Structure and Function of High Molecular Weight Cytochrome C in Desulfovibrio

1.高分子重量细胞色素C来自Desulfovibrio Vulgaris Hildenborough已经被隔离、纯化和水晶化。据估计，分子重量为75，000，其平均值从凝胶过滤和十二烷基硫代聚酰胺凝胶电泳的结果中得出。蛋白质含有16 c型Heme组每分子。纯净的高分子重量细胞色素C显示典型的C型细胞色素吸收剂，在ferroform中有一个峰值在553.2纳米。氨基酸组成显示，蛋白质含有足够数量的半胱氨酸和历史居住者，以记录高含量的血红素组。D.的氨基酸成分。Vulgaris Hildenborough高分子重量细胞色素C与D没有相似之处。desulfuricans hexahemoprotein nitrate reductase。Heme组的高含量可能会认为细胞色素具有一个替代性的内部生物功能。高分子重量的晶体，细胞色素已经破裂。 ... More 从聚乙烯醇（ethylene glycol） 1000或2-甲基-2，4-戊二醇的溶液中生长。crystals are in space group P6_2 pr P6_4 with unit cell dimensions a=b= 227. 8， c= 105. 7 A and =120。A large unit cell volume of 4.75·10^6 A^3 suggests that there are four or five protein molecules per asymmetric unit.它们的分辨率比4个更好，而且似乎对辐射损伤有抵抗力。2.从Desulfovibrio vulgaris Miyazaki F的粒子部分中溶解的氢化酶已经被结晶化。“明显的同源氢化酶已被分离成类似分子重量的三个组分，由DEAE-Toyopearl上的高性能液体色谱法。”每个氢化酶组分都成功地结晶化，其意思是用聚乙烯醇或2-甲基-2，4-戊二醇作为预氮剂。晶体数据：空间组P212121，单元格尺寸， a=102.1， b=126.8， c=66.9 A。多波段异常分散方法已应用于氢化酶的结构分析，并最终用于提供可降解的异氮素簇。3.细胞色素C553的结构分析已经成功。Less（低）

1. High molecular weight cytochrome c from desulfovibrio vulgaris hildenborough has been isolated, purified and crystallized. The molecular weight was estimated to be 75,000 as the mean value from the results of gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The protein contains 16 c-type heme groups per molecule. The purified high molecular weight cytochrome c shows typical c-type cytochrome absorption spectra, with an -peak at 553.2 nm in the ferroform. The amino acid composition shows that the protein contains a sufficient number of cysteine and histidine residue to account for the high content of heme groups. The amino acid composition of D. vulgaris Hildenborough high molecular weight cytochrome c has no similarities to the D. desulfuricans hexahemoprotein nitrate reductase. The high content of the heme groups might suggest that the cytochrome has an alternate intrinsjc biological function. Crystals of the high molecular weight cytochrome c have been … More grown from solutions of poly (ethylene glycol) 1000 or 2-methyl-2,4-pentanediol. The crystals are in space group P6_2 pr P6_4 with unit cell dimensions a=b=227.8, c=105.7 A and =120 . A large unit cell volume of 4.75・10^6 A^3 suggests that there are four or five protein molecules per asymmetric unit. They diffract to better than 4 A resolution and appear to be resistent to radiation damage. 2. The hydrogenase solubilized from the particulate fraction from Desulfovibrio vulgaris Miyazaki F has been crystallized. The apparently homogeneous hydrogenase has been separated into three components of similar molecular weight by high performance liquid chromatography on DEAE-Toyopearl. Each hydrogenase components was successfully crystallized by means of the vapour diffusion method with polyethylene glycol or 2-methyl-2,4-pentanediol as a precipitating agent. The crystal data: space group P212121, unit cell dimensions, a=102.1, b=126.8, c=66.9 A. Multiwavelenght anomalous dispersion method was applied to a structure analysis of hydrogenase and resulted to give plasuible irosulfur clusters. 3. Structure analysis of cytochrome c553 has been succeeded. Less