Confocal Fluorescence Microscope with superresolution and time-resolved fluorescence module

必须采购一台具有高空间和时间分辨率的共聚焦荧光显微镜，它将成为Rudolf-Virchow中心中央成像设施的一部分，该中心在维尔茨堡大学校园和大学医院拥有广泛的用户群体。新的显微镜将有助于回答活细胞和模型生物体的各种生物医学问题，并将促进新的方法的发展。所要求的设备计划取代一个有12年历史的、技术上基本过时的共焦显微镜（具有荧光寿命成像功能），该显微镜仍被校园内的广大受众广泛使用。由于现有的旧显微镜无法根据制造商的声明进行升级，因此迫切需要采购新的共焦荧光显微镜，以保持有关研究小组的科学竞争力。需要新的显微镜关注的科学问题，即。G蛋白偶联受体的激活动力学、心血管疾病的生理调节过程和发病机制，以及申请人实验室本身的方法开发，这些都使所有用户受益而不走弯路。成像设施由三名高素质的员工提供支持，并将所需的显微镜完全集成到成像设施的现有设备中，并为所有用户保持仪器始终功能和最新状态。

A confocal fluorescence microscope with high spatial and temporal resolution has to be procured, and will be part of the central imaging facility of the Rudolf-Virchow-Center with a broad user community at the campus of the University of Würzburg and the University Hospitals. The new microscope will help to answer various biomedical questions in living cells and model organisms in combination and will facilitate new methodological developments. The requested device is planned to replace a 12-years-old, largely technically obsolete Confocal Microscope (with Fluorescence Lifetime Imaging feature), which is still extensively used by a broad audience at the campus. Since the current, old microscope to be replaced can no longer be upgraded according to the manufacturer's statements, the procurement of a new confocal fluorescence microscope is urgently required in order to preserve the scientific competitiveness of the research groups concerned. The scientific questions that require the new microscope concern, i.a. the activation dynamics of G-protein coupled receptors, the physiological regulatory processes and the pathogenesis of cardiovascular diseases, as well as the method development in the applicant’s laboratory itself, which always benefits all users without detours. The imaging Facility is supported by three highly qualified employees, and will fully integrate the requested microscope into the existing equipment of the Imaging Facility and keep the instrument always functional and up-to-date for all users.