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Polymorphonuclear leukocytes as an inflammatory hormone-producing argan.

多形核白细胞作为产生炎症激素的摩洛哥坚果。

基本信息

批准号：
62480143
负责人：
YOSHINAGA Masaru
金额：
$ 4.42万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Polymorphonuclear leukocytes (PMN) have been believed to serve as scavenger cells for foreign substances. Just recently, we have realized that PMN may have an important role as an inflammatory hormone-producing cells in addition to the well known role as scavengers, because we have noted that PMN were producing an immune potentiation factor during the early stage of inflammation in peritoneal cavity of rabbits. This immune potentiation factor was considered to be newly synthesized by PMN after their infiltration into the inflammatory site. The basis of the assumption was (a) Blood PMN do not have such factor. (b) Inflammatory PMN quickly changed to have the factor at the inflammatory site. (c) Blood PMN could be triggered to produce the factor in vitro by stimulation of a combination with endotoxin and shaking culture. This in vitro production was completely inhibited by several inhibitors of protein synthesis. (d) 14C-labelled amino acids were incorporated into the nowly syntheized im … More mune potentiation factor.We have isolated the inflammatory immune potentiation factor and determined its partial structure. Furthermore, we have succeeded to choose a cDNA clone coding fot the immune potentiation factor and determined its structure. The structure closely resembled that of human and murine IL 1 then, we concluded that this inflammatory factor is IL 1 of rabbit.Then, we were faced the problem of consideration whether this IL 1 is really produced by PMN, because macrophages were generally believed to be the major producer of IL 1 but we finally proved the production of IL 1 by PMN as follows: (a) A highly purified PMN from the early inflammatory peritoneal exudate cells contained IL 1. (b) Also, the purified PMN express the mRNA for IL 1 .(c) It was histochemically proved that about 99% of IL 1 -containing cells at inflammatory site were PMN. (d) Only a few percentage of IL 1 -containing cells were macrophages during the entire course of the inflammation. The IL 1 is now considered to be one of important inflammatory hormone and we considered that PMN also may produce important substances other than IL 1 at the inflammatory site. We investigated this possibility and found that PMN kept their protein synthesis capability during a long period of inflammation until 48 hrs. The inflammatory PMN produced at least 8 kinds of secreting proteins after arrival into the invlammatory site. The biolobical functions of these newly syntheseized substances are the matter of further investigation. Less

多形核白细胞（PMN）被认为是外来物质的清道夫细胞。最近，我们发现PMN在兔腹腔炎症早期产生免疫增强因子，从而认识到PMN除了作为清道夫的作用外，还可能作为产生炎性细胞的重要作用。这种免疫增强因子被认为是由PMN浸润到炎症部位后新合成的。假设的基础是（a）血液中性粒细胞不具有此类因素。(b)炎性中性粒细胞迅速改变，在炎症部位有因子。(c)血中性粒细胞在体外经内毒素刺激和振荡培养后可产生该因子。这种体外生产完全被几种蛋白质合成抑制剂所抑制。(d)14 C标记的氨基酸被掺入到现在合成的肌肉中， ...更多信息免疫增强因子，我们分离了炎性免疫增强因子，并测定了其部分结构。此外，我们还成功地筛选到了一个编码免疫增强因子的cDNA克隆，并确定了其结构。由于巨噬细胞是IL-1的主要产生者，因此，我们面临的问题是：这种IL-1是否真的是由PMN产生的？（a）从早期炎性腹膜渗出液细胞中高度纯化的PMN含有IL 1。(b)此外，纯化的PMN表达IL 1的mRNA。(c)组织化学证实炎症部位含IL-1细胞约99%为中性粒细胞。(d)在整个炎症过程中，只有少数含IL 1的细胞是巨噬细胞。目前认为IL-1是一种重要的炎性激素，我们认为PMN在炎症部位也可能产生IL-1以外的重要物质。我们研究了这种可能性，发现PMN在长时间的炎症过程中保持其蛋白质合成能力，直到48小时。炎症中性粒细胞到达炎症部位后至少产生8种分泌蛋白。这些新合成的物质的生物学功能有待进一步研究。少