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微生物細胞捕捉機能を有する高分子材料の合成及びバイオリアクタ-への応用

具有微生物细胞捕获功能的高分子材料的合成及其在生物反应器中的应用

基本信息

批准号：
63470100
负责人：
KAWABATA Nariyoshi
金额：
$ 4.16万
依托单位：
KYOTO INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

The head investigator has found a new functional polymer, insoluble pyridiniumtype resin, which captures microorganisms alive. The purpose of this research project was to develop a new method of immobilization of microbial cells based on the resin, and to apply for bioreactor. In academic year of 1988, whole cells of Escherichia coli with aspartase activity were immobilized by capture on the surface of the resin. When a suspension of the bacterial cells in buffer solution was passed through a glass column containing beads of the resin, the cells were captured on the surface and formed an immobilized cell system, which was used for the preparation of L-aspartic acid from-ammonium fumarate. Continuous operation of a fixed-bed column reactor containing the unmobilized . bacterial cells produced L-aspartic acid in a quantitative yield when the influent substrate concentration was 0.1 M and the flow rate was 0.41-0.83 bed volumes per hour at pH 7.4-7.7 at 30ﾟC. In academic year of 1989, cells of Arthrobacter simplex having 3-ketosteroid-DELTA^1-dehydrogenase activity were immobilized by capture on the surface of unwoven cloth coated by pyridinium-type polymer. The captured cells catalyzed the transformation of cortisol to prednisolone in methanolic solvents. Continuous operation of a fixed-bed column reactor gave prednisolone in about 90% yield when the influent cortisol concentration was 1.0 mmol/1 and the flow rate was 0.57 bed volumes per hour at 20ﾟC and at pH 8.2.

首席研究员发现了一种新的功能聚合物，不溶性吡啶树脂，它可以捕获活的微生物。本研究旨在开发一种基于树脂的微生物细胞固定化新方法，并应用于生物反应器。在1988学年，通过在树脂表面上的捕获，固定化具有脱氢酶活性的大肠杆菌的全细胞。当细菌细胞在缓冲溶液中的悬浮液通过含有树脂珠的玻璃柱时，细胞被捕获在表面上并形成固定化细胞系统，该固定化细胞系统用于从富马酸铵制备L-天冬氨酸。固定床塔式反应器的连续操作。当进水底物浓度为0.1M，流速为0.41-0.83床体积/小时，pH为7.4-7.7，温度为30 ℃时，细菌细胞以定量产率产生L-天冬氨酸。1989学年，将具有3-酮甾体-DELTA ^1-脱氢酶活性的简单节杆菌细胞捕获固定在涂有吡啶类聚合物的无纺布表面。捕获的细胞在甲醇溶剂中催化皮质醇转化为泼尼松龙。在20 ℃、pH8.2条件下，当进水皮质醇浓度为1.0 mmol/l、流速为0.57床体积/h时，连续操作固定床反应器可得到约90%的泼尼松龙产率。