Acquisition of confocal scan to upgrade existing fluorescence microscope

获取共焦扫描以升级现有荧光显微镜

基本信息

  • 批准号:
    10796239
  • 负责人:
  • 金额:
    $ 23.45万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-05-01 至 2025-04-30
  • 项目状态:
    未结题

项目摘要

Project Summary This proposal is for an equipment administrative supplement to an existing MIRA R35 supporting the PI’s research addressing the molecular mechanisms of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) signal transmission as guided by scaffold proteins. This research program focuses on understanding the mechanisms by which key regulators, such as the scaffold protein Shoc2, modify ERK1/2 signaling. These studies decipher and determine the mechanisms by which endosomal sorting regulates the ubiquitin-driven remodeling of Shoc2 complexes and signaling and illuminate the key steps that segregate Shoc2 into distinct intracellular sorting pathways. Preliminary studies have already yielded new insights into the molecular details of how Shoc2-activated ERK1/2 signals promote early developmental stages. For this work, zebrafish is used as a vertebrate model to investigate the in vivo effect of Shoc2 gene editing on the early stages of development since they offer distinct advantages for studies of development, including their transparency, as well as external and rapid development. Outcomes from this work are expected to produce an ambitious and comprehensive mechanistic understanding of how Shoc2 is involved in determining the specificity of ERK1/2 signaling outcomes. As such, this work is laying the groundwork for future studies on developmental disorders that will contribute to the advancement of novel therapeutic strategies and biomarkers. This administrative supplement application to acquire a confocal scanning disk as an ‘add-on’ to an existing 3i Marianas microscope platform will foster ongoing experiments in this research program. The advanced scanning capacities will significantly improve the quality of the imaging analysis to examine the distribution of Shoc2 signaling complexes in cells and will dramatically enhance these studies to examine the role of Shoc2- mediated signals in embryonic development.
项目摘要 本提案是对现有MIRA R35的设备管理补充,以支持PI 细胞外信号调节激酶1和2(ERK 1/2)的分子机制研究 由支架蛋白引导的信号传输。该研究计划的重点是了解 关键调节因子如支架蛋白Shoc 2修饰ERK 1/2信号传导的机制。这些 研究破译并确定了内体分选调节泛素驱动的 Shoc 2复合物的重塑和信号传导,并阐明了将Shoc 2分离成不同的 细胞内分选途径。初步研究已经对分子细节产生了新的见解 Shoc 2激活的ERK 1/2信号如何促进早期发育阶段。在这项工作中,斑马鱼被用来 作为脊椎动物模型来研究Shoc 2基因编辑对早期阶段的体内影响 因为它们为发展研究提供了独特的优势,包括其透明度, 外部快速发展。这项工作的成果预计将产生一个雄心勃勃的, 对Shoc 2如何参与决定ERK 1/2特异性的全面机制理解 信号结果。因此,这项工作为未来对发育障碍的研究奠定了基础 这将有助于新的治疗策略和生物标志物的发展。这一行政 作为现有3 i Marianas的“附加”,获得共焦扫描盘的补充应用 显微镜平台将促进这项研究计划正在进行的实验。高级扫描 能力将显着提高成像分析的质量,以检查Shoc 2的分布 细胞中的信号复合物,并将大大加强这些研究,以检查Shoc 2的作用, 胚胎发育中的信号。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Emilia Galperin其他文献

Emilia Galperin的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Emilia Galperin', 18)}}的其他基金

