HUMAN PARAINFLUENZA TYPE 2 VIRUS GLYCOPROTEIN GENES

本研究拟构建人副流感病毒2型（PI 2）cDNA文库， 将病毒mRNA导入质粒载体中。 文库将按菌落筛选 杂交方法 编码包膜糖蛋白的基因将被 在Southern印迹分析中使用特异性探针鉴定， mRNA和序列分析。 一旦获得全长克隆， 将评价克隆的基因以确定它们的免疫原性。 长 术语目标是利用这些糖蛋白作为 控制副流感病毒感染的多价亚单位疫苗。

We propose to construct a cDNA library of human parainfluenza Type 2 (PI2) virus mRNAs into a plasmid vector. The library will be screened by colony hybridization. The genes encoding envelope glycoproteins will be identified using specific probes in Southern blot analysis, translation of mRNAs and by sequence analysis. Once full length clones are obtained, the cloned genes will be evaluated to determine their immunogenicity. The long term objective is to utilize these glycoproteins as components of a multivalent subunit vaccine to control parainfluenza virus infection.