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Study of the mechanism of bone formation using morphological and functional analyzes

利用形态学和功能分析研究骨形成机制

基本信息

批准号：
05304042
负责人：
YOSHIKI Shusaku
金额：
$ 7.36万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304042/
关键词：
bone formation osteoblast chondrocyte osteoclast osteocyte BMP in situ hybridization cell culture in situ hybridization In situ hybridization

项目摘要

The mechanism involved in bone formation has been investigated using morphological, biochemical and molecular biological techniques, and the following results are obtained.1.The important roles of BMP-2 in the differentiation process of osteoblasts has been demonstrated using various kinds of cell lines (Yoshiki S., et al.).2.The age-related decreases in mRNA expression for bone matrix proteins in osteoblasts were revealed by in situ hybridization(Yoshiki S., et al.).3.Several genes for BMPs and BMP receptors were cloned. The changes in mRNA expression for Hox genes were analyzed during the process of ectopic bone formation induced by BMP(Sasaki S., et al.).4.BMP-2 inhibited myogenic differentiation in myoblasts, and converted their differentiation pathway into osteoblast cell lineage (Suda T., et al.).5.The tissue distribution of ChM-1 was revealed by immunohistochemistry and in situ hybridization. The important roles of ChM-2 was demonstrated in the process of bone formation and bone resorption(Suzuki F., et al.).6.The new technique for isolation of osteocytes was developed (Kumegawa M., et al.).7.Interaction among osteoblasts, osteocytes and osteoclasts were investigated using ultrastructural histochemistry and radar microscope(Ozawa H., et al).

利用形态学、生物化学和分子生物学技术研究了骨形成中涉及的机制，并获得了以下结果：1. BMP-2在成骨细胞分化过程中的重要作用已经用各种细胞系证明（Yoshiki S.，等人）。2.通过原位杂交揭示了成骨细胞中骨基质蛋白mRNA表达的年龄相关性降低（Yoshiki S.，等人）。3.克隆了几个BMP和BMP受体的基因。在BMP诱导的异位骨形成过程中分析Hox基因mRNA表达的变化（Sasaki S.，等人）。4. BMP-2抑制成肌细胞的肌源性分化，并将其分化途径转化为成骨细胞谱系（苏达T.，等）。5.用免疫组化和原位杂交方法观察ChM-1在大鼠脑组织中的分布。ChM-2在骨形成和骨吸收过程中的重要作用已得到证实（Suzuki F.，等人）。6.开发了分离骨细胞的新技术（Kumegawa M.，等）。7.使用超微结构组织化学和雷达显微镜研究成骨细胞、骨细胞和破骨细胞之间的相互作用（Ozawa H.，等）。