Phylogenetic Analysis of Coryneform and Related Bacteria Based on 5s rRNA Base Sequences

基于5s rRNA碱基序列的棒状杆菌及相关细菌的系统发育分析

• 批准号: 63480057
• 负责人: SUGIYAMA Junta
• 金额: $ 3.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480057/
• 关键词: coryneform bacteria; nocardioform bacteria; Mycobacterium; phylogeny; molecular evolution; chemotaxonomy; 系統分類学; 化学分類学; 5S rRNA; Rhodococcus; Arthrobacter; Tsukamurella; 5S rRNA塩基配列

The generic concept of coryneform bacteria ia mainly based on their chemotaxonomic characteristics such as peptidoglycan structure of cell wall, menaquinone systems, cellular fatty acid composition and DNA base composition, To evaluate the taxonomic system mentioned above, phyligenetic analysis with base sequence of 5s rRNA was carried ou. According to the method of Hori and Osawa, the sequences of eleven strains of coryneform and the related organisms are compared with the previous study by Park et al. Although the genera Phodococcus, Nocardia, and Mycobacterium share the same cell wall structucture containing meso-diaminopimelic acid, arabinogalactan, and mycolic acids with the genus Corynebacterium, the former genera were included in the subgroup proposed by Pard et al. which comprised coryneform bacteria with non-mesodiaminopimelic acid peptidoglycan. Corynebacterium and Brevibacterium constructed subgroups independently.It has been a problem of the taxonomy of coryneform bacteria that the authentic strains are not sufficient. We collected unidentified coryneform bacteria and chemotaxonomically characterized. It was revealed that they included rarely isolated coryneform bacteria such as Tsukamurera and the Arthrobacter nicotianae group. These strains are expected to contribute to the improvement of the taxonomy of coryneform bacteria.

棒状杆菌属的划分主要依据其化学分类学特征，如细胞壁的肽聚糖结构、甲基萘醌系统、细胞脂肪酸组成和DNA碱基组成。根据Hori和Osawa的方法，将11株棒状杆菌和相关生物的序列与Park等人的先前研究进行了比较。虽然Phodococcus属、Nocardia属和Mycobacterium属与棒状杆菌属具有相同的细胞壁结构，含有meso-二氨基庚二酸、阿拉伯半乳聚糖和分枝菌酸，前一种属包括在Pard等提出的亚组中，该亚组包括具有非内二氨基庚二酸肽聚糖的棒状细菌。棒杆菌属和短杆菌属各自独立构成亚群，但由于缺乏可靠的菌株，一直是棒杆菌属细菌分类的难题。我们收集了身份不明的棒状细菌和化学分类学的特点。结果表明，它们包括很少分离的棒状细菌，如Tsukamurera和烟草节杆菌群。这些菌株有望为棒状细菌分类学的改进做出贡献。

项目成果

Y.H.Park,H.Hori,K.Suzuki,J.Sugiyama and K.Komagata: "Further phylogenetic analysis of some coryneform and nocardioform bacteria by 5S rRNA" Journal of General and Applied Microbiology,in preparation.

Y.H.Park、H.Hori、K.Suzuki、J.Sugiyama 和 K.Komagata：“通过 5S rRNA 对一些棒状杆菌和诺卡氏菌进行进一步的系统发育分析”普通与应用微生物学杂志，正在准备中。

F. Yanagida, Y. Niimura, E. Koh, K. Suzuki, M. Kozaki and K. Komagata: "Nucleotide sequence of 5S ribosomal RNAs of 'Amphibacterium xylianus' and Clostridium carnis" Nucleic Acid Research. 17 :. 443 (1989)

F. Yanagida、Y. Niimura、E. Koh、K. Suzuki、M. Kozaki 和 K. Komagata：“‘木双杆菌’和肉梭菌 5S 核糖体 RNA 的核苷酸序列”核酸研究。

J. S. Park, H. Hori and K. Komagata.: "Nucleotide sequences of 5S ribosomal RNAs of Planococcus citreus, Planococcus kocurii and Sporosarcina ureae" Nucleic Acid Research. 16 :. 10358 (1988)

J. S. Park、H. Hori 和 K. Komagata.：“柑橘平球菌、kocurii 平球菌和脲孢八叠球菌 5S 核糖体 RNA 的核苷酸序列”核酸研究。

Y. H. Park, H. Hori, K. Suzuki, J. Sugiyama and K. Komagata: "Further phylogenetic analysis of some coryneform and nocardioform bacteria by 5S rRNA" Journal of General and Applied Microbiology,.

Y. H. Park、H. Hori、K. Suzuki、J. Sugiyama 和 K. Komagata：“通过 5S rRNA 对一些棒状杆菌和诺卡氏菌进行进一步的系统发育分析”普通与应用微生物学杂志。

F.Yanagita,Y.Niimura,E.Koh,K.Suzuki,M.Kozaki andK.Komagata: "Nucleotide sequence of 5S ribosomal RNAs of ′Amphibacillus xylanus′ and Clostridium carnis" Nucleic Acid Research. 17. 443- (1989)

F. Yanagita、Y. Niimura、E. Koh、K. Suzuki、M. Kozaki 和 K. Komagata：“‘Amphibacillus xylanus’ 和 Clostridium carnis 的 5S 核糖体 RNA 的核苷酸序列”核酸研究 17. 443- (1989)。 ）