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Studies of Tissue Culture for Mangrove Plants

红树林植物组织培养的研究

基本信息

批准号：
03806011
负责人：
NISHIMURA Kazuo
金额：
$ 1.02万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Studies of callus induction for some mangrove species(Rhizophoraceae) were done, that had not been reported to succeed from it. Several experiments of redifferentiation were also done. Propagules of three mangrove species(Kandelia candel, Rhizophora stylosa and Bruguiera gymnorrhiza) used for experiments were collected from lriomote and Okinawa island, Okinawa prefecture, during 1991 and 92. Terminal and root merystem were also used for callus induction. Terminal and root merystem were also used for callus induction. Plant hormone and its concentration were 2,4-D and Kinetin, and these combination of 5^*10^<-5>, ^*10^<-6> and ^*10^<-7>M respectively. Culture media used was Murasige and Skoog and 0.8% agar. Both terminal and root merystem of K.candel were not recognized to induce callus tissue. Because these tissues were so labile that affected by the sterilization and died with in two weeks after transplanting. Only explant of propagule were able to recognize callus formation, especially from immature ones. Several different developing stage of propagule of R.stylosa and B.gymnorrhiza studied, were recognized to produce callus from the early or young ones. Explant from mature propagules of two species were not successful by the contamination of fungi or bacteria even under heavy sterilization. All three mangrove species were not able to form callus from terminal and root merystem, but from the propagules in early or young stage of development. These facts were confirmed by following several experiments. Concentration of hormonal treatments are also determined by the same experiments. Some experiments for redifferentiation from R.stylosa callus were done under combinations of 2.4-D and IAA, and Kinetine, and each three concentrations described above. Few adventituous bud were able to differentiate from callus tissues. This condition are now under examination.

对一些未见成功的红树植物进行了愈伤组织诱导研究。并进行了再分化实验。研究了1991年和1992年在冲绳县利里奥特和冲绳岛采集的3种红树（candelia canddel、Rhizophora stylosa和Bruguiera gymnorrhiza）的繁殖体。愈伤组织的诱导也采用了顶根分生系统。愈伤组织的诱导也采用了顶根分生系统。植物激素及其浓度为2,4- d和Kinetin，分别为5 *10^<-5>、^*10^<-6>和^*10^<-7>M的组合。培养基为Murasige、Skoog和0.8%琼脂。在愈伤组织诱导方面，芥花的顶根和根均未被发现。因为这些组织非常不稳定，受灭菌影响，在移植后两周内死亡。只有繁殖体外植体能够识别愈伤组织的形成，尤其是未成熟愈伤组织的形成。茎柱草和裸子根繁殖体的几个不同发育阶段都可以产生早幼愈伤组织。两种植物成熟繁殖体的外植体即使经过强力灭菌，也因真菌或细菌的污染而无法成功外植体。3种红树都不能从顶生和根生中形成愈伤组织，而是在发育的早期或幼期形成愈伤组织。这些事实在随后的几次实验中得到证实。激素治疗的浓度也由同样的实验决定。用2.4-D与IAA和Kinetine分别在上述3种浓度下进行了柱花草愈伤组织再分化的实验。很少有不定芽能从愈伤组织中分化出来。目前正在检查这种情况。