Synergistic effect of the cell-kinetic oriented chemo-endocrine therapy on mammary cancer.

分别采用MCF7和SC115两种类型的乳腺癌进行体外和体内实验。体外培养的MCF7是雌激素依赖性人乳腺癌细胞系。DDS雄性小鼠维持BC115为雄激素依赖性乳腺癌。体外给予他莫昔芬，体内睾丸切除术作为内分泌治疗。5FU体外给药1 g/ml，体内给药40mg/kg。流式细胞术分析各肿瘤细胞动力学。他莫昔芬和睾丸切除术均能抑制肿瘤生长。这些内分泌治疗对肿瘤细胞动力学的影响被认为是g_1阻滞。5FU对肿瘤细胞动力学的影响是在暂时的S期抑制中得到的。通过对比三组分别在睾丸切除术前、术后1周、2周给药5FU对DDS小鼠BC115生长的协同抑制作用，观察化学内分泌治疗对BC115生长的协同抑制作用。预先给药5FU对肿瘤生长有协同抑制作用。这种抑制作用是由S相组分的长期抑制引起的。

Two types of breast cancer MCF7 and SC115 were used in vitro and in vivo experment respectively. MCF7 culturing in vitro was estrogen dependent human breast cancer cell line. BC115 maintaining on DDS male mice was androgen dependent breast cancer. Tamoxifen was administered in vitro and testectomy was carriedtout in vivo as the endocrine therapy. 5FU was used 1 g/ml in vitro and 40mg/kg in vivo as the chemotherapy. Cell kinetics of each tumor were analysed with flow cytometry.Both of tamoxifen and testectomy reduced tumor growth. These effects of the endocrine therapy upon the tumor cell kinetics were thought to be g_1 block. The effect of 5FU upon the tumor cell kinetics was obtained in temporary S phase depression.Synergistic inhibitory effect of the chemoendocrine therapy on BC115 growth in DDS mice were observed in comparing with three groups which divided by 5FU administration at the different times before, 1 week, 2 weeks after testectomy performed. Anteceding administration of 5FU showed the synergistic inbitory effect on tumor growth. This inbitory effect resulted cytometrically from the long term depression of S phase fraction.