confocal laserscanning microskope including FLIM/FCS

共焦激光扫描显微镜，包括 FLIM/FCS

• 批准号: 437530384
• 金额: --
• 依托单位: Carl von Ossietzky Universität Oldenburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/437530384?language=en
• 关键词: confocal; laserscanning; microskope; including; FLIM

Neuronal outgrowth is regulated by different factors. The extracellular side, for example, is rich in receptor-active phospholipids. These phospholipids are able to bind to G-coupled receptors and thereby regulate multiple cellular processes such as proliferation, migration, and cell differentiation, and can act as repulsive or attractive factors on neurons. Phospholipids such as the lysophospholipids (LPA) are located in the brain in relevant concentrations and act on neurites. Until recently, only one LPA-modifying protein class, the lipid phosphate phosphatases (LPPs), was known. These proteins can regulate the LPA level on the extracellular side by LPA dephosphorylation. In 2003, we found a novel class of brain-specific LPA-modifying proteins, the plasticity-related genes (PRGs). So far, five different members have been identified and cloned. All are dynamically expressed during brain development and involved in regeneration processes by modifying LPA. Based on these findings, I am currently focusing my research on identifying the functional relevance of phospholipids in the brain and how they are controlled. Thus, we were able to identify a novel mechanism of axonal outgrowth. Furthermore, our results on the functional relevance of phospholipids suggest that they are involved in neuronal transmission processes. This mechanism has to be clarified in the future.

神经元的生长受不同因素的调节。例如，细胞外侧富含受体活性磷脂。这些磷脂能够与G偶联受体结合，从而调节多种细胞过程，如增殖、迁移和细胞分化，并可作为排斥或吸引因素对神经元起作用。溶血磷脂(LPA)等磷脂在大脑中以相应的浓度存在，并作用于神经突起。直到最近，人们只知道一类LPA修饰蛋白，即脂磷酸盐磷酸酶(LPPs)。这些蛋白质可以通过LPA去磷酸化来调节细胞外侧的LPA水平。2003年，我们发现了一类新的脑特异性LPA修饰蛋白-可塑性相关基因(PRGS)。到目前为止，已经确定并克隆了五个不同的成员。它们都在大脑发育过程中动态表达，并通过修改LPA参与再生过程。基于这些发现，我目前的研究重点是确定磷脂在大脑中的功能相关性以及它们是如何控制的。因此，我们能够确定轴突生长的一种新机制。此外，我们关于磷脂功能相关性的结果表明，它们参与了神经元的传递过程。这一机制必须在未来得到澄清。