Histamine and polyamine changes in tissue development of tongue and drugs effects.

本研究的目的是阐明组胺和多胺在组织发育中的作用。因此，本实验研究了舌癌细胞种植或DYBA（9，10-dimethyl-1，2-benzanthracene）诱发舌癌后，舌组织中这些胺水平和组氨酸脱羧酶（HDC）活性的变化.用肥大细胞缺陷型W/W^v小鼠舌内植入埃利希腹水瘤细胞后，观察肿瘤生长和组织胺含量的变化，并与同基因+/+小鼠和正常ddY小鼠作比较。W/W_v小鼠的肿瘤体积小于+/+或ddY小鼠。植入后6天+/+和ddY组舌组胺含量下降，而W/W^v组舌组胺含量上升。提示肥大细胞和组胺在组织生长过程中起重要作用，尤其在早期阶段。我们正在研究组胺的作用，使用-FMH，一个HDC的附件。将DMBA涂于金黄地鼠舌上，测定HDC和ODC（鸟氨酸脱羧酶）活性变化。DMBA处理可诱导HDC和ODC活性的增加，而放线菌素D处理仅显著提高HDC活性。这些结果提示，DMBA涂抹后HDC活性的快速增加可能是由于舌内非肥大细胞中诱导型HDC的产生.本文观察了DMBA诱发金黄地鼠舌癌模型中组胺和多胺含量的变化。多胺含量显著增加，X射线照射使多胺含量降低，而组胺含量无明显变化，提示组胺在组织发育的早期阶段，特别是舌肿瘤的发生发展中起重要作用。

The aim of this investigation was to clarify the role of histamine and polyamines in tissue development. So, we studied on the changes of these amines levels and activity of histidine decarboxylase ( HDC ) in the tongue tissues after implantation of tumor cells or provocation of tongue carcinoma induced by DYBA ( 9,10-dimethyl-1,2-benzanthracene ).1. W/W^v mice, genetically mast cell deficient, was employed to examine the changes of tumor growth and histamine content in early stages after implantation of Ehrlich ascites cells in the tongue and compared with the changes in those of congenic +/+ mice or normal ddY mice. The tumor volume in W/W^v mice was smaller than +/+ or ddY at the same time. Histamnine contents in the tongues of +/+ and ddY decreased 6 day after implantation but in W/W^v it increased. These results suggest that mast cells and histamine play an important role in tissue growing process, especially in early stages. We are investigating on the histamine roles using -FMH, a HDC inhibitor.2. DMBA was painted on the tongue of golden hamster and changes of activities of HDC and ODC ( ornithine decarboxylase ) were estimated. Application of DMBA induced the increases of HDC and ODC activities, but, when applicated with Actinomycin D, only HDC activity was markedly enhanced. These results suggest that rapid increase of HDC activity by painting with DMBA may be due to the induction of " inducible HDC " in non-mast cells in tongue.3. The changes of histamine and polyamine contents in the hamster tongue carcinoma induced by DMBA painting were estimated. There was significant increase of polyamine contents and X-ray radiation decreased the contents of polyamines, but histamine content was not changed.In this investigated, we got the results suggesting that histamine plays an important role in early stages of tissue development, especially in tumor development in tongue.