Tetracycline-labelling in decalcified bone section

脱钙骨切片中的四环素标记

• 批准号: 61480375
• 负责人: YOSHIKI Shusaku
• 金额: $ 2.56万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480375/
• 关键词: Hard tissues; Decalcified sections; TC標識

Evidence of tetracycline(TC)-labelling in decalcified dentin was reported and TC uptake by a part of the organic component in calcified tissues was reconfirmed ( Simpson, 1981). This report deals with an experimental study to know possible demonstration of TC-labelling in decalcifed bone section. The results were as follows: (1) About 5 week-immersion fixation of bone tissues by 10% neutral formalin was necessary for possile demonstration of TC-labelling in decalcifed bone sections. (2) Additional fixation by cyanuric chloride after formalin fixation showed little effect for the preservation of TC-labelling in the sections. (3) Decalcification by neutral EDTA must be applied for the same purpose. (4) Degree of TC-labelling demonstrated in decalcified bone sections was varied with a type of administrated tetracycline.

报告了脱钙牙本质中四环素（TC）标记的证据，并再次证实了钙化组织中部分有机成分对TC的摄取（Simpson，1981）。本文报道了一项实验研究，以了解脱钙骨切片中TC标记的可能性。结果如下：（1）在脱钙骨切片中，TC标记的可能性需要用10%中性福尔马林浸泡固定5周左右。(2)福尔马林固定后，通过三聚氯氰的额外固定对保存切片中的TC标记几乎没有影响。(3)中性EDTA脱钙必须用于相同目的。(4)脱钙骨切片中TC标记的程度随施用的四环素类型而变化。

项目成果

山崎亨, 中村雄一, 立川哲彦, 吉木周作: 昭和歯学学会雑誌. 8. (1988)

Toru Yamazaki、Yuichi Nakamura、Tetsuhiko Tachikawa、Shusaku Yoshiki：昭和牙科学会杂志 8。（1988）。

T.Yamazaki, Y.Nakamura, T.Tachikawa and S.Yoshiki: "Experimental study of tetracycline-labelling in decalcified bone section" J. Showa Univ. Dent. Soc.8. (1988)

T.Yamazaki、Y.Nakamura、T.Tachikawa 和 S.Yoshiki：“脱钙骨切片中四环素标记的实验研究”J. Showa Univ。

S. Yohiki;T. Tachikawa;T. Yamazaki;Y. Nakamura: Journal of Dental Research(Divisional abstract). 66. 921 (1987)

S.洋彦；T.

S.Yoshiki, T.Tachikawa, T.Yamazaki and Y.Nakamura: Nishimura , Niigata. Bone Morphometry, (1988)

S.Yoshiki、T.Tachikawa、T.Yamazaki 和 Y.Nakamura：新泻西村。

吉木周作, 立川哲彦, 山崎亨, 中村雄一: "骨形態計測第8巻" 西村書店, (1988)

Shusaku Yoshiki、Tetsuhiko Tachikawa、Toru Yamazaki、Yuichi Nakamura：《骨形态测量第 8 卷》西村书店，(1988)