confocal laserscanning microscope

共焦激光扫描显微镜

• 批准号: 509154085
• 金额: --
• 依托单位: Universität zu Lübeck
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/509154085?language=en
• 关键词: confocal; laserscanning; microscope

The research at the Institute of Biomedical Optics (BMO) at the University of Lübeck (UzL), focuses on new optical imaging methods, which are developed with regard to diagnostic applications.For the development of innovative light-based therapeutic and diagnostic procedures, cellular processes are becoming increasingly important. Since there is currently no possibility at the institute for routine, high-resolution imaging of subcellular dynamic processes, a modern confocal laser scanning microscope (cLSM) with 3 excitation wavelengths and 2 detection channels should be acquired.The instrument will significantly support the scientific work of six BMO research groups, external cooperation partners of the UzL and also clinical project partners at the University Medical Center Schleswig-Holstein (UKSH). On one hand, it will contribute to the development of new optical imaging techniques (Raman microscopy, SLIDE microscopy and coherence tomography or microscopy), and on the other hand, it will be used to investigate the optical manipulation of proteins and cells and the cellular mechanisms of optical diagnostic and therapeutic methods in ophthalmology. Concrete fields of application are, for example, investigations of the intracellular transport of photoactivatable agents with subcellular resolution or the investigation of functional changes of RPE cells in cell cultures after hyperthermia. The device is operated in a dedicated, centrally accessible room in the BMO building and is supervised by qualified personnel.

吕贝克大学 (UzL) 生物医学光学研究所 (BMO) 的研究重点是针对诊断应用而开发的新光学成像方法。为了开发创新的光治疗和诊断程序，细胞过程变得越来越重要。由于该研究所目前无法对亚细胞动态过程进行常规、高分辨率成像，因此应购买一台具有 3 个激发波长和 2 个检测通道的现代共焦激光扫描显微镜 (cLSM)。该仪器将极大地支持六个 BMO 研究小组、UzL 的外部合作伙伴以及石勒苏益格-荷尔斯泰因大学医学中心的临床项目合作伙伴的科学工作 （英国SH）。一方面，它将有助于新光学成像技术（拉曼显微镜、幻灯片显微镜和相干断层扫描或显微镜）的发展，另一方面，它将用于研究蛋白质和细胞的光学操纵以及眼科光学诊断和治疗方法的细胞机制。具体的应用领域是，例如，以亚细胞分辨率研究光活化剂的细胞内运输，或研究细胞培养物中热疗后RPE细胞的功能变化。该设备在 BMO 大楼内可进入的专用房间内操作，并由合格人员进行监督。