Experimental Research of Xeno Heart Transplantation

1.应用心肺机对猕猴心脏进行血液灌流后的组织病理学变化：(1)例1：ABO血型不合人全血冠脉灌流(2)例2：ABO血型相合人全血冠脉灌流(3)例3：ABO血型相合和血浆去血型冠脉灌流病例1、2在冠脉灌流开始后15分钟内出现水肿和弥漫性出血。30分钟后，猕猴心脏出现室颤并停止跳动。例3跳动良好，观察过程中未见宏观变化。RV、LV、IVS组织病理学检查，病例1、2可见严重的间质出血、收缩带坏死、单核细胞浸润及动脉内血栓形成。但所有病例均可见斑片状坏死。有人认为，这些组织病理变化，如超急性排斥反应，是由于人类血浆中的异种抗体所致。A…用人类同种异体抗血清和抗人类白细胞抗原单抗进一步分析交叉反应性猕猴组织相容性抗原。对提交给第十届国际组织相容性研讨会的43份人类白细胞抗原I类和81份抗人类白细胞抗原I类单抗进行补体依赖细胞毒试验。在463份同种异体抗血清中，有136份因异种抗体反应强烈。跨越物种屏障的人类白细胞抗原定义为：人类白细胞抗原-A1、-A2、-B40(W60、W61)、-B5(51、W52)、-B27、-21、-CW1和CW2。定义的多特异性抗原有A10(25，26)-Aw19(31，32，w33)、B5-B35-B15(w62，w63)、部分B7-B27、部分B7-B40(w60，w61)和B12(44，45)-B21-B5。提示组织相容性抗原有可能跨越物种的屏障，用普通的抗血清来确定，并能从获得的匹配等级中选择合适的供体用于异种移植。较少

1. Histopathological Changes on Macaque Hearts Perfused with Human Blood by Using Cardio-pulumonary Machine.(1) Case 1 : Coronary Perfusion by Using ABO Imcompatible Human Whole Blood(2) Case 2 : Coronary Perfusion by Using ABO Compatible Human Whole Blood(3) Case 3 : Coronary Perfusion by Using ABO Compatible and Plasma Removed Human BloodAt the macroscopy, case 1 and 2 were observed edema and diffuse bleeding within 15 minutes after coronary perfusion started. After 30 minutes, Macaque hearts resulted in ventricular fibrillation and arrested. Case 3 was well beating and there was no macroscopic changes during observation. At the histopathological examination of RV, LV and IVS, case 1 and 2 were observed severe interstitial bleeding, contraction band necrosis, mononuclear infiltration and intra-arterial thrombus. But patchy necrosis was observed all cases. It was suggested that these histopathological changes like a hyperacute rejection were due to xeno-antibodies in human plasma.2. A … More nalysis of cross-reactive Macaque histocompatibility antigens by using HLA alloantisera derived from human and monoclonal antibodies against HLA antigens.Four hundred and sixty three HLA-class I and 81 monoclonal antibodies against HLA-class I antigens, submitted to 10th International Histocompatibility Workshop, were typed by Macaque lymphocytes using complement dependent cytotoxicity test. In 136 of 463 alloantisera were reacted strongly because of xeno-antibodies. HLA antigens, across the barrier of species, defined mono-specific were HLA-A1, -A2, -B40 (w60, w61), -B5 (51, w52), -B27, -21, -Cw1 and Cw2. HLA Antigens defined multispecific were A10 (25, 26) -Aw19 (31, 32, w33), B5-B35-B15 (w62, w63), a part of B7-B27, a part of B7-B40 (w60, w61) and B12 (44, 45) -B21-B5. It was suggested the possibility that histocompatibility antigens, across the barrier of species, were defined by using common HLA antisera and were able to select the suitable donor for xeno-transplantation from the obtained matching grades. Less