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Synergistic effect of the cell-kinetic oriented chemo-endocrine therapy on mammary cancer.

细胞动力学导向的化学内分泌疗法对乳腺癌的协同作用。

基本信息

批准号：
62480273
负责人：
ABE Rikiya
金额：
$ 1.79万
依托单位：
Fukushima Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480273/
关键词：
TMX Testectomy 5FU chemo-endocrine therapy Breast cancer FCM 細胞動態フローサイトメトリー TMX,除睾術 5FU MCF7 内分泌治療細胞四粒フローサイトメトメトリー TAM

项目摘要

Two types of breast cancer MCF7 and SC115 were used in vitro and in vivo experment respectively. MCF7 culturing in vitro was estrogen dependent human breast cancer cell line. BC115 maintaining on DDS male mice was androgen dependent breast cancer. Tamoxifen was administered in vitro and testectomy was carriedtout in vivo as the endocrine therapy. 5FU was used 1 g/ml in vitro and 40mg/kg in vivo as the chemotherapy. Cell kinetics of each tumor were analysed with flow cytometry.Both of tamoxifen and testectomy reduced tumor growth. These effects of the endocrine therapy upon the tumor cell kinetics were thought to be g_1 block. The effect of 5FU upon the tumor cell kinetics was obtained in temporary S phase depression.Synergistic inhibitory effect of the chemoendocrine therapy on BC115 growth in DDS mice were observed in comparing with three groups which divided by 5FU administration at the different times before, 1 week, 2 weeks after testectomy performed. Anteceding administration of 5FU showed the synergistic inbitory effect on tumor growth. This inbitory effect resulted cytometrically from the long term depression of S phase fraction.

本实验分别采用两种乳腺癌细胞株MCF 7和SC 115进行体外和体内实验。体外培养的MCF 7是雌激素依赖的人乳腺癌细胞系。在DDS雄性小鼠上维持的BC 115是雄激素依赖性乳腺癌。三苯氧胺在体外给药，替诺明在体内进行内分泌治疗。5 FU体外化疗剂量为1g/ml，体内化疗剂量为40 mg/kg。用流式细胞仪分析每个肿瘤的细胞动力学。他莫昔芬和替莫昔芬都减少了肿瘤的生长。内分泌治疗对肿瘤细胞动力学的影响被认为是g_1阻滞。以肿瘤细胞S期暂时性抑制为指标，观察5 FU对肿瘤细胞生长的影响，并与化疗前、化疗后1周、化疗后2周3组比较，观察化疗内分泌治疗对DDS小鼠BC 115肿瘤生长的协同抑制作用。5 FU预处理对肿瘤生长有协同抑制作用。这种抑制作用是由于S期细胞比例长期下降所致。