Basic Research of Heart Lung Transplantation

心肺移植基础研究

• 批准号: 02454325
• 负责人: FURUSE Akira
• 金额: $ 3.71万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454325/
• 关键词: pulmonary transplantation; Japanese monkey; xenotransplantation; FK506; pleural lavage fluid; flow cytometry; MHC antigen; Immunofluoro staining; 同種肺移植; 病理組織所見; CD4; CD8; ニホンザル; フロ-サイトメトリ-; 拒絶反応; 左肺同種同所性移植; 急性拒絶反応; FKー506; 胸部X線所見; 肺野不透明

Immunological response to allo- and xeno-lung graft was studied in this project using single lung transplantation model with Japanese Macaque monkeys. (1) Monkeys with lung allograft without immunosuppression showed consolidation by chest roentgenogram within 7 days and microscopic examination revealed rejected allograft. Monkeys with FK506 immunosuppression survived from 5 to 182 days and 6 allograft out of seven were considered to be functioning at the time of animal death or sacrifice. FK506 was considered as effective immunosuppressant for allotransplantation of the lung. (2) The feasibility of pleural lavage fluid(PLF) for the diagnosis of pulmonary allo- and xenograft rejection was studied. The change of lymphocyte subsets in PLF was independent from that in peripheral blood, and that CD4+/CD8+ ratio of lymphocytes in PLF decreased as the grafted lung was rejected. (3) Allografted lungs were examined with immunofluorescent staining methods. The expression of the MHC class 1 and 2 antigens were recognized even under clinically sufficient immunosuppression. CD4(+) cells, CD8(+) cells, and CD20(+) cells in the allograft was considered to play important role in the allograft rejection.

本研究采用日本猕猴单肺移植模型，研究同种和异种肺移植的免疫反应。(1)未使用免疫抑制剂的同种异体肺移植猴在7天内胸部X线片显示实变，显微镜检查显示移植物排斥。接受FK 506免疫抑制的猴存活5 - 182天，认为7只同种异体移植物中有6只在动物死亡或处死时仍有功能。FK 506是一种有效的同种异体肺移植免疫抑制剂。(2)目的：探讨胸腔灌洗液（PLF）诊断同种和异种肺移植排斥反应的可行性。PLF中淋巴细胞亚群的变化与外周血无关，随着移植肺排斥反应的发生，PLF中淋巴细胞CD 4 +/CD 8+比值下降。(3)免疫荧光染色法观察移植肺组织。即使在临床充分的免疫抑制下，也能识别MHC 1类和2类抗原的表达。移植物中的CD 4（+）、CD 8（+）和CD 20（+）细胞在移植排斥反应中起重要作用。

项目成果

柳生 邦良、古瀬 彰、Borst,llG.: "心エコ-法による移植心の急性拒絶反応の診断" 日本胸部外科学会雑誌. 38(suppl). 287 (1990)

Kuniyoshi Yagyu，Akira Furuse，Borst，llG.：“通过超声心动图诊断移植心脏的急性排斥”，日本胸外科学会杂志 38（增刊）。

古瀬 彰、中島 淳: "ドナ-とレシピエントの連携(臓器移植ネットワ-クシステム)" 「心臓移植のための指針」 1990年度版 日本心臓移植研究会編. 56-60 (1990)

Akira Furuse、Jun Nakajima：“供者和受者之间的合作（器官移植网络系统）”《心脏移植指南》1990年版，日本心脏移植研究小组编辑56-60（1990）。

Kawauchi M.,Yazaki Y.,NakaJima J.,Furuse A.,et al.: "Diagnosis of cardiac allograft rejection by the detection of circulating plasma cardiac myosin light chains." Japanese Journal of Surgery. 20. 212-216 (1990)

Kawauchi M.、Yazaki Y.、NakaJima J.、Furuse A.等人：“通过检测循环血浆心肌肌球蛋白轻链来诊断心脏同种异体移植排斥反应。”

古瀬 彰: "心肺同時移植に関する基礎的研究 昭和62年度〜平成元年度 科学研究費補助金(一般研究B)研究成果報告書 No.62480292" 59 (1990)

Akira Furuse：“同时心肺移植的基础研究1985-1989科学研究补助金（一般研究B）研究结果报告第62480292号”59（1990）

Kawauchi M, Nakajima J, Furuse A: "Heart transplantation and the bridge use of the artificial heart" Gekasinryo. 32. 1282-1288 (1992)

Kawauchi M、Nakajima J、Furuse A：“心脏移植和人造心脏的桥梁使用”Gekasinryo。