Development of gene diagnosis and recombinant vaccine for animal morbilliviurs diseases

建立了牛瘟病毒(RV)m RNA文库，并测定了两个糖蛋白基因(H、F蛋白)的核苷酸序列。1)由其核苷酸序列推导出的H蛋白具有独特的结构，其C末端突出于被膜外。用10种不同类型的抗合成多肽抗体进行的表位分析证实了预测的结构。2)推测的F蛋白在裂解的F2片段上有一个蛋白水解酶切位点和糖基化位点。3)含有RV-H基因的痘苗病毒重组DNA疫苗在兔模型系统中表现出很强的免疫效果，临床、病毒学和病理学研究证实重组疫苗对免疫动物具有完全保护作用。研究了犬瘟热病毒(CDV)的神经毒力。4)神经细胞适应的CDV显示出较高的神经毒力，并因适应而改变了其基因组和糖蛋白。5)对一组日本猴进行了CDV自然感染和病毒性脑炎的研究。这是世界上第一个关于灵长类动物对CDV脑炎具有自然易感性的报道。6)松鼠猴属于新大陆猴，被证实对CDV脑炎具有高度敏感性，并在急性脑炎期间表现出癫痫发作。它将成为治疗病毒性癫痫的极佳模型。

The cDNA library of rinderpest virus (RV) mRNA has been prepared and nucleotide sequence of both glycoprotein genes (H, F protein) has been completely determined.1) Deduced H protein from its nucleotide sequence has a unique structure in which C-terminus projects out of envelope. This predicted structure was confirmed by epitope analysis using ten different types of anti-syntheitc peptide antibodies.2) Deduced F protein has a site of proteolytic cleavage and glycosylation sites on the cleft F2 fragment. This was confirmed by isotope labeling of RV-infected cells.3) Recombinant DNA vaccine consisted of vaccinia virus having RV-H gene showed very strong efficacy in a rabbit model system and complete protection of immunized animals with the recombinant vaccine has been confirmed by clinical, virological and pathological investigations.Neurovirulence of canine distemper virus (CDV) was studied.4) Neural cell-adapted CDV showed relatively high neurovirulence and changed its genome and glycoproteins as a result of adaptation.5) Natural infection and viral encephalitis by CDV in a group of Japanese monkeys were studied. This is the first report in the world that primate has a natural susceptibility to CDV- encephalitis.6) Squirrel monkey which belongs to new world monkeys, was confirmed to have high susceptibility to CDV-encephalitis and to show epilectic seizure during acute encephalitis. It will become an excellent model for viral epilepsy.