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Study of the Polymer Compounds on the Stability of Enzyme Activity and its Builder Actions

高分子化合物对酶活性稳定性及其助剂作用的研究

基本信息

批准号：
62470145
负责人：
SATO Masako
金额：
$ 1.98万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

From the view points of energy-saving, environmental protection and high performance of enzyme-containing detergents, the effects of the construction of the builders on the stability of enzyme activity and its builder actions were studied. The activities of enzymes, protease or lipase, were lowered by anionic surfactant, dbs, and some electrolytes in proportion to the property of Ca^<2+> binding capactiy. The other, the protesase was activated by addition of nonelectrolyte PVAs and Ca^<2+>in spite of in the solution containing with 0.02% DBS and/or 0.02% electrolyte builder.It was resulted that the removal of soils should be much influenced by the activity of protease in the solution not only the soil of gelatin but also the particulate ferric oxide from cotton fabric soiled with gelatin and ferric oxide.The activity of lipase resulted in low stability in the alkaline solution,but the hydrolysates of triolein soil were certainly found on the washed fabric. These results suppose that the copolymers constructed ionicmonomer/nonionicmonomer should be preferred with the criterion rather the stabilization property of enzyme than the chetate property.Is was confirmed that the 2-hydroxyethylmethacrylate ; sodium styrene sulfonate ; sodium methacrylate copolymer is a excellent builder having the properities of low interaction with the enzymes and having the builder actions, such as Ca^<2+>binding capacity electrokinetic effect by the adsorption and suspension stability.

从节能、环保和高性能含酶洗涤剂的角度出发，研究了助洗剂的结构对酶活性稳定性的影响及其助洗剂作用。阴离子表面活性剂dbs和某些电解质可使酶（蛋白酶或脂肪酶）的活性降低，其降低程度与Ca^2+结合能力成正比。另一个，在含有0.02%DBS和/或0.02%Ca ^2+的溶液中，加入非电解质PVA和Ca^2+可激活蛋白酶结果表明，蛋白酶的活性不仅对明胶污垢的去除有很大影响，而且对染有明胶和氧化铁的棉织物中的氧化铁微粒的去除也有很大影响，蛋白酶的活性对明胶和氧化铁的去除有很大影响，而且对明胶和氧化铁的去除也有很大影响。脂肪酶的活性导致在碱性溶液中的稳定性低，但在洗涤织物上确实发现了三油酸甘油酯污垢的水解产物。这些结果表明，以酶的稳定性而不是螯合物的性质为标准，离子单体/非离子单体构成的共聚物应是优选的。甲基丙烯酸钠共聚物是一种优良的助洗剂，具有与酶的低相互作用的性质并具有助洗剂作用，如Ca^2+结合容量、电动效应、吸附作用和悬浮稳定性等。