The role of genes associated with cell growth in proliferating diseases

细胞生长相关基因在增殖性疾病中的作用

• 批准号: 61480434
• 负责人: KUZUMAKI Noboru
• 金额: $ 4.74万
• 依托单位: Hokkaido University.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480434/
• 关键词: Psoriasis; oncogenes; oncogene products; monoclonal antibody; mRNA; マウス形質細胞腫; 正常復帰細胞株; 皮膚腫瘍

Expressions of several protooncogenes related with cell growth were examined in psoriatic tissues comparing those in normal skin tissues using monoclonal antibody and Northern blotting techuiques. Immunofluorescence studies using monoclonal antibody rp35 reacted with ras p21 revealed that only based layers of epidermis were stained in normal skin, while whole layers of epidermis were stained in psoriatic tissues, sugges ting overproduction of ras p21 in this disease. Northern blotting techniques with poly a(+) mRNA derived from psoriatic tissues and normal skin demonstrated that levels of fos and myc transcripts were decreased, while that of K-ras transcript was increased in psoriatic tissues in comparison with those in normal skins. All these data suggest association of protooncogenes with pathogenesis of psoriasis.

应用单克隆抗体和北方印迹技术检测银屑病组织中与细胞生长相关的几种原癌基因的表达，并与正常皮肤组织进行比较。用rasp 35单克隆抗体与rasp 21反应的免疫荧光研究显示，在正常皮肤中，只有基底层的表皮被染色，而在银屑病组织中，整个表皮层都被染色，这说明rasp 21在这种疾病中过度产生。用北方印迹技术检测银屑病患者和正常皮肤中poly a（+）mRNA，结果显示银屑病患者皮损中fos和myc基因转录水平较正常皮肤降低，而K-ras基因转录水平较正常皮肤升高。所有这些数据表明原癌基因与银屑病的发病机制。

项目成果

小木曾嘉文: 日本癌学会総会記事. 45. 518 (1986)

Yoshifumi Ogiso：日本癌症协会大会文章 45. 518 (1986)。

Kobayashi, H.: 日本研究皮膚科学会年次学術大会抄録集. 12. 111-112 (1987)

Kobayashi, H.：日本皮肤病研究学会年度科学会议摘要 12. 111-112 (1987)。

Ogiso,Y: "Expression of proto-oncogenes in normal and tumor tissues of human skin" J. Investig. Dermatol.In press.

Ogiso，Y：“原癌基因在人类皮肤正常和肿瘤组织中的表达”J. Investig。

Kuzumaki, N.: 日本研究皮膚科学会年次学術大会抄録集. 12. 99 (1987)

Kuzumaki, N.：日本皮肤病研究学会年度科学会议摘要 12. 99 (1987)。

葛巻暹: 日本癌学会総会記事. 45. 501 (1986)

Akira Kuzumaki：日本癌症协会大会文章 45. 501 (1986)