Endocrinological Study on Phylogeny of Amphibia

两栖类系统发育的内分泌学研究

• 批准号: 63480026
• 负责人: ISHII Susumu
• 金额: $ 4.1万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480026/
• 关键词: Gonadotropin; pituitary gland; antiserum; receptor; amphibia; Bufo japonicus; Rana catesbeiana; evolution; アフリカツメガエル; 分子構造

1) We isolated two species of glycoproteins from pituitary glands of the toad, Bufo japonicus, and identified them as follicle stimulating hormone (FSH) and luteinizing hormone (LH) from their biological activities. Using antisera raised against these hormones, we examined cross reactivity of gonadotropins of various vertebrates. Bullfrog gonadotropins showed relatively high crossreactivity, but mammalian and avian gonadotropins did slightly. Teleostean gonadotropins did not cross-react completely.2) The testicular gonadotropin receptor in the bullfrog, Rana catesbeiana, bound both bullfrog FSH and bullfrog LH with the same affinity when they were tested independently. However, under the presence of both hormone, the binding affinity of LH was in creased as a function of the concentration of FSH. This positive cooperative mechanism suppresses partially the competitive inhibition of LH action by FSH.3) The proline content in the beta-subunit of LH varied among classes of vertebrate vigorously, from about 6% to about 20%, while that of FSH was varied in a small range or almost constant. The amphibian LH beta-subunit showed the smallest content. The positions of the proline residues are considered to be mostly conserved throughout the course of the vertebrate evolution.

1)从蟾蜍脑垂体中分离得到两种糖蛋白，经生物学活性鉴定为促卵泡激素（FSH）和促黄体生成激素（LH）。使用抗血清对这些激素，我们研究了各种脊椎动物的促性腺激素的交叉反应。牛蛙促性腺激素表现出相对较高的交叉反应性，但哺乳动物和鸟类促性腺激素略有。硬骨鱼促性腺激素没有完全交叉反应。2）牛蛙睾丸促性腺激素受体与牛蛙FSH和LH的结合亲和力相同。但在两种激素同时存在下，LH的结合亲和力随FSH浓度的增加而增加。3）LH β-亚基中脯氨酸含量在脊椎动物中变化很大，从6%到20%，而FSH的脯氨酸含量变化很小或几乎不变。两栖类LH β亚基含量最低。脯氨酸残基的位置被认为在整个脊椎动物进化过程中大部分是保守的。

项目成果

Kikuchi,M.: "Radioiodination of chiken luteinizing hormone without affecting receptor binding potency." Biology of Reproduction. 41. 1047-1054 (1989)

Kikuchi,M.：“对鸡黄体生成素进行放射性碘化，而不影响受体结合效力。”

Noce,T.: "Molecular cloning and nucleotide sequence analysis of the putative cDNA for the precursor molecule of the chiken LHーβ subunit." Journal of Molecular Endocrinology. 3. 129-137 (1989)

Noce, T.：“鸡 LH-β 亚基前体分子的假定 cDNA 的分子克隆和核苷酸序列分析。”《分子内分泌学杂志》3. 129-137 (1989)。

Ishii,S.: "Evolution of gonadotropin receptors" Progress in Endocrinolgy 1988,International Congress Series 799,Excepta Medica. 2. 233-238 (1988)

Ishii,S.：“促性腺激素受体的进化”内分泌学进展 1988，国际大会系列 799，Excepta Medica。

Itoh, M.: "Changes in plasma levels of gonadotropins and sex steroids in the toad, Bufo japonicus, in association with behavior during the breeding season" Gen. Comp. Endocrinol. (1990).

Itoh, M.：“蟾蜍（Bufo japonicus）血浆中促性腺激素和性类固醇水平的变化与繁殖季节的行为有关”Gen. Comp。

Kikuchi, M.: "Radioiodination of chicken luteinizing hormone without affecting receptor binding potency" Biol. Reprod. 41 1047-1054 (1989).

Kikuchi, M.：“鸡黄体生成素的放射性碘化而不影响受体结合效力”Biol。