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Studies on the organ-associated natural killer cells (pit cells)

器官相关自然杀伤细胞（凹坑细胞）的研究

基本信息

批准号：
62480091
负责人：
YAMAUCHI Shoji
金额：
$ 3.58万
依托单位：
Department of Anatomy, Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480091/
关键词：
natural killer cells rod-cored vesicles tumor cell lysis liver 腫瘍細胞傷害微小管ピット細胞 NK細胞 BRM OKー432 糖脂質

项目摘要

This study has revealed electron microscopically that liver-associated natural killer (NK) cells are the important constituent of the defense system of the liver against the tumor growth or metastasis.1. Ultrastructural study of in vivo cytolysis of tumor cells by liver-associated NK cells. (i) Intraportally injected Yac-1 tumor cells were cytolysed by NK cells inside the sinusoids. It was suggested that granules and rod-cored vesicles of NK cells would be important in the cytolysis. NK cells could also phagocytize Yac 1 cells in cooperation with associating Kupffer cells. This study is the first to show the direct evidence of in vivo NK cytolysis. (ii) In the human primary hepatoma, we observed that NK cells infiltrated into the tum or but they did not approach to the tumor cells due to the fibrous capsule.2. Distribution of cell organelles of NK cells during the in vitro cytolysis. (i) When NK cells which were isolated from the spleen or liver were co-cultured with Yac-1 cells, they contacted with and extended the projections toward the tumor cells. The cell organelles of NK cells were reoriented toward the Yac-1 cells, and the rod-cored vesicles were aligned along the microtubules which ran to the contact area from the centrioles. This suggests that rod-cored vesicles, highly characteristic to NK cells, are important in NK cytolysis. (ii) We are now developing the cinematographic analysis about the process of NK cytolysis.

本研究通过电镜观察发现，肝相关自然杀伤（NK）细胞是肝脏抵抗肿瘤生长或转移防御系统的重要组成部分.肝相关NK细胞体内杀伤肿瘤细胞的超微结构研究。(i)门静脉内注射的Yac-1肿瘤细胞被窦状隙内的NK细胞溶解。提示NK细胞的颗粒和杆状核囊泡在细胞溶解中起重要作用。NK细胞也能协同相关枯否细胞吞噬Yac 1细胞。这项研究是第一个显示体内NK细胞溶解的直接证据。(ii)在人原发性肝癌中，我们观察到NK细胞向肿瘤浸润，但由于纤维包膜的存在，NK细胞不能接近肿瘤细胞. NK细胞体外溶胞过程中细胞器的分布。(i)当从脾或肝分离的NK细胞与Yac-1细胞共培养时，它们与肿瘤细胞接触并向肿瘤细胞延伸突起。NK细胞的细胞器被重新定向到Yac-1细胞，并且杆状核囊泡沿着微管排列，微管从中心粒延伸到接触区域。这表明，杆状核囊泡，NK细胞的高度特征，是重要的NK细胞溶解。(ii)我们正在发展NK细胞溶解过程的电影分析。