Development of gene diagnosis and recombinant vaccine for animal morbilliviurs diseases

本文制备了牛瘟病毒（RV）mRNA的cDNA文库，并测定了两个糖蛋白基因（H、F蛋白）的核苷酸序列。10种不同类型的抗合成肽抗体的表位分析证实了这一预测结构。2）推导的F蛋白在裂F2片段上具有蛋白水解切割位点和糖基化位点。3）由具有RV-H基因的牛痘病毒组成的重组DNA疫苗在兔模型系统中显示出非常强的效力，并且用该重组疫苗免疫的动物的完全保护已经被临床证实，对犬瘟热病毒（CDV）的神经毒力进行了研究。适应后的CDV表现出较高的神经毒力，并改变了基因组和糖蛋白。5）研究了CDV在一组日本猴中的自然感染和病毒性脑炎。这是世界上首次报道灵长类动物对CDV脑炎具有自然易感性。6）松鼠猴是新大陆猴的一种，被证实对CDV脑炎具有高度易感性，并在急性脑炎时表现出癫痫发作。它将成为一个很好的病毒性癫痫模型。

The cDNA library of rinderpest virus (RV) mRNA has been prepared and nucleotide sequence of both glycoprotein genes (H, F protein) has been completely determined.1) Deduced H protein from its nucleotide sequence has a unique structure in which C-terminus projects out of envelope. This predicted structure was confirmed by epitope analysis using ten different types of anti-syntheitc peptide antibodies.2) Deduced F protein has a site of proteolytic cleavage and glycosylation sites on the cleft F2 fragment. This was confirmed by isotope labeling of RV-infected cells.3) Recombinant DNA vaccine consisted of vaccinia virus having RV-H gene showed very strong efficacy in a rabbit model system and complete protection of immunized animals with the recombinant vaccine has been confirmed by clinical, virological and pathological investigations.Neurovirulence of canine distemper virus (CDV) was studied.4) Neural cell-adapted CDV showed relatively high neurovirulence and changed its genome and glycoproteins as a result of adaptation.5) Natural infection and viral encephalitis by CDV in a group of Japanese monkeys were studied. This is the first report in the world that primate has a natural susceptibility to CDV- encephalitis.6) Squirrel monkey which belongs to new world monkeys, was confirmed to have high susceptibility to CDV-encephalitis and to show epilectic seizure during acute encephalitis. It will become an excellent model for viral epilepsy.