The Trial of Human Coronary Artery Endothelial Cell Culture.

本研究的目的是建立人冠状动脉内皮细胞的培养体系，因为这一体系的建立对于研究冠状动脉硬化的发病机制是必不可少的。我们在尸检材料上做了很多试验。由于没有明显冠状动脉硬化的年轻受试者的冠状动脉被认为是适合这一目的的材料，我们也检查了几种胎儿材料。尽管我们努力了，但冠脉内皮细胞的培养尚未成功。现在，我们所做的比在人体材料上进行的培养试验更多。我们还在利用猪冠状动脉进行初步研究。据我们所知，还没有报告表明成功地建立了人冠状动脉内皮细胞培养。

The aim of our study is to establish the culture system of human coronary artery endothelial cells, because the establishment is indispensable for the studies of the pathogenesis of coronary artery sclerosis. We have done many trials on autopsied materials. Since coronary arteries from younger subjects without apparent coronary artery sclerosis were thought to be the appropriate materials for this purpose, we examined several fetus materials as well.With all our efforts, we have not yet succeeded in the culture of coronary artery endothelial cells. Now we are doing more than we did the culture trials on human materials. We are also doing preliminary studies using porcine coronary arteries.To our knowledge, there have been no reports showing the successful establishment of human coronary artery endothelial cell culture.