Transgenerational phenotypic plasticity: Cardiorespiratory physiology of the parthenogenetic marbled crayfish as a model system

跨代表型可塑性：孤雌大理石纹小龙虾作为模型系统的心肺生理学

• 批准号: 438130889
• 负责人: Dr. Torben Göpel
• 金额: --
• 依托单位: Department of Biological Sciences
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/438130889?language=en
• 关键词: Transgenerational; phenotypic; plasticity; Cardiorespiratory; physiology

The aim of this project is to investigate phenotypic plasticity (i.e. the formation of different phenotypes based on the same genotype) of physiological parameters of the cardiorespiratory system. It is hypothesized that phenotypic plasticity might represent an interim solution until evolutionary adaptation can take place. Thus, it can be regarded as important evolutionary phenomenon. As a model system, physiology of the cardiorespiratory system of the parthenogenetic marbled crayfish, Procambarus fallax f. virginalis, will be studied. This species, of which only females exist, produces genetically identical offspring and thus is well suited for studies on phenotypic plasticity. To trigger effects of phenotypic plasticity, individuals will be exposed to environmental stressors (hypoxia, increased temperature, pollution with polycyclic aromatic carbohydrates) during early development. In later stages, physiological parameters (heart rate, stroke volume, cardiac output, oxygen consumption) will be measured and compared to a control group (raised under optimal conditions). Heart rate, stroke volume and cardiac output will be determined using video sequences. Measurement of oxygen consumption will be done with special measurement chambers with an optical fluorescence oxygen-sensing system. Beside intragenerational effects of phenotypic plasticity, transgenerational inheritance of plastic physiological parameters (as is expected due to knowledge on such effects in other species) will be studied. To that end, the same physiological parameters will be measured in the offspring, which (other than the mother individuals) have not been exposed to the environmental stressors themselves. It shall be shown that phenotypic plasticity of cardiorespiratory physiology is an interesting phenomenon that can lead to adaptation to fast change of environmental conditions.

本项目旨在研究心肺系统生理参数的表型可塑性（即在相同基因型的基础上形成不同表型）。据推测，在进化适应发生之前，表型可塑性可能是一种临时解决方案。因此，它可以被视为一种重要的进化现象。本文将孤雌生殖大理石纹小龙虾原螯虾（Procambarus fallax f. virginalis）的心肺系统生理学作为模型系统进行研究。该物种只有雌性存在，其后代基因相同，因此非常适合研究表型可塑性。为了触发表型可塑性的影响，个体在发育早期会暴露于环境应激源（缺氧、温度升高、多环芳香族碳水化合物污染）。在后期阶段，将测量生理参数（心率，搏量，心输出量，耗氧量）并与对照组（在最佳条件下饲养）进行比较。心率，中风量和心输出量将通过视频序列来确定。耗氧量的测量将通过带有光学荧光氧传感系统的特殊测量室来完成。除了表型可塑性的代际影响外，还将研究塑性生理参数的跨代遗传（由于对其他物种的这种影响的了解，这是预期的）。为了达到这个目的，同样的生理参数将在后代身上测量，这些后代（除了母亲个体）本身没有暴露在环境压力源中。应该表明，心肺生理学的表型可塑性是一种有趣的现象，它可以导致适应环境条件的快速变化。

项目成果

Cores of Reproducibility in Physiology: Insufficient Reporting of Experimental Variables as a Cause for Non-Reproducibility in Animal Physiology? A Case Study.

生理学可重复性的核心：实验变量报告不充分是动物生理学不可重复性的原因？

• DOI: 10.1152/ajpregu.00026.2022
• 期刊: American journal of physiology. Regulatory, integrative and comparative physiology
• 作者: Göpel;Burggren
• 通讯作者: Burggren

High degree of non-genetic phenotypic variation in the vascular system of crayfish: a discussion of possible causes and implications

小龙虾血管系统的高度非遗传表型变异：可能原因和影响的讨论

• DOI: 10.1007/s00435-021-00536-2
• 期刊: Zoomorphology
• 影响因子: 1
• 作者: Scholz;Göpel;Richter;Wirkner
• 通讯作者: Wirkner