Studies on pathophysiology and treatment of cerebral hypoxia with special reference to blood brain barrier

1.本文观察了甲基强的松龙、乌司他丁、维生素E和辅酶Q对经颈动脉注入高渗甘露醇所致大鼠脑细胞膜稳定性的影响，发现甲基强的松龙和乌司他丁预处理可显著抑制伊文思蓝的产生。而维生素E和辅酶Q则无显著性差异。测定大鼠吸入5%氧后及恢复期纹状体内儿茶酚胺及其代谢产物多巴胺、HVA、DOPAC、去甲肾上腺素的浓度，并观察氟桂利嗪对儿茶酚胺波动的影响。多巴胺增加5%的氧气吸入。DOPAC和去甲肾上腺素降低，HVA无变化。缺氧20min后，再呼吸空气2h，脑组织中儿茶酚胺含量恢复到对照组水平。变更内容 关于我们 在缺氧和随后的恢复期，tent出现降低。本文用电子显微镜和X射线显微分析显微镜观察了大鼠双侧颈总动脉夹闭和吸入5%氧15分钟后锥体神经细胞的形态学变化和钙离子的积聚。为研究尼卡地平对脑缺氧的保护作用，采用缺氧后同样的方法。5%氧吸入组细胞核染色质夹闭，但未检测到钙离子。在吸入5%氧的条件下，颈动脉钳夹组可见严重的核改变和线粒体肿胀，线粒体内可见钙离子。在缺氧和缺氧条件下给予尼卡地平后，识别出类似的形态学变化。因此，可以得出结论，在本形态学实验中，尼卡地平对缺血缺氧没有显著的保护作用。少

1. The beneficial effect of methylpredonisolone, urinastatin, vitamin E and coenzyne Q on membrane stability was investigated in rat brains induced by an infusion of hypertonic mannitol solution via the carotid artery.It was found the pretreatment of methylpredonisolone and urinastatin significantly inhibited Evans blue strainly. But no significant difference was observed by vitamin E and coenzyne Q.2. The concentrations of catecolamins and their metabolites (dopamine, HVA, DOPAC, norepinephrine) in striatum was measured during 5% oxygen inhalation and subsequent recovery period in rats, Flunarizine, a calcium channel blocker, was administered and its effect on catecholamine fluctuations was studied. Dopamine increased with 5% oxygen inhalation. DOPAC and norepinephrine decreased and no changes were seen in HVA. After 20 min of hypoxia, catecholamine contents returned to the control levels in another 2 hours of air breathing.When flunarizine was administered before hand. Changes in con … More tent appeared to be lower during hypoxia and subsequent recovery period.3. The morphological changes and calcium accumulation were studied in rat pyramidal nerve cell which had undergone 5% oxygen inhalation and bilateral carotid artery clampings for 15 minutes, using electron and X-ray microanalytical microscopes. To study the protective effects of nicardipine on brain hypoxia, the same techniques were used after hypoxic event. In the 5% oxygen inhalated group, the chromatinclamping in the nucleus was observed, but calcium ions could not be detected in the neuron. In the carotid clamping group under 5% oxygen inhalation, severe nuclear change and mitochondrial swelling were observed and calcium ions were detected in the mitochondrion. After administration of nicardipine under hypoxia and under carotid clamping with hypoxia, similar morphological changes were recognized. It is, therefore, concluded that notable protective effects of nicardipine on ischemic hypoxia were not obtained in this morphological experiment. Less