Experimental investigation of heart-lung transplantation

在日本猕猴（macaca fuscata）身上进行了原位心肺移植、自体心肺移植和异位心脏移植的实验评价。在原位心肺异体移植中，8只接受移植的猴子存活了3到33天。他们的呼吸模式在移植后发生了很大的变化。术后早期胸片显示中心浑浊，间质改变增强，在术后第5天至第8天达到高峰。病理检查未见明显圆形细胞浸润。在两只猴子身上观察到同种异体移植后心脏和肺的非同步排斥反应。其中1只猴子出现中度心脏排斥反应和严重肺水肿，无明显圆形细胞浸润；另1只猴子出现血管型轻度肺排斥反应，无心脏排斥反应。供体气管的组织学检查显示，除了在两只猴子身上观察到的鳞状化生外，粘膜上皮无明显变化。然而，每只猴子的粘膜下均可见圆形细胞浸润。中度至重度排斥反应的同种异体气管粘膜下细胞浸润程度高于轻度至轻度排斥反应的同种异体气管。在原位心肺自体移植中，7只猴子存活了2 - 5天。术后早期胸部x线摄影显示支气管周围和血管周围弯曲。镜下观察支气管周围和血管周围水肿，无或有少量圆细胞浸润。本文对异体心脏移植猴血浆肌球蛋白轻链水平进行了测定，对监测异体心脏移植排斥反应具有重要意义。少

Experimental evaluations were done in Japanese monkeys (macaca fuscata) about orthotopic heart-lung allotransplantation, orthotopic heart-lung autotransplantation, and heterotopic heart allotransplantation.In orthotopic heart-lung allotransplantation,eight recipient monkeys survived for three to thirty three days. Their respiratory pattern changed considerably after the transplantation.The chest roentogenogram in the early postoperative period showed the central cloudiness and enhancement of the interstitial change, alid these findings reached their peak between the fifth and eighth postoperative day. Pathological study revealed no obvious round cell infiltration in the lung of this stage.Asynchronous rejections of the heart and the lungs following the allotransplantation were observed in two monkeys. Moderate cardiac rejection and severe pulmonary edema without apparent round cell infiltration were observed in one monkey, and vascular type mild pulmonary rejection without cardiac reje … More ction was recognized in the etlier monkey.Histologic assessment of the donor trachea revealed that there was no significant change in the mucosal epithelium except for the squamous metaplasia which was observed in two monkeys. However,submucosal infiltration of round cell was observed in every monkey. The tracheas of allograft with moderate to severe rejection showed higher degrees of submoucosal cell infiltration than those with minimal to mild rejection.In the orthotopic heart-lung autotrazisplantation, seven monkeys survived two to five days. Postoperative chest roentogenogram showed peribronchial and perivascular cuffing in the early postoperative period. Peribronchial and perivascular edema without or with minimal round cell infiltration was observed microscopically.Measurement of the plasma myosin light chain level was done in the monkeys which were subjected to heterotopic cardiac allotransplantation,and it revealed to be of value in the monitoring of cardiac allograft rejection. Less