Mechanisms and Functions of Shoc2-Transduced Cellular Signals
Shoc2 转导的细胞信号的机制和功能
  • 批准号:
    10387097
  • 财政年份:
    2020
  • 资助金额:
    $ 23.45万
  • 项目类别:
Mechanisms and functions of Shoc2-transduced cellular signals
Shoc2 转导的细胞信号的机制和功能
  • 批准号:
    10395494
  • 财政年份:
    2020
  • 资助金额:
    $ 23.45万
  • 项目类别:
Mechanisms and functions of Shoc2-transduced cellular signals
Shoc2 转导的细胞信号的机制和功能
  • 批准号:
    10613880
  • 财政年份:
    2020
  • 资助金额:
    $ 23.45万
  • 项目类别:
Diversity Supplement - Deciphering the Molecular Mechanisms Underlying Active Scaffolding
多样性补充 - 破译活性支架背后的分子机制
  • 批准号:
    9249209
  • 财政年份:
    2015
  • 资助金额:
    $ 23.45万
  • 项目类别:
Deciphering the molecular mechanisms underlying active scaffolding
破译活性支架背后的分子机制
  • 批准号:
    9279178
  • 财政年份:
    2015
  • 资助金额:
    $ 23.45万
  • 项目类别:
ROLE OF SHOC2 PROTEIN IN SPATIO-TEMPORAL REGULATION OF ERK KINASE CASCADE
SHOC2 蛋白在 ERK 激酶级联时空调控中的作用
  • 批准号:
    8360579
  • 财政年份:
    2011
  • 资助金额:
    $ 23.45万
  • 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
  • 批准号:
    8137875
  • 财政年份:
    2010
  • 资助金额:
    $ 23.45万
  • 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
  • 批准号:
    8114450
  • 财政年份:
    2010
  • 资助金额:
    $ 23.45万
  • 项目类别:
Regulation of MAPK Activity by EGFR Endocytosis
EGFR 内吞作用对 MAPK 活性的调节
  • 批准号:
    8322198
  • 财政年份:
    2010
  • 资助金额:
    $ 23.45万
  • 项目类别:
Regulation of MAP Activity by EGFR Endocytosis
EGFR 内吞作用对 MAP 活性的调节
  • 批准号:
    7683858
  • 财政年份:
    2008
  • 资助金额:
    $ 23.45万
  • 项目类别:

相似海外基金

Proton-secreting epithelial cells as key modulators of epididymal mucosal immunity - Administrative Supplement
质子分泌上皮细胞作为附睾粘膜免疫的关键调节剂 - 行政补充
  • 批准号:
    10833895
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
A Longitudinal Qualitative Study of Fentanyl-Stimulant Polysubstance Use Among People Experiencing Homelessness (Administrative supplement)
无家可归者使用芬太尼兴奋剂多物质的纵向定性研究(行政补充)
  • 批准号:
    10841820
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
StrokeNet Administrative Supplement for the Funding Extension
StrokeNet 资助延期行政补充文件
  • 批准号:
    10850135
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
2023 NINDS Landis Mentorship Award - Administrative Supplement to NS121106 Control of Axon Initial Segment in Epilepsy
2023 年 NINDS 兰迪斯指导奖 - NS121106 癫痫轴突初始段控制的行政补充
  • 批准号:
    10896844
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Biomarkers of Disease in Alcoholic Hepatitis Administrative Supplement
酒精性肝炎行政补充剂中疾病的生物标志物
  • 批准号:
    10840220
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Administrative Supplement: Life-Space and Activity Digital Markers for Detection of Cognitive Decline in Community-Dwelling Older Adults: The RAMS Study
行政补充:用于检测社区老年人认知衰退的生活空间和活动数字标记:RAMS 研究
  • 批准号:
    10844667
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Administrative Supplement: Improving Inference of Genetic Architecture and Selection with African Genomes
行政补充:利用非洲基因组改进遗传结构的推断和选择
  • 批准号:
    10891050
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Power-Up Study Administrative Supplement to Promote Diversity
促进多元化的 Power-Up 研究行政补充
  • 批准号:
    10711717
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Administrative Supplement for Peer-Delivered and Technology-Assisted Integrated Illness Management and Recovery
同行交付和技术辅助的综合疾病管理和康复的行政补充
  • 批准号:
    10811292
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
Administrative Supplement: Genome Resources for Model Amphibians
行政补充:模型两栖动物基因组资源
  • 批准号:
    10806365
  • 财政年份:
    2023
  • 资助金额:
    $ 23.45万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了