Confocal Fluorescence Microscope with superresolution and time-resolved fluorescence module

必须采购具有高空间和时间分辨率的共聚焦荧光显微镜，并将成为鲁道夫-维尔乔中心中央成像设施的一部分，该中心在维尔茨堡大学校园和大学医院拥有广泛的用户群体。这种新型显微镜将有助于回答活细胞和模式生物的各种生物医学问题，并将促进新方法的发展。所要求的设备计划取代一个12年的，很大程度上技术上过时的共聚焦显微镜（荧光终身成像功能），这仍然被广泛使用的广大观众在校园。由于目前要更换的旧显微镜无法按照制造商的声明进行升级，因此迫切需要采购新的共聚焦荧光显微镜，以保持有关研究小组的科学竞争力。需要新显微镜关注的科学问题，即g蛋白偶联受体的激活动力学，心血管疾病的生理调节过程和发病机制，以及申请人实验室本身的方法开发，这总是使所有用户受益，没有弯路。成像设施由三名高素质的员工提供支持，并将所要求的显微镜完全集成到成像设施的现有设备中，并使仪器始终保持功能，并为所有用户提供最新的设备。

A confocal fluorescence microscope with high spatial and temporal resolution has to be procured, and will be part of the central imaging facility of the Rudolf-Virchow-Center with a broad user community at the campus of the University of Würzburg and the University Hospitals. The new microscope will help to answer various biomedical questions in living cells and model organisms in combination and will facilitate new methodological developments. The requested device is planned to replace a 12-years-old, largely technically obsolete Confocal Microscope (with Fluorescence Lifetime Imaging feature), which is still extensively used by a broad audience at the campus. Since the current, old microscope to be replaced can no longer be upgraded according to the manufacturer's statements, the procurement of a new confocal fluorescence microscope is urgently required in order to preserve the scientific competitiveness of the research groups concerned. The scientific questions that require the new microscope concern, i.a. the activation dynamics of G-protein coupled receptors, the physiological regulatory processes and the pathogenesis of cardiovascular diseases, as well as the method development in the applicant’s laboratory itself, which always benefits all users without detours. The imaging Facility is supported by three highly qualified employees, and will fully integrate the requested microscope into the existing equipment of the Imaging Facility and keep the instrument always functional and up-to-date for all users.