Basic research for mini-pig production to induction og human MHC genes

猪主要组织相容性复合体(SLA)编码了大量的基因，包括猪7号染色体上的I、II和III类基因。SLA的结构和功能特性证实了它与人类主要组织相容性复合体(HLA)的相似性。本研究克隆了改良哥廷根CSK小型猪和起源于鹿儿岛大学的CLAWN小型猪SLA-II类基因(DRB、DQB、DQA、DNA)的全长cDNA克隆，分析了SLA-II类基因与人类白细胞抗原II类基因的氨基酸序列的一致性，并从CSK和CLAWN小型猪的脾中提取了mRNA。构建基因文库，以人类白细胞抗原II类基因克隆为探针进行筛选。这些克隆编码250个氨基酸的初级翻译产物，其中包含27个氨基酸的信号肽，与人类白细胞抗原II类基因的氨基酸序列进行比较，氨基酸比较分析显示SLA II类基因与人类白细胞抗原-DRB、-DRA、-DQA和-DNA分子的氨基酸序列具有较高的同源性(分别为75%、87%、86%和83%)。SLA基因的分析对于建立一种小型猪来诱导即将到来的异种移植供体的人类白细胞抗原基因具有重要意义。

The swine major histocompatibility complex (SLA) is encoded a large number of genes including the class I, II, and III on swine chromosome 7. Structural and functional properties of the SLA confirm the similarity to that of human major histocompatibility complex (HLA). In this study, we have isolated a full-length cDNA clone of SLA class II (DRB, DQB, DQA, DNA) genes of CSK minipig (modified Gottingen) and CLAWN minipig (originated Kagoshima Univ.) , and analyzed the coincidence of amino acid sequence between SLA class II genes and HLA class II genes to induction of HLA genes to minipig.The mRNA was extracted from CSK and CLAWN minipig spleen. The cDNA library was constructed and screened by the HLA class II cDNA clone as a probes. These clone encoded a primary translated products of 250 amino acids, which includes a signal peptide of 27 amino acids were compared to amino acid sequence of HLA class II genes.Amino acid comparative analysis between SLA class II genes and HLA class II genes were exhibited a high degree of overall identify to the HLA-DRB, -DRA, -DQA, and -DNA molecules (75%, 87%, 86%, 83% identities, respectively). The analysis of SLA genes is important to develop a minipig to induction of HLA genes for conducting forthcoming xenotransplant donor.