Acquisition of confocal scan to upgrade existing fluorescence microscope
获取共焦扫描以升级现有荧光显微镜
基本信息
- 批准号:10796239
- 负责人:
- 金额:$ 23.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-05-01 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAdministrative SupplementBiological MarkersCellsComplexDevelopmentDiseaseEmbryonic DevelopmentEndosomesEquipmentFosteringFutureGenesImageMAPK3 geneMalignant NeoplasmsMediatingMicroscopeModelingMolecularOutcomePathway interactionsResearchRoleScaffolding ProteinScanningSignal TransductionSortingSpecificitySyndromeUbiquitinWorkZebrafishdevelopmental diseaseexperimental studyfluorescence microscopeimprovedin vivoinsightnovel diagnosticsnovel therapeutic interventionprogramspublic health relevancesegregationtargeted treatmenttherapeutic developmenttransmission processtumorvirtual
项目摘要
Project Summary
This proposal is for an equipment administrative supplement to an existing MIRA R35 supporting the PI’s
research addressing the molecular mechanisms of the extracellular signal-regulated kinases 1 and 2 (ERK1/2)
signal transmission as guided by scaffold proteins. This research program focuses on understanding the
mechanisms by which key regulators, such as the scaffold protein Shoc2, modify ERK1/2 signaling. These
studies decipher and determine the mechanisms by which endosomal sorting regulates the ubiquitin-driven
remodeling of Shoc2 complexes and signaling and illuminate the key steps that segregate Shoc2 into distinct
intracellular sorting pathways. Preliminary studies have already yielded new insights into the molecular details
of how Shoc2-activated ERK1/2 signals promote early developmental stages. For this work, zebrafish is used
as a vertebrate model to investigate the in vivo effect of Shoc2 gene editing on the early stages of
development since they offer distinct advantages for studies of development, including their transparency, as
well as external and rapid development. Outcomes from this work are expected to produce an ambitious and
comprehensive mechanistic understanding of how Shoc2 is involved in determining the specificity of ERK1/2
signaling outcomes. As such, this work is laying the groundwork for future studies on developmental disorders
that will contribute to the advancement of novel therapeutic strategies and biomarkers. This administrative
supplement application to acquire a confocal scanning disk as an ‘add-on’ to an existing 3i Marianas
microscope platform will foster ongoing experiments in this research program. The advanced scanning
capacities will significantly improve the quality of the imaging analysis to examine the distribution of Shoc2
signaling complexes in cells and will dramatically enhance these studies to examine the role of Shoc2-
mediated signals in embryonic development.
项目摘要
这项提议是对现有的支持PI的Mira R35的设备管理补充
细胞外信号调节蛋白1和2(ERK1/2)分子机制研究
由支架蛋白引导的信号传递。本研究计划的重点是了解
关键调节者,如支架蛋白Shoc2,修改ERK1/2信号的机制。这些
研究破译和确定内体分选调节泛素驱动的机制
Shoc2复合体和信号转导的重塑,并说明将Shoc2分离为不同的关键步骤
细胞内分选途径。初步研究已经对分子细节产生了新的见解
Shoc2激活的ERK1/2信号如何促进早期发育阶段。在这项工作中,使用了斑马鱼
作为脊椎动物模型来研究Shoc2基因编辑在早期阶段的体内效应
发展,因为它们为发展研究提供了明显的优势,包括其透明度,如
以及外部快速发展。这项工作的成果预计将产生一个雄心勃勃的
全面理解Shoc2如何参与决定ERK1/2的特异性
发出结果信号。因此,这项工作正在为未来对发育障碍的研究奠定基础
这将有助于新的治疗策略和生物标记物的发展。这项管理工作
补充申请以获取共焦扫描盘,作为现有3i Marianas的附加组件
显微镜平台将在这一研究计划中促进正在进行的实验。先进的扫描技术
能力将显著提高检查Shoc2分布的成像分析的质量
细胞内的信号复合体,并将极大地加强这些研究,以检验Shoc2-
胚胎发育中的中介信号。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Emilia Galperin其他文献
Emilia Galperin的其他文献
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{{ truncateString('Emilia Galperin', 18)}}的其他基金
Mechanisms and Functions of Shoc2-Transduced Cellular Signals
Shoc2 转导的细胞信号的机制和功能
- 批准号:
10387097 - 财政年份:2020
- 资助金额:
$ 23.45万 - 项目类别:
Mechanisms and functions of Shoc2-transduced cellular signals
Shoc2 转导的细胞信号的机制和功能
- 批准号:
10395494 - 财政年份:2020
- 资助金额:
$ 23.45万 - 项目类别:
Mechanisms and functions of Shoc2-transduced cellular signals
Shoc2 转导的细胞信号的机制和功能
- 批准号:
10613880 - 财政年份:2020
- 资助金额:
$ 23.45万 - 项目类别:
Diversity Supplement - Deciphering the Molecular Mechanisms Underlying Active Scaffolding
多样性补充 - 破译活性支架背后的分子机制
- 批准号:
9249209 - 财政年份:2015
- 资助金额:
$ 23.45万 - 项目类别:
Deciphering the molecular mechanisms underlying active scaffolding
破译活性支架背后的分子机制
- 批准号:
9279178 - 财政年份:2015
- 资助金额:
$ 23.45万 - 项目类别:
ROLE OF SHOC2 PROTEIN IN SPATIO-TEMPORAL REGULATION OF ERK KINASE CASCADE
SHOC2 蛋白在 ERK 激酶级联时空调控中的作用
- 批准号:
8360579 - 财政年份:2011
- 资助金额:
$ 23.45万 - 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
- 批准号:
8137875 - 财政年份:2010
- 资助金额:
$ 23.45万 - 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
- 批准号:
8114450 - 财政年份:2010
- 资助金额:
$ 23.45万 - 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
- 批准号:
8322198 - 财政年份:2010
- 资助金额:
$ 23.45万 - 项目类别:
Regulation of MAP Activity by EGFR Endocytosis
EGFR 内吞作用对 MAP 活性的调节
- 批准号:
7683858 - 财政年份:2008
- 资助金额:
$ 23.45万 - 项目类别:
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