A Study of Cellular Communication on Malignant Progression in Urogenital Tumors

用染料转移法研究了人泌尿生殖道上皮细胞间通讯（IC）。1)在移行细胞癌（TCC）细胞中，分化和未分化的TCC细胞中分别观察到高和低IC。在不同TCC细胞的各种组合之间观察到高IC或IC缺乏。2）前列腺上皮中，前列腺癌细胞间的IC程度低于良性前列腺疾病。从骨转移灶（PC-3）和淋巴结中培养的前列腺癌细胞IC非常有限。良性前列腺上皮与PC-3之间几乎不存在IC。前列腺上皮细胞与间质细胞间无IC存在。这种抑制是时间和剂量依赖性的，并迅速恢复后剥夺睾酮。相反，雌二醇-17和皮质醇均不抑制这些TCC细胞的IC。成纤维细胞的IC不受睾酮的影响。4）检测细胞角蛋白和细胞表面抗原以检测尿路上皮的恶性转化。(1)在正常尿路上皮中，角蛋白纤维细而直，直径恒定，均匀分布于细胞质中。在TCC细胞中，它们直径不同，蜿蜒穿过，并且在细胞质中分布不均匀。膀胱癌细胞中Ketatin纤维的变化与肿瘤的恶性程度呈平行关系。5）三种抗膀胱癌细胞株（JTC-30）的单克隆抗体（MoAb）均与A型抗原相关，但与抗A型单克隆抗体的反应模式不同。这些抗JTC-30 MoAb识别血型A相关表位，其在恶性变性过程中不稳定。

Intercellular communication (IC) was investigated by dye transfer method in the human urogenital epithelia. 1) In transitional cell carcinoma (TCC) cells, high and low IC was observed in differenciated and undifferenciated TCCs, respectively. High or lack of IC was observed between various combination of different TCC cells. Fibroblasts showed apparent IC with none of TCC cells.2) In prostatic epithelium, the extent of IC among prostatic carcinoma cells was lower than that of benign prostatic diseases. IC of prostatic carcinoma cells cultured from metastatic lesions of bone (PC-3) and lymph nodes was very limited. IC between benign prostatic epithelium and PC-3 was almost absent. No IC was present between prostatic epithelium and interstitial cells.3) Testosterone inhibites IC of TCC cell lines. This inhibition was time- and dose-dependent and recovered rapidly after deprivation of testosterone. In contrast, neither estradiol-17 nor cortisol inhibites IC of these TCC cells. IC of fibroblasts was not influenced by testosterone.4) To detect malignant transformation of the urothelium, cytokeration and cell surface antigens were investigated. (1) In normal urothelium, keratin fibers are thin, straight, constant in diameter and are distributed evenly in the cytoplasm. In TCC cells, they vary in diameter, wind their ways through and are distributed unevenly in the cytoplasm. The changes of ketatin fibers in bladder carcinoma cells appeared to be parallel to the grade of malignancy.5) Three monoclonal antibodies (MoAb) generated against a TCC cell line (JTC-30) appeared to be related to blood group A antigen but have different reaction patterns with anti-blood group A MoAb. These anti-JTC-30 MoAbs recognize a blood group A related epitope(s), which is unstable in the process of malignant degeneration.