QUANTIFICATION OF ONCOGENE PRODUCTS IN CELLS AND TISSUE

这个项目的目标是概念化一种分析方法来 对细胞和组织中的癌基因产物进行定量。在第一阶段 I努力，由HER2癌基因p185 HER2编码的蛋白质将是 被用作一个具体的例子，因为它与 乳腺癌的生物学行为及其对预后的影响 意义。所使用的技术将是高分辨率的技术 数字图像显微镜和免疫组织化学定位。自.以来 免疫组织化学允许对个体细胞进行分析，非常小 可以使用组织样本，使这种分析能够在小范围内进行 细针抽吸物的病变、活检或细胞学涂片 乳房肿瘤。然而，免疫组织化学的缺点是 技术就是方式。本研究旨在改善这一点。 限制。提出了一种基于数字图像的检测方法 处理过程中，将是对肿瘤基因表达的定量分析 单个细胞可以轻松、快速地在 样本样本中的恶性细胞。

The objective of this project is to conceptualize an assay method to quantitate - oncogene products in cells and tissues. In the first Phase I efforts, the protein encoded by the HER2 oncogene, P185 HER2, will be used as a specific' example, since it has been strongly implicated in the biological behavior of breast cancer and may have prognostic significance. The techniques used will be those of high resolution digital image microscopy and immunohistochemical localization. Since immunohistochemistry permits analysis of individula cells, very small tissue samples can be used, enabling this assay to be performed on small lesions, biopsies or cytological smears of fine needle aspirates of breast tumors. However the disadvantage of immunohistochemical techniques is the manner. This research is aimed at improving this limitation. The proposed assay method, relying on digital image processing, will be a quantitative assay for oncogen expression in individual cells which can be performed easily and quickly on the malignant cells of a specimen sample.