Experimental investigation of heart-lung transplantation

心肺移植的实验研究

• 批准号: 62480292
• 负责人: FURUSE Akira
• 金额: $ 4.29万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480292/
• 关键词: Heart-lung transplantation; Japanese monkey (Macaca Fuscata); Allotransplantation and Autotransplantation; Chest roentogenogram; Asynchronous rejection of the heart and the lung; Tracheal findings; Myosin light chain level; 気管所見; ミオシン軽鎖; 血漿中ミオシン軽

Experimental evaluations were done in Japanese monkeys (macaca fuscata) about orthotopic heart-lung allotransplantation, orthotopic heart-lung autotransplantation, and heterotopic heart allotransplantation.In orthotopic heart-lung allotransplantation,eight recipient monkeys survived for three to thirty three days. Their respiratory pattern changed considerably after the transplantation.The chest roentogenogram in the early postoperative period showed the central cloudiness and enhancement of the interstitial change, alid these findings reached their peak between the fifth and eighth postoperative day. Pathological study revealed no obvious round cell infiltration in the lung of this stage.Asynchronous rejections of the heart and the lungs following the allotransplantation were observed in two monkeys. Moderate cardiac rejection and severe pulmonary edema without apparent round cell infiltration were observed in one monkey, and vascular type mild pulmonary rejection without cardiac reje … More ction was recognized in the etlier monkey.Histologic assessment of the donor trachea revealed that there was no significant change in the mucosal epithelium except for the squamous metaplasia which was observed in two monkeys. However,submucosal infiltration of round cell was observed in every monkey. The tracheas of allograft with moderate to severe rejection showed higher degrees of submoucosal cell infiltration than those with minimal to mild rejection.In the orthotopic heart-lung autotrazisplantation, seven monkeys survived two to five days. Postoperative chest roentogenogram showed peribronchial and perivascular cuffing in the early postoperative period. Peribronchial and perivascular edema without or with minimal round cell infiltration was observed microscopically.Measurement of the plasma myosin light chain level was done in the monkeys which were subjected to heterotopic cardiac allotransplantation,and it revealed to be of value in the monitoring of cardiac allograft rejection. Less

在日本猴（macaca fuscata）上进行了原位心肺同种异体移植、原位心肺自体移植和异位异位心肺移植的实验评估。在原位心肺同种异体移植中，8只受体猴存活了3至33天。移植后呼吸模式发生了显着变化。术后早期胸部X线检查显示中央混浊，间质改变增强，并在术后第5天至第8天达到高峰。病理学检查显示该阶段肺内未见明显圆形细胞浸润。两只猴同种异体移植后心脏和肺脏均出现非同步排斥反应。一只猴出现中度心脏排斥反应和重度肺水肿，无明显圆形细胞浸润；另一只猴出现血管型轻度肺排斥反应，无心脏排斥反应。供体气管组织学评估显示，除两只猴出现鳞状化生外，粘膜上皮无明显变化。然而，每只猴子均观察到粘膜下圆形细胞浸润。中度至重度排斥反应的同种异体移植物的气管比轻度至轻度排斥反应的粘膜下细胞浸润程度更高。在原位心肺自体移植中，7只猴子存活了2至5天。术后胸部X线检查显示术后早期有支气管周围和血管周围的袖带。镜下观察支气管周围和血管周围水肿，无或有极少的圆形细胞浸润。对异位心脏移植猴进行血浆肌球蛋白轻链水平测定，对监测同种异体心脏移植排斥反应有一定价值。较少的

项目成果

川内基裕、古瀬彰、進藤剛殻、松永仁、マティソン恵、横井泰、中島淳、吉竹毅ほか: "日本猿による同種同所心肺移植の検討。" 日本胸部外科学会雑誌. 36. 207-213 (1988)

Motohiro Kawauchi、Akira Furuse、Goka Shindo、Hitoshi Matsunaga、Megumi Mattison、Yasushi Yokoi、Jun Nakajima、Takeshi Yoshitake 等：“日本猴同种异体原位心肺移植的研究”。 36.207-213（1988）

川内基裕 他: 移植. 24. 73-79 (1989)

Motohiro Kawauchi 等人：移植。 24. 73-79 (1989)

Motohiro KAWAUCHI, Akira FURUSE, Goki SHINDO, Hitoshi MATSUNAGA, Megumi MATHISON, Yasushi YOKOI, Jun NAKAJIMA, and Tsuyoshi YOSHITAKE: "Orthotopic heart and lung transplantation in Japanese monkey." J Jap Assoc Thorac Surg 36(2):207-213, 1988.

Motohiro KAWAUCHI、Akira FURUSE、Goki Shindo、Hitoshi MATSUNAGA、Megumi MATHISON、Yasushi YOKOI、Jun NAKAJIMA 和 Tsuyoshi YOSHITAKE：“日本猴的原位心肺移植。”

川内基裕 他: 日胸外会誌. 37. (1989)

Motohiro Kawauchi 等人：日本胸科学会杂志 37。（1989 年）

Motohiro KAWAUCHI, Akira FURUSE, Megumi MATHISON, Jun NAKAJIMA, Yasushi YOKOI, Teruaki OKA, and Tsuyoshi YOSHITAKE: "Asynchronous rejection of heart and lungs following cardiopulmonary transplantation in Japanese monkey." Jap J Transplant 24(1):73-79, 198

Motohiro KAWAUCHI、Akira FURUSE、Megumi MATHISON、Jun NAKAJIMA、Yasushi YOKOI、Teruaki OKA 和 Tsuyoshi YOSHITAKE：“日本猴心肺移植后心脏和肺部的异步排斥。”