Experimental Study of the Influence of Intraperitoneal Thermochemotherapy on the Healing of Intestinal Anastomoses

当手术后腹腔内应用高温时，重要的是要询问它是否会干扰肠道疾病的后续愈合。为了研究这个问题，制备了具有肠道疾病的大鼠并将其分为3组。A组采用局部热疗（44.0 ℃ × 30 min.）使用盐水将其应用于含有乳糖的肠环。B组采用局部热疗（44.0 ℃ × 30 min.）使用补充有丝裂霉素C（MMC; 10 mg/L）的盐水施用。对照组不进行热处理。各组在术后不同时间处死大鼠，通过测量断裂强度和组织学检查评估吻合口愈合。术后第3天，3组骨的断裂强度均降至最低值，但差异无统计学意义。在第7天或第14天，在所有3个组中产生了增加的抗断裂性，并且在热处理组中最大。组织学结果支持这些结果。然而，在热处理组中，手术后恢复正常体重显著延迟。因此，在44.0 ° C x 30 min范围内的局部高温似乎对肠坏死的愈合没有不良影响。

When hyperthermia is applied in the peritoneal cavity after surgery, it is important to ask whether or not it interferes with the subsequent healing of intestinal anastomoses. To study this problem, rats with intestinal anastomoses were prepared and divided into 3 groups. In group A, local hyperthermia (44.0ﾟC x 30 min.) was applied to the intestinal loop that containes anastomoses using saline. in group B, local hyperthermia (44.0ﾟC x 30 min.) was applied using saline supplemented with Mitomycin C (MMC; 10 mg/L). In the control group, no thermal treatment was applied. In each groups, rats were sacrificed at various times after surgery, and anastomotic healing was assessed by measurement of breaking strength and histological examination. On the 3rd day after operation, the breaking strength of anastomoses in these 3 groups decreased to the lowest values, but no statistical differences were noted. On the 7th day or 14th day, increased resistance to breaking developed in all 3 groups and was greatest in the thermally treated groups. Histological findings supported these results. However, restoration of normal body weight after surgery was significantly delayed in the thermally treated groups. Thus, it appears that local hyperthermia within the limits of 44.0ﾟC x 30 min. had no adverse effects on the healing of intestinal anastomoses.