The Trial of Human Coronary Artery Endothelial Cell Culture.

人冠状动脉内皮细胞培养的试验。

• 批准号: 63480197
• 负责人: MURASE Toshio
• 金额: $ 4.1万
• 依托单位: Okinaka Memorial Institute for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480197/
• 关键词: coronary artery sclerosis; Endothelial cells; Cell culture

The aim of our study is to establish the culture system of human coronary artery endothelial cells, because the establishment is indispensable for the studies of the pathogenesis of coronary artery sclerosis. We have done many trials on autopsied materials. Since coronary arteries from younger subjects without apparent coronary artery sclerosis were thought to be the appropriate materials for this purpose, we examined several fetus materials as well.With all our efforts, we have not yet succeeded in the culture of coronary artery endothelial cells. Now we are doing more than we did the culture trials on human materials. We are also doing preliminary studies using porcine coronary arteries.To our knowledge, there have been no reports showing the successful establishment of human coronary artery endothelial cell culture.

我们的研究目的是建立人冠状动脉内皮细胞培养体系，因为这是研究冠状动脉硬化发病机制必不可少的。我们对尸体解剖过的材料做过很多试验。由于没有明显冠状动脉硬化的年轻受试者的冠状动脉被认为是合适的材料，我们也检查了几个胎儿的材料。经过我们的努力，冠状动脉内皮细胞的培养还没有成功。现在我们做的不仅仅是人体材料的培养试验。我们也在用猪冠状动脉做初步研究。据我们所知，还没有报道显示成功建立人冠状动脉内皮细胞培养。

项目成果

M.Kawakami,Y.Kondo,Y.Imai,M.Hashiguchi,H.Ogawa,A.Hiragun,S.Aotsuka,S.Shibata,T.Oda,T.Murase and F.Takaku: "Suppression of lipoprotein lipase in 3T3ーL1 cells by a mediator produced by SEKI melanoma,a cachexiaーinducing human melanoma cell line." J.Biochem.1

M. Kawakami、Y. Kondo、Y. Imai、M. Hashiguchi、H. Okawa、A. Hiragun、S. Aotsuka、S. Shibata、T. Oda、T. Murase 和 F. Takaku：“脂蛋白脂肪酶的抑制3T3-L1 细胞由 SEKI 黑色素瘤（一种诱导恶病质的人类黑色素瘤细胞系）产生的介质产生。”J.Biochem.1

T.Murase,M.Okubo and M.Hara: "Does chylomicronemia cause atherosclerosis?" Tohoku J.Exp.Med.

T.Murase、M.Okubo 和 M.Hara：“乳糜微粒血症会导致动脉粥样硬化吗？”

Keiko Kawakami: "A rapid electrophoretic method for the detection of serum Lp(a) lipoprotein" Clin Chim Acta. 185. 147-156 (1989)

Keiko Kawakami：“一种检测血清 Lp(a) 脂蛋白的快速电泳方法”Clin Chim Acta。

K. Nakanishi, T. Kobayashi, H. Miyashita, M. Okubo, T. Sugimoto, T. Murase, K. Kosaka, K. Inoue and M. Kono: "Relationships among islet cell autoantibodies, residual beta-cell function, and metabolic control in patients with insulin-dependent diabetes mel

K. Nakanishi、T. Kobayashi、H. Miyashita、M. Okubo、T. Sugimoto、T. Murase、K. Kosaka、K. Inoue 和 M. Kono：“胰岛细胞自身抗体、残余 β 细胞功能和胰岛素抵抗之间的关系

Takanari Gotoda: "Detection of familial LPL deficiency by PvuII RFLP" Nucleic Acids Research. 17. 3607 (1989)

Takanari Gotoda：“通过 PvuII RFLP 检测家族性 LPL 缺乏”核酸研究。