Syntheses of Metal Complexes for Diagnosis and Imaging of Tumor Tissue

用于肿瘤组织诊断和成像的金属配合物的合成

• 批准号: 63470071
• 负责人: OGOSHI Hisanobu
• 金额: $ 4.29万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63470071/
• 关键词: Diagnosis of Tumor; Imaging Prove; Metal Complex Accumulated in Tumor; Metalloporphyrin; ^<19>F-nmr; 画像化プロ-ブ; 画像化プローブ

Selective accumulation of water-soluble porphyrin derivatives to tumor or cancer tissue have provided tool for diagnosis and therapy of earlier cancer with aid of photochemical method. However, application of this method is limited to diagnosis of superfacial tumor. Therefore, more elablrate chemical proves are required to detect the position of deep tumor from tissue surface and to make its three dimensional imaging. The first approach is incorporation of radioactive element in chemical prove selectively bound to the tumor tissue. Iodination of deuteroporphyrin was carried our by UV-irradiation of the mixture of porphyrin and iodine.Present method is available to prepare particular porphyrin by using iodine highly diluted with radiocative one. Both fluorescence and photographic measurements can be applied to detect tumor in the deep place of tissues.The second chemical prove is use of fluorinated dyes which are not found in mammalian tissues. ^<19>F-NMR spectra of the fluorinated proves in tissues are observable in high sensitivity. Isotropic shifts of ^<19>F-resonance are also measurable for the paramagnetic metal complexes of perfluoroalkyl- substituted porphyrins. Water soluble fluorinated porphyrins and their metal complexes are readily obtained by new synthetic method. Fluorinated dyes and their metal complexes are readily obtained by new synthetic method. Fluorinated dyes and complexes enable us to detect of tumor location and to define its size by photochemical method, electron spin resonance, and NMR spectroscopy.

水溶性卟啉衍生物在肿瘤或癌组织中的选择性积累，为光化学方法对早期癌症的诊断和治疗提供了工具。然而，该方法的应用仅限于面部表面肿瘤的诊断。因此，从组织表面检测深部肿瘤的位置，并对其进行三维成像，需要更精细的化学证明。第一种方法是将放射性元素在化学物质中选择性结合到肿瘤组织中。用紫外光照射卟啉和碘的混合物，对去卟啉进行了碘化。本方法是用放射性碘高度稀释的碘制备特定卟啉的方法。荧光和照相测量都可以用于检测组织深处的肿瘤。第二个化学证明是使用哺乳动物组织中没有的氟化染料。^<19 b>的F-NMR光谱在组织中被高灵敏度观察到。对于全氟烷基取代卟啉的顺磁性金属配合物，也可测量到^<19> f共振的各向同性位移。用新的合成方法制备了水溶性氟化卟啉及其金属配合物。用新的合成方法制备了含氟染料及其金属配合物。氟化染料和配合物使我们能够通过光化学方法、电子自旋共振和核磁共振光谱来检测肿瘤的位置和确定其大小。

项目成果

K.Aoyagi,H.Toi,Y.Aoyama and H.Ogoshi: "Facile Syntheses of Perfluoroalkyporphyrins Electron Deficient Porphyrin II." Chemistry Letters.29. 1891-1894 (1988)

K.Aoyagi、H.Toi、Y.Aoyama 和 H.Ogoshi：“全氟烷基卟啉缺电子卟啉 II 的简易合成”。

K. Aoyagi, H. Toi, Y. Aoyama and H. Ogoshi: "Facile Syntheses of Perfluoroalkylporphyrin Electron Deficient Porphyrin II." Chemistry Letters, 29, 1891-1894.

K. Aoyagi、H. Toi、Y. Aoyama 和 H. Ogoshi：“全氟烷基卟啉缺电子卟啉 II 的简易合成”。

Y.Aoyama;A.Yamagishi;M.Asagawa;H.Toi;H.Ogoshi: J.Am.Chem.Soc.101. 4076-4077 (1988)

Y.Aoyama；A.Yamagishi；M.Asakawa；H.Toi；H.Ogoshi：J.Am.Chem.Soc.101。

Y.Ozaki,K.Iriyama,H.Ogoshi and T.Kitagawa: "Characterization of Metalloporphyrins and Metallochlorins;Resonance Raman Studies." Nippon Kagaku Kaishi.No.4. 550-557 (1988)

Y.Ozaki、K.Iriyama、H.Ogoshi 和 T.Kitakawa：“金属卟啉和金属二氢卟酚的表征；共振拉曼研究”。

A.Suzuki,K.Okuda,Y.Kamiyama,H.Toi,Y.Aoyama and H.Ogoshi: "Preparation and Properties of Reconstituted Myoglobins with Chemically Modified Hemes." Nippon Kagaku Kaishi.No.4. 505-511 (1988)

A.Suzuki、K.Okuda、Y.Kamiyama、H.Toi、Y.Aoyama 和 H.Ogoshi：“化学修饰血红素重构肌红蛋白的制备和特性”。