Structure and Function of High Molecular Weight Cytochrome C in Desulfovibrio

脱硫弧菌高分子量细胞色素C的结构和功能

基本信息

  • 批准号:
    62470144
  • 负责人:
  • 金额:
    $ 3.58万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1987
  • 资助国家:
    日本
  • 起止时间:
    1987 至 1988
  • 项目状态:
    已结题

项目摘要

1. High molecular weight cytochrome c from desulfovibrio vulgaris hildenborough has been isolated, purified and crystallized. The molecular weight was estimated to be 75,000 as the mean value from the results of gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The protein contains 16 c-type heme groups per molecule. The purified high molecular weight cytochrome c shows typical c-type cytochrome absorption spectra, with an -peak at 553.2 nm in the ferroform. The amino acid composition shows that the protein contains a sufficient number of cysteine and histidine residue to account for the high content of heme groups. The amino acid composition of D. vulgaris Hildenborough high molecular weight cytochrome c has no similarities to the D. desulfuricans hexahemoprotein nitrate reductase. The high content of the heme groups might suggest that the cytochrome has an alternate intrinsjc biological function. Crystals of the high molecular weight cytochrome c have been … More grown from solutions of poly (ethylene glycol) 1000 or 2-methyl-2,4-pentanediol. The crystals are in space group P6_2 pr P6_4 with unit cell dimensions a=b=227.8, c=105.7 A and =120 . A large unit cell volume of 4.75・10^6 A^3 suggests that there are four or five protein molecules per asymmetric unit. They diffract to better than 4 A resolution and appear to be resistent to radiation damage. 2. The hydrogenase solubilized from the particulate fraction from Desulfovibrio vulgaris Miyazaki F has been crystallized. The apparently homogeneous hydrogenase has been separated into three components of similar molecular weight by high performance liquid chromatography on DEAE-Toyopearl. Each hydrogenase components was successfully crystallized by means of the vapour diffusion method with polyethylene glycol or 2-methyl-2,4-pentanediol as a precipitating agent. The crystal data: space group P212121, unit cell dimensions, a=102.1, b=126.8, c=66.9 A. Multiwavelenght anomalous dispersion method was applied to a structure analysis of hydrogenase and resulted to give plasuible irosulfur clusters. 3. Structure analysis of cytochrome c553 has been succeeded. Less
1.从普通海氏弧菌中分离、纯化并结晶了高分子量细胞色素c。根据凝胶过滤和十二烷基硫酸钠聚丙烯酰胺凝胶电泳的结果,分子量估计为75,000的平均值。该蛋白质每个分子含有16个C型血红素基团。纯化的高分子量细胞色素c显示出典型的C型细胞色素吸收光谱,铁仿在553.2 nm处有一个吸收峰。氨基酸组成表明,该蛋白质含有足够数量的半胱氨酸和组氨酸残基,以解释高含量的血红素基团。D. vulgaris Hildenborough高分子量细胞色素c与D.脱硫六血红素蛋白硝酸还原酶。高含量的血红素基团可能表明细胞色素具有交替的内在生物学功能。高分子量细胞色素c的晶体已经被 ...更多信息 从聚(乙二醇)1000或2-甲基-2,4-戊二醇的溶液中生长。晶体空间群为P6_2或P6_4,晶胞尺寸为a=B=227.8,c=105.7 A和=120。4.75·10^6埃^3的大晶胞体积表明,每个不对称单位中有4或5个蛋白质分子。它们的分辨率优于4 A,并且似乎对辐射损伤具有抵抗力。2.普通脱硫弧菌宫崎F的颗粒级分中溶解的氢化酶已结晶。用DEAE-Toyoprotein高效液相色谱法将表面均一的氢化酶分离成三个分子量相近的组分。用聚乙二醇或2-甲基-2,4-戊二醇作为沉淀剂,通过气相扩散法成功地结晶了每个氢化酶组分。晶体数据:空间群P212121,晶胞参数:a=102.1,B=126.8,c=66.9。用多波长反常色散法对氢化酶进行了结构分析,得到了一个易熔化的硫铁簇。3.细胞色素c553的结构分析已经成功。少

项目成果

期刊论文数量(28)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Y.Higuchi/Y.Morimoto/A.Horinaka/N.Yasuoka: J.Biochem. 103. 905-906 (1988)
Y.Higuchi/Y.Morimoto/A.Horinaka/N.Yasuoka:J.Biochem。
  • DOI:
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    0
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Y.Morimoto;Y.Higuchi;K.Wakamatsu;K.Oshima;K.Utimoto;N.Yasuoka: Bull.Chem.Soc.Jpn.Vol.62 No.2. 639-641 (1989)
Y.Morimoto;Y.Higuchi;K.Wakamatsu;K.Oshima;K.Utimoto;N.Yasuoka:Bull.Chem.Soc.Jpn.Vol.62 No.2。
  • DOI:
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    0
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  • 通讯作者:
Y.Higuchi;N.Yasuoka;M.Kakudo;Y.Katsube: Journal of Bilogical Chemistry. 262. 2823-2825 (1987)
Y.Higuchi;N.Yasuoka;M.Kakudo;Y.Katsube:生物化学杂志。
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    0
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YASUOKA Noritake其他文献

YASUOKA Noritake的其他文献

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{{ truncateString('YASUOKA Noritake', 18)}}的其他基金

Structure Study of Vitamin B12 enzyme, Diol Dehydratase
维生素B12酶、二醇脱水酶的结构研究
  • 批准号:
    09480180
  • 财政年份:
    1997
  • 资助金额:
    $ 3.58万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A Crystallographic Workbench and Its Use in Structure Analysis of Hydrogenase
晶体学工作台及其在氢化酶结构分析中的应用
  • 批准号:
    04403026
  • 财政年份:
    1992
  • 资助金额:
    $ 3.58万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
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