Functional Morphology of the Feto-Placental Unit in Domestic Animals.

1.通过注射树脂对胎盘血管构筑进行了大体和扫描电子显微镜观察。结果：1.阐明了母牛胎盘、母羊胎盘、母体胎盘和母体胎盘的一般血管构筑、妊娠期的时间变化以及因胎盘类型而引起的物种差异。除了对猪胚胎脑垂体的组织发生进行了光镜和电子显微镜的研究外，还用ACTH、GH、LH和PRL抗体进行了酶免疫学研究。明确了垂体前叶各腺体细胞的出现阶段。此外，还阐明了胎儿期各腺体细胞的分布特点。通过组织计量学和羟类固醇脱氢酶(HSD)活性的研究，明确了胎猪肾上腺的区带和内分泌活性。在胎儿早期，肾上腺主要由胎儿皮质细胞组成。在妊娠的后半期，明确的皮质，特别是束状带逐渐发育。与此同时，胎儿皮质逐渐退缩。胎儿皮质和束状带的HSD活性相似，因此与胎儿胎盘单位有关的重要作用被怀疑。对胎猫肾上腺的组织发生进行了组织计量学、HSD活性检测和电子显微镜观察。在猫的案例中，即使在怀孕后期，胎儿皮质的细胞也没有表现出退化的迹象。相反，胎儿皮质似乎并入了网状带.因此，胎儿皮质似乎在妊娠中期起作用，束状带在此之后成功。

1. Macroscopical and scanning electron microscopical investigations on a placental vasular architecture by means of the resin injection were undertaken. General vascular architectures, chronological changes during pregnancy and species differences due to the placental types were clarified in fetal placenta of the cow, maternal and fetal placentae of the bitch and maternal placenta of the suncus.2. In addition to the microscopical and electron microscopical study on the histogenesis of the porcine fetal hypophysis, an enzyme immunological investigation by means of antibodies for ACTH, GH, LH, and PRL was also undertaken. Stages of an appearance of each gland cell in the anterior pituitary gland were clarified. Furthermore, the characteristics of the distribution pattern of each gland cell during the fetal life were also clarified.3. Zonation and the endocrine activity of the fetal porcine adrenal gland were clarified by means of the histometry as well as the investigation on activities for hydroxysteroid dehydrogenase (HSD). In an early fetal stage, the adrenal gland was mainly consisted from cells of the fetal cortex. In a latter half of pregnancy, a definitive cortex particularly the zona fasciculate developed progressively. Meanwhile, the fetal cortex involuted gradually. Both the fetal cortex and the zona fasciculata showed the similar activities for HSD, hence the important role relating to a feto-placental unit was suspected.4. Histometry, a detection for HSD activities and an electron microscopical investigations were undertaken on a histogenesis of the fetal cat adrenal gland. In a case of the cat, cells of the fetal cortex did not show a sign of involution even in a late stage of pregnancy. Instead, the fetal cortex as seemed to be incorporated into the zona reticularis. Accordingly, the fetal cortex seemed to play a role up to the middle of pregnancy and the zona fasciculata succeeds thereafter.