The Influence of Limb Immobilization and Exercise on the Structure and Function of Mouse Soleus Muscle

肢体固定和运动对小鼠比目鱼肌结构和功能的影响

• 批准号: 63480338
• 负责人: TACHINO Katsuhiko
• 金额: $ 3.26万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480338/
• 关键词: muscle atrophy; hindlimb suspension; muscle fiber type; motor endplate; morphometry; treadmill running; 廃用性萎縮; 脱神経; 筋よりの誘導物質; 癈用性筋萎縮; 収縮蛋白質の変性; SDS-PAGE; 軸索輸送; 分子量65,000; 筋萎縮; 神経発芽; 神経軸索輸送

This study was undertaken to determine the amelioration effect of a daily exercise on disuse atrophy of mouse soleus induced by hindlimb suspension. Physiologic, histologic and morphometric examination were made on mice were divided into control (CON), hindlimb suspension (HS) and HS plus exercise (HS-EX) with daily treadmill running. Immediately after checking the contractile function of this muscle, Adenosine triphosphatase staining was employed to classify into type 1 or 2 fibers. The ultrstructure of the cytoplasmic organelles of both fibers and their motor endplates were studied and compared morphometrically or qualitively. The soleus muscle activity was reduced to below 2/3 on HS, but increased to over 1.4 times of its normal activity on HS-EX. Maximum twitch and tetanic tensions in the HS group were less than those in CON, with those HS-EX being significantly greater than those in HS. HS reduced the size of both type 1 and 2 fibers and and increased the percentage of type 1 fibers, but not in the HS-EX muscles. Atrophy of a few myofibrills was observed after HS-EX, wheras after HS many myofibrills showed degeneration of the sarcomere of both 1 and 2 fibers. Endplates in the HS group were found to exhibit a great deal of ultrastructual evidence of degeneration and regeneration, but those in the HS-EX group were less affected. The endplates in 2 fibers exhibited structural alteration to a greater extent than type 1 endplates in HS alone. This suggests that type 1 endplates were more resistant to degenerative changes than the 2 fibers. The mitochondrial volume fraction after HS decreased both in the 1 and 2 fibers, whereas it diminished only in the 2 fibers HS-EX as compared with the values in the CON. These data show that exercise training can markedly attenuate the detrimental effects of HS on the soleus muscle.

本研究旨在探讨日常锻炼对后肢悬吊所致小鼠比目鱼肌废用性萎缩的改善作用。将小鼠分为对照组(CON)、后肢悬吊组(HS)和HS+运动组(HS-EX)进行生理、组织学和形态计量学检查。在检查该肌肉的收缩功能后，立即用三磷酸腺苷酶染色将其分为1或2型纤维。对这两种纤维及其运动终板的细胞质细胞器的超微结构进行了形态计量学或定性比较。HS组比目鱼肌活动降低到2/3以下，HS-EX组则增加到正常活动的1.4倍以上。HS组的最大抽动张力和强直性张力低于CON组，HS-EX显著大于HS组。HS减小了1型和2型纤维的大小，增加了1型纤维的百分比，但在HS-EX肌肉中没有。HS-EX后少数肌原纤维萎缩，HS后大量肌原纤维1、2肌节变性。HS组的终板有大量变性和再生的超微结构证据，而HS-EX组的终板受影响较小。2个纤维中的终板结构改变比单纯HS中的1型终板更大。这表明，1型终板比2型终板更能抵抗退行性改变。与CON相比，HS后1、2纤维线粒体体积分数均减少，而仅HS-EX减少。这些数据表明，运动训练可以显着减轻HS对比目鱼肌的不利影响。

项目成果

立野 勝彦: "部分脱神経筋に対する運動負荷の実験的研究" 運動療法研究会論文集. 14. 28-30 (1990)

Katsuhiko Tateno：“部分去神经肌肉运动负荷的实验研究”运动治疗研究组论文集 14. 28-30 (1990)。

灰田 信英: "運動療法の科学的基礎ー末梢神経・筋障害を中心としてー" 理学療法ジャ-ナル. 23. 203-209 (1989)

Nobuhide Haida：“运动疗法的科学基础 - 关注周围神经和肌肉疾病”物理治疗杂志 23. 203-209 (1989)。

立野 勝彦・他: "部分脱神経筋に対する運動負荷の実験的研究" 運動療法研究会論文集. 14. 28-30 (1990)

Katsuhiko Tateno 等人：“部分去神经肌肉运动负荷的实验研究”运动治疗研究组论文集 14. 28-30 (1990)。

Fujiko Someya;Katsuhiko Tachino;Nobuhide Haida: Brain Research.

染谷不二子；立野克彦；灰田信秀：大脑研究。

灰田 信英: "マウスヒラメ筋の廃用性萎縮の病態ならびに運動負荷効果に関する研究" 十全医学会雑誌. 100. 1-18 (1991)

Nobuhide Haida：“小鼠比目鱼肌废用性萎缩的病理学和运动负荷的影响的研究”十善医学会杂志 100. 1-18 (1991)。