1987）

矢作直樹: 麻酔. 35. 1176-1180 (1986)

矢萩直树：《麻醉》35。1176-1180 (1986)

山崎光章: "5%酸素吸入によるラット線条体カテコラミン含量の変動とフルナリジンの効果" 麻酔. 38. 312-319 (1989)

Mitsuaki Yamazaki：“吸入 5% 氧气引起的大鼠纹状体儿茶酚胺含量的变化和氟桂利嗪的影响”麻醉 38. 312-319 (1989)。

山崎光章: 麻酔. 38. 312-319 (1989)

山崎光明：麻醉。38. 312-319 (1989)