Preparation of algal cell wall-decomposing enzymes

藻类细胞壁分解酶的制备

• 批准号: 62470130
• 负责人: KITAMIKADO Manabu
• 金额: $ 2.18万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62470130/
• 关键词: Alga; Protoplast; Enzyme; beta-1, 4-Mannan; beta-1, 3-Xylan; Porphyran; Alginic acid; Cell wall; 海洋細菌; フコイダン; サザエ; 中腸腺; βー1,3ーキシラン; βー1,4ーマンナナーゼ; βー1,3ーマンナナーゼ; ポルフィラナーゼ; スサビノリ; アサクサノリ

Preparation of algal cell wall-decomposing enzymes.A search was undertaken to isolate bacteria from natural habitats which were able to degrade constitutive polysaccharides of algal cell wall ( beta-1,4-mannan, beta-1,3-xylan, alginate, porphyran, agar, and fucoidan). A lot of such bacteria were obtained, and the strains with a high productivity of polysaccharide-degrading enzyme were selected. A bacterium with high productivity of beta-1,4-mannan-degrading enzyme was isolated from intestinal contents of a freshwater fish, and was assigned to tile genus Aeromonas. All of the bacteria selected for producers of the other polysaccharides-degrading enzymes were originated from marine environment, and were assigned to the genus Vibrio. On the other hand, activities of polysaccharide-degrading enzymes were determined for the viscera of many marine animals. The extract of hepatopancreas from the marine snails, topshell and abalone, possessed high activity of many polysaccharide-degrading enzymes. A mixture of bacterial beta-1,4-mannan-, beta-1,3-xylan-, and porphyran-degrading enzymes released proto plast from thalli of a red alga, Porphyra yezoensis. The mixture of several bacterial enzymes and the extract of topshell hepatopancreas was a useful tool to release protoplast from many species of green, brown, and red algae.

藻类细胞壁分解酶的制备。研究人员从自然生境中分离出能够降解藻类细胞壁组成多糖的细菌（β -1,4-甘露聚糖、β -1,3-木聚糖、海藻酸盐、卟啉、琼脂和岩藻聚糖）。获得了大量的多糖降解菌，筛选出了多糖降解酶产率高的菌株。从淡水鱼肠道内容物中分离到一株高产β -1,4-甘露聚糖降解酶的细菌，并将其归入气单胞菌属。其他多糖降解酶的产生菌均来源于海洋环境，并归属于弧菌属。另一方面，测定了许多海洋动物内脏中多糖降解酶的活性。海螺、顶壳和鲍鱼肝胰腺提取物对多种多糖降解酶具有较高的活性。细菌-1,4-甘露聚糖、-1,3-木聚糖和卟啉降解酶的混合物从一种红藻紫菜的菌体中释放出原质体。几种细菌酶与顶壳肝胰脏提取物的混合物是释放多种绿、褐、红藻原生质体的有效工具。

项目成果

T.Aoki: "Purification and Characterization of an Endo-β-1,3-Xylanase from Vibrio sp." Nippon Suisan Gakkaishi. 54. 277-281 (1988)

T. Aoki：“弧菌内切-β-1,3-木聚糖酶的纯化和表征”，Nippon Suisan Gakkaishi，54. 277-281 (1988)。

T. Aoki, T. Araki, and M. Kitamikado: "Purification and Characterization of an Endo-beta-1, 3-Xylanase form Vibrio sp." Nippon Suisan Gakkaishi 54, 277-281, 1988.

T. Aoki、T. Araki 和 M. Kitamikado：“弧菌属 Endo-beta-1, 3-木聚糖酶的纯化和表征”。

荒木利芳: 日本水産学会誌. 54. (1988)

荒木俊义：日本水产学会杂志 54。（1988）

北御門学: "アルギン酸分解酵素産生細菌の自然界からの分離" 日本水産学会誌. 55. 709-713 (1989)

Manabu Kitamikado：“从自然界中分离产生藻酸盐降解酶的细菌”日本水产学会杂志 55. 709-713 (1989)。

K. Yamaguchi, T. Araki, T. Aoki, C.-H. Tseng, and M. Kitamikado: "Algal Cell Wall-Degrading Enzymes from Viscera of Marine Animals." Nippon Suisan Gakkaishi 55, 105-110, 1989.

K. Yamaguchi、T. Araki、T. Aoki、C.-H。