Experimental Study of the Influence of Intraperitoneal Thermochemotherapy on the Healing of Intestinal Anastomoses

当手术后在腹膜腔内应用热疗时，询问它是否会干扰随后的肠吻合口的愈合是很重要的。为研究这一问题，制备了大鼠肠吻合模型，并将其分为3组。A组：局部热疗(44ﾟC×30min)。应用于使用生理盐水进行吻合术的肠环。B组：局部热疗(44ﾟC×30min)。用生理盐水加丝裂霉素C(MMC；10 mg/L)。对照组不进行任何热处理。各组大鼠分别于术后不同时间处死动物，通过断裂强度测定和组织学检查评价吻合口愈合情况。术后第3天，3组吻合口断裂强度均降至最低值，但无统计学差异。在第7天或第14天，3组动物的抗断裂能力都有所增强，其中以热处理组最强。组织学发现支持这些结果。然而，热疗组手术后恢复正常体重的时间明显延迟。因此，局部热疗应控制在44.0min C×30min的范围内。对肠吻合口愈合无不良影响。

When hyperthermia is applied in the peritoneal cavity after surgery, it is important to ask whether or not it interferes with the subsequent healing of intestinal anastomoses. To study this problem, rats with intestinal anastomoses were prepared and divided into 3 groups. In group A, local hyperthermia (44.0ﾟC x 30 min.) was applied to the intestinal loop that containes anastomoses using saline. in group B, local hyperthermia (44.0ﾟC x 30 min.) was applied using saline supplemented with Mitomycin C (MMC; 10 mg/L). In the control group, no thermal treatment was applied. In each groups, rats were sacrificed at various times after surgery, and anastomotic healing was assessed by measurement of breaking strength and histological examination. On the 3rd day after operation, the breaking strength of anastomoses in these 3 groups decreased to the lowest values, but no statistical differences were noted. On the 7th day or 14th day, increased resistance to breaking developed in all 3 groups and was greatest in the thermally treated groups. Histological findings supported these results. However, restoration of normal body weight after surgery was significantly delayed in the thermally treated groups. Thus, it appears that local hyperthermia within the limits of 44.0ﾟC x 30 min. had no adverse effects on the healing of intestinal anastomoses